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Plasmid pBS1479


This plamid is used for tagging proteins with the TAP tag but can also be used for tagging with two IgG-binding units of Staphylococcus aureus protein A associated or not with a TEV protease cleavage site. Among others, it contains the following features:
- the TAP tag;
- a TRP1 marker from Kluyveromyces lactis.
This plasmid does not replicate in yeast.


Details about the TAP method can be found on our web site. Seraphin Lab TAP method


Details about the tagging strategy can also be found in oligonucleotide design and in one of our publications (Puig et al., (1998) Yeast, 14, 1139-1146.

Reference: A generic protein purification method for protein complex characterization and proteome exploration
Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M. and Séraphin, B.
Nature Biotech., 17, 1030 - 1032 (1999).
                                         [Full text from publisher]
                                         [Abstract from PubMed]
                                         [Comment published in Science (and its Pubmed link)]

Map of plasmid pBS1479
Map of plasmid pBS1479


Deduced sequence of plasmid pBS1479 
     1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg  tttatttttc taaatacatt caaatatgta tccgctcatg 80    
    81 agacaataac cctgataaat gcttcaataa tattgaaaaa  ggaagagtat gagtattcaa catttccgtg tcgcccttat 160   
   161 tccctttttt gcggcatttt gccttcctgt ttttgctcac  ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240   
   241 tgggtgcacg agtgggttac atcgaactgg atctcaacag  cggtaagatc cttgagagtt ttcgccccga agaacgtttt 320   
   321 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg  tattatcccg tattgacgcc gggcaagagc aactcggtcg 400   
   401 ccgcatacac tattctcaga atgacttggt tgagtactca  ccagtcacag aaaagcatct tacggatggc atgacagtaa 480   
   481 gagaattatg cagtgctgcc ataaccatga gtgataacac  tgcggccaac ttacttctga caacgatcgg aggaccgaag 560   
   561 gagctaaccg cttttttgca caacatgggg gatcatgtaa  ctcgccttga tcgttgggaa ccggagctga atgaagccat 640   
   641 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg  gcaacaacgt tgcgcaaact attaactggc gaactactta 720   
   721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc  ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 800   
   801 gctggctggt ttattgctga taaatctgga gccggtgagc  gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 880   
   881 taagccctcc cgtatcgtag ttatctacac gacggggagt  caggcaacta tggatgaacg aaatagacag atcgctgaga 960   
   961 taggtgcctc actgattaag cattggtaac tgtcagacca  agtttactca tatatacttt agattgattt aaaacttcat 1040  
  1041 ttttaattta aaaggatcta ggtgaagatc ctttttgata  atctcatgac caaaatccct taacgtgagt tttcgttcca 1120  
  1121 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct  tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200  
  1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga  tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1280  
  1281 agcagagcgc agataccaaa tactgtcctt ctagtgtagc  cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1360  
  1361 tacatacctc gctctgctaa tcctgttacc agtggctgct  gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440  
  1441 gacgatagtt accggataag gcgcagcggt cgggctgaac  ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1520  
  1521 tacaccgaac tgagatacct acagcgtgag ctatgagaaa  gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1600  
  1601 ggtaagcggc agggtcggaa caggagagcg cacgagggag  cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680  
  1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg  ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1760  
