Advanced Light Microscopy FacilityNews

10 April 2013 The Olympus TIRF system has been upgraded with the new Olympus IX-83 microscope stand and the new Olympus/Pecon incubator.
26 March 2013

Zeiss and ALMF offer a workshop on the Zeiss Lightsheet Z.1 (commercial SPIM system) on 18 April:


10:00 room 13-518: Presentation of the Zeiss Lightsheet Z.1
Dr. Sven Poppelreuther (Zeiss)
11:00 room 13-516A: General introduction of the Lightsheet Z.1,
13:00 room 13-516A: Hands-on with your own samples


The system will stay at EMBL for tests until May 1st. Please read the sample preparation guideline, if you want to try it with your own samples.

Please contact almf, if you would like to participate in the workshop and test the system or have any other questions.

25 February 2013 The Huygens Remote Manager (HRM) has been updated to version 3.0 (using Huygens core 4.3). It now offers an optimzed workflow and more features.
List of changes, user manual (to follow soon)
20 December 2012 ALMF offers basics in microscopy courses (EMBL internal) beginning of 2013.


The second basics course will be followed by an Imaris workshop (22.02.2013) and a Volocity workshop (25.2.2013). You can register for these workshops independently of the basics courses.

10 December 2012 New  Imaris version 7.6.1 available.
12 October 2012 New  Imaris version 7.6.0 available.
10 September 2012 On 25 + 26 September the ALMF hosts a workshop on Raman spectroscopy for living cells: BioRam - "Photonic fingerprinting".
27 August 2012 New  Imaris version 7.5.2 available.
24 August 2012 HRM server (deconvolution) upgraded, now running with 96 GB RAM and able to read Leica lif-files containing multiple image series.
10 July 2012 New  Imaris version 7.5.1 available.
30 June 2012 EMBL internal workshop on superresolution with Ground State Depletion Imaging Microscopy (GSDIM).

10 July 2012
9:30 -10:30 room 13-518, Wernher Fouquet (Leica Microsystems):
Theory and basics of Ground State Depletion Microscopy

10:30 – 13:00 Anna Szymborska (Ellenberg group), Hands-on:
Sample preparation for GSD microscopy (1 color, theory for 2 colors)

14:00 – 16:00 hands-on GSD microscopy group 1 / hands-on data analysis and drift correction group 2

16:00 – 18:00 hands-on GSD microscopy group 2 / hands-on data analysis and drift correction group 1

The lecture will be open for everybody interested.

Please register for the hands-on by email to almf@embl.de until Wednesday 4th July 18:00.

12 June 2012 EMBL internal Workshop on Second Harmonics Generation (SHG) Microscopy on 26 June. 10:00 Lecture "Introduction to Second Harmonics Generation Imaging", room 518, Yilmaz Niyaz, Carl Zeiss.

Please register by email to almf@embl.de, if you want to attend the hands-on.

28 April 2012 Volocity workshop on 21 - 22 of June 2012. Please contact almf(at)embl.de to register.
27  March 2012 Amira is now available on Workstation 1.
28 February 2012 New Imaris version 7.4.2 available.
20 February 2012 Workstation 1 has been replaced by a high-end computer.
1 February 2012 The PerkinElmer Ultraview RS will be discontinued by ALMF end of February 2012.
12 January 2012 The Leica SR GSD super resolution system (localization-based superresolution) is now available in the ALMF.
22 December 2011 The Zeiss LSM 510 FCS will be finally discontinued end of 2011.
22 December 2011 The Leica SR GSD super resolution system will return to ALMF after upgrade to commercial product beginning of January 2012.
21 December 2011 New  Imaris version 7.4.0 available.
9 December 2011 Huygens Remote Manager (HRM) updated to version 2.1
16 November 2011 Deadline extended: FREE access to advanced biological and biomedical imaging facilities across Europe during the Euro-BioImaging proof-of-concept studies.
20 October 2011 Volocity workshop on 2nd and 3rd of November. Please contact almf(at)embl.de to register.
1 October 2011 Leica SR GSD system available in the ALMF. This system uses ground state depletion for localization-based super-resolution.
1 October 2011 FREE access to advanced biological and biomedical imaging facilities across Europe during the Euro-BioImaging proof-of-concept studies.
29 September 2011 Yokogawa CV1000 spinning disk system (2 different pinhole