Transgenic C. elegans strains generated in the Mattaj lab

Genotype:	unc-119(ed3) ruIs32[unc-119(+) pie-1::GFP::H2B] III; ojIs1[unc-119(+) pie-1::GFP::tbb-2]?.
Description:	Stable expression of GFP-histone H2B and GFP-beta-tubulin when grown at 16-25C.
		ruIs32 contains plasmid pAZ132. 
		Generated by crossing strains AZ212 (Praitis et al. 2001. Genetics 157:1217-1226) and 
		WH204 (Strome et al. 2001. Mol. Biol. Cell 12:1751-1764).
Reference:	Mol. Biol. Cell 13, 4355-4370.

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XA3502

Genotype:	unc-119(ed3) qaIs3502[unc-119(+) pie-1::YFP::lmn-1 pie-1::CFP::H2B] ?.
Description:	Stable expression of YFP-LMN-1 (CeLamin) when grown at 24-25C. Expression of
		CFP-histone H2B has been silenced. 
		Generated by microparticle co-bombardment of strain DP38 with plasmids pPAG6 and pUP9.
Reference:	Mol. Biol. Cell in press.

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Genotype:	ima-2(ok256)/+ I; ojIs1[unc-119(+) pie-1::GFP::tbb-2]?.
Description:	Strain carrying deletion allele of one of the three C. elegans importin alpha genes.
		Embryos from homozygous ima-2(ok256) worms show severe defects in mitotic spindle
		formation and nuclear envelope assembly. Maintain heterozygous population by propagating
		worms that give 25% sterile progeny or by single worm PCR.
		Homozygous for pie-1::GFP-beta-tubulin.  
		Generated by outcrossing ima-2(ok256) worms from The C. elegans Gene Knockout
		Consortium with N2 wild type males seven times followed by crossing with strain WH204
		(Strome et al. 2001. Mol. Biol. Cell 12:1751-1764).
Reference:	Mol. Biol. Cell 13, 4355-4370.

Embryo from homozygous ima-2(ok256) hermaphrodite - note the absence of a mitotic spindle.
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Genotype:	unc-119(ed3) qaEx3504[unc-119(+) pie-1::GFP::emr-1].
Description:	Expression of GFP-emr-1 (CeEmerin) when grown at 20C.
		Generated by microparticle co-bombardment of strain DP38 with plasmids pDP#MM051 (Maduro &
		Pilgrim 1995. Genetics 141:977-988) and pPAG7.
Reference:	Mol. Biol. Cell 13, 4355-4370.

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XA3507

Genotype:	unc-119(ed3) qaIs3507[unc-119(+) pie-1::GFP::lem-2] ?.
Description:	Stable expression of GFP-LEM-2 (CeMAN1) when grown at 20C.
		Generated by microparticle co-bombardment of strain DP38 with plasmids pDP#MM051 (Maduro &
		Pilgrim 1995. Genetics 141:977-988) and pPAG10.
Reference:	Mol. Biol. Cell in press.

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