  1761 gcggcctttt tacggttcct ggccttttgc tggccttttg  ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1840  
  1841 taaccgtatt accgcctttg agtgagctga taccgctcgc  cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920  
  1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc  gcgttggccg attcattaat gcagctggca cgacaggttt 2000  
  2001 cccgactgga aagcgggcag tgagcgcaac gcaattaatg  tgagttagct cactcattag gcaccccagg ctttacactt 2080  
  2081 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga  taacaatttc acacaggaaa cagctatgac catgattacg 2160  
  2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct  gGAGGATCCA tggaaaagag aagatggaaa aagaatttca 2240  
  2241 tagccgtctc agcagccaac cgctttaaga aaatctcatc  ctccggggca cttgattatg atattccaac tactgctagc 2320  
  2321 gagaatttgt attttcaggg tGAGctcAAA ACCGCGGCTC  TTGCGCAACA CGATGAAGCC GTGGACAACA AATTCAACAA 2400  
  2401 AGAACAACAA AACGCGTTCT ATGAGATCTT ACATTTACCT  AACTTAAACG AAGAACAACG AAACGCCTTC ATCCAAAGTT 2480  
  2481 TAAAAGATGA CCCAAGCCAA AGCGCTAACC TTTTAGCAGA  AGCTAAAAAG CTAAATGATG CTCAGGCGCC GAAAGTAGAC 2560  
  2561 AACAAATTCA ACAAAGAACA ACAAAACGCG TTCTATGAGA  TCTTACATTT ACCTAACTTA AACGAAGAAC AACGAAACGC 2640  
  2641 CTTCATCCAA AGTTTAAAAG ATGACCCAAG CCAAAGCGCT  AACCTTTTAG CAGAAGCTAA AAAGCTAAAT GGTGCTCAGG 2720  
  2721 CGCCGAAAGT AGACGCGAAT TCCGCGGGGA AGTCAACCTG  AAGCTTGATA TCGAATTCCT GCAGCCCGGG GGATCCACTA 2800  
  2801 GTTCTAGAGC GGCCGCGGAA CGATCATTCA CTATATATAT  ATCAATTTAT ATATACGTAT GTGTAATTGA AGAAAGATAC 2880  
  2881 GTTTTTTCCT CTATTGAGAG GCCTGCTGGA TGAATAGCTT  TACCTTTTCT AAATCCTTGA TACCATCAGT CTCTACTCCT 2960  
  2961 CCACTTACAT CGACACCGAT CGCATTTGGT AACATATTAA  TGGCAACAGA AACGTTATCA GGATTCAATC CACCAGCGAT 3040  
  3041 AATGAATTTT ATCTCGGGAT GACTTGCAGA CCAACTGGAA  ATTGCACTCC AATTCAATTT CTCACCAGTG CCACCTTCAC 3120  
  3121 CAGAATCGAA CAACGTCAGC ACATTGTCTA CGTGTTCATA  CAGGTCCAGT AGTAATTCAC AATCCTGTGG GAACTGGAAC 3200  
  3201 CTCTTAATGA TTGGAATTGA AGATGGGATC AAAGATCTGT  ATTCTTTAAT ATCTTCATCT CCATGTAATT GTATCACATC 3280  
  3281 TAGATTATAT TCGTGGTACA GTTGAAGGAC ATCATCAACG  GACTGATTTC TAAACACCCC GACTAGTTTA GTACCTTTCA 3360  
  3361 CGTTCTCTTG TTGGTGAACT GCAGTTGAAA TACCTTTCGC  AACAGATGAG TCAATGGTTC TTTTCCTACC GGGAACACAA 3440  
  3441 ATGATACCTA AGTAATCAGC ACCATCATCC ACAGCAGTCT  TTGCAGCTTC AACGGTTTGC AAACCACACA CTTTAACGAG 3520  
  3521 CATCACAGTA GATTTCGCAA GAGGGTAGCT CGCTCAGAGT  ACCCAAGTAA ATGATTAGTA AACTGATGTT TGATAGTTCA 3600  
  3601 ATTTTTCAAT GAAATAACCT TATATTAAAA TTGATATTAC  TATTATACAA AAATAAAGAA TAAAGGATTT GAGTTTATAC 3680  
  3681 ATAAAATACC ATTATTATTT GTTCAGTGAG AGATACCGGG  GTATATGGGA TGTGTGTAGT GATACCATGC AATCATGTAT 3760  
  3761 CAAACATggg cccggtaccc aattcgccct atagtgagtc  gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 3840  
  3841 gactgggaaa accctggcgt tacccaactt aatcgccttg  cagcacatcc ccctttcgcc agctggcgta atagcgaaga 3920  
  3921 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg  aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 4000  
  4001 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact  tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 4080  
  4081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc  taaatcgggg gctcccttta gggttccgat ttagtgcttt 4160  
  4161 acggcacctc gaccccaaaa aacttgatta gggtgatggt  tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 4240  
  4241 ctttgacgtt ggagtccacg ttctttaata gtggactctt  gttccaaact ggaacaacac tcaaccctat ctcggtctat 4320  
  4321 tcttttgatt tataagggat tttgccgatt tcggcctatt  ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4400  
  4401 ttttaacaaa atattaacgc ttacaattta g                                                       4431


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Author: Séraphin@embl-heidelberg.de Last Modified, November 9, 1999