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last modified:
12-Nov-2008

 

ImageJ PlugIn collection for PerkinElmer UltraviewRS and ERS RAW-files

This is a collection of Plugins to import PerkinElmer UltraView(E)RS data into ImageJ.
 

Author:

A.Seitz ALMF @ EMBL Heidelberg

   
Source:

Perkin_ElmerPan.jar compatible with Image5D 1.1.5

Perkin_ElmerPan.jar campatible with Image5D 1.2.0
 

Installation: Make sure that your ImageJ is version 1.37g or later and that you use Java 1.5.0_06 or later.

Download the Perkin_ElmerPan.jar  files into your plugin folder of ImageJ (e.g. on my computer c:\ImageJ\plugins).  

In addition you need also to download
Image5D.jar to your plugin folder.
This is really important; otherwise the plugin
is not working properly.

If you want to browse/edit the source code you will have to unzip the jar file.

 

 

Description: PE Pannel

either   Plugins-->PerkinElmer
or          File -->Import

This opens a pannel with four buttons. The functionality of each is described below.

Open...

This opens the raw files acquired with a PerkinElmer Ultraview (E)RS.
The parameters (e.g. number of channels, number of slices, number of time points) of the image series are read from the cfg
/tim file. The raw-files are imported into a stack and afterwards automatically converted to an Image5D stack.

ATTENTION: If you created a time series of a large volume ImageJ might run out of memory!

How to increase your memory

Z-project...

This opens the raw files acquired with a PerkinElmer Ultraview (E)RS.
The parameters (e.g. number of channels, number of slices, number of time points) of the image series are read from the cfg
/tim file. The raw-files are imported into a stack and a Maximum Projection along the z-axis is performed for every time point and every channel. The resulting image-stack is converted to an Image5D stack.

Batch Z-projection...

You have to select a folder. The plugins works with all subfolders of this folder if they contain raw data from an PerkinElmer Ultraview (E)RS. The images of every folder are opened, a maximum intensity projection along the z-axis is performed and the resulting stack (one image per channel and time point) is saved as TIFF-stack (if you checked the tick box for saving in addition you also have the option to convert the resulting stack to 8-Bit).

File Info

Opens a window where you can find the meta information to the loaded image. 

 

 

Futher information

Just let me know if you have any ideas/needs for further plugins dealing with data import/handling of PerkinElmer Ultraview (E)RS files.

If you have problems in installing/using the plugins just let me know.
Please also don't hesitate to report bugs.

bugreport/suggestions