Advanced Light Microscopy FacilityHCScreening Microscopy

53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress. Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Sølvhøj Pedersen R, Grøfte M, Chan KL, Hickson ID, Bartek J & Lukas J. Nat Cell Biol. 2011;13(3), 243-53.

Micropilot: automation of fluorescence microscopy–based imaging for systems biology. Conrad, C., Wünsche, A., Tan, T.H., Bulkescher, J., Sieckmann, F., Verissimo, F., Edelstein, A., Walter, T., Liebel, U., Pepperkok, R.,  & Ellenberg, J. Nat Methods. 2011; 8, 246–249.

CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held, M., Schmitz, M.H., Fischer, B., Walter, T., Neumann, B., Olma, M.H., Peter, M., Ellenberg, J. & Gerlich, D.W. Nat Methods. 2010; 7:747–754.

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J. Nature. 2010;464(7289):721-7.

Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging. Walter, T., Held, M., Neumann, B., Heriche, J.K., Conrad, C., Pepperkok, R. & Ellenberg, J. J Struct Biol. 2010; (170):1–9.

Identification of cholesterol-regulating genes by targeted RNAi screening. Bartz F, Kern L, Erz D, Zhu M, Gilbert D, Meinhof T, Wirkner U, Erfle H, Muckenthaler M, Pepperkok R, Runz H. Cell Metab. 2009;10(1):63-75.

RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Doil C, Mailand N, Bekker-Jensen S, Menard P, Larsen DH, Pepperkok R, Ellenberg J, Panier S, Durocher D, Bartek J, Lukas J, Lukas C. Cell 2009;136(3):435-46.

Work Flow for Multiplexing siRNA Assays by Solid-Phase Reverse Transfection in Multiwell Plates. Erfle, H., Neumann, B., Rogers, P., Bulkescher, J., Ellenberg, J. & Pepperkok, R. J Biomol Screen. 2008; (7):575-80.

EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. Tegha-Dunghu, J., Neumann, B., Reber, S., Krause, R., Erfle, H., Walter, T., Held, M., Rogers, P., Hupfeld, K., Ruppert, T., Ellenberg, J. & Gruss, O.J. J Cell Sci. 2008;121(Pt 10):1718-26.

Reverse transfection on cell arrays for high content screening microscopy. Erfle, H., Neumann, B., Liebel, U., Rogers, P., Held, M., Walter, T., Ellenberg, J. & Pepperkok, R. Nature Protocols 2007; (2): 392-399

High-throughput RNAi screening by time-lapse imaging of live human cells. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J. Nat Methods. 2006; (5):385-90.