Emicobg
This program combines the sampled data to generate the
Gn 's for a selected subset of the particles. The output is
a file EMICOBG.DAT which contains the combined data
for the set.
Program prints the mean residual for each Z level and flags
components which have no values.
See the warnings about recycling below.
For combining images with different regions of resolution (for
example when combining images of different defocus) use ranges
of annuli for the different regions.
- Inputs are indicated with >'s
- >IBMAX, NBAND, ILIST, ISPREAD (3i,f)
- IBMAX - Number of annulae which are to be included in
calculation of the Gn's.
- NBAND - Number of bands for amplitude scaling.
- ILIST - output parameter >0 for detailed listing
- SPREAD -
the maximum inverse value of eigenvalue
allowed in calculation. The resolution is decreased
until this criterion is met. A summary of the
eigenvalue spectrum is printed at the end
of the run.
- > Number of particles (i)
- ------ for each particle
- > Particle number
- > min and max value of annulae used to calculate Gn (2i)
for this particle
com file for complete reconstruction
annotated com file for complete reconstruction
corresponding log file
- Current version - 26jul95
-
-
-
eigenvalue limit, the program
recycles to a lower number of parameters. In practice this means that some
of the coefficients of the BG expansion will not be calculated. Since the
BG expansion is over cylindrical rather than icosahedral coordinates, the
resulting map will NOT have exact icosahedral symmetry and, in extreme
cases, will resemble a badly distorted bowler hat. This can be corrected by
symmetrizing the result so that starting bowler hat density now looks like
an icosahedrally arranged bowler hat chimera. This does not increase the
biological significance of the result. Recycling is not a bug but a warning
that you should be less optimistic about your ability to extract silk purses from
sow's ears or 2A structures from 50A data.
Naturally when you are attempting a
single particle reconstruction you will always get this error and you will always
ignore it and you will NEVER completely believe the result and are honor
bound not to try to publish it!!!!
character overflows output format 26jul95 sdf
For very large data sets the number of parameters solved exceeds
1000 and so the number can no longer be written in the nice compact form
usually used for the output (i3 per entry). This gives an error but
execution continues and there is NO effect on the reconstructed density.
The proper response is not to worry but rather give thanks that you
have so much data...
Author: Stephen Fuller
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Last Updated: 30 July, 1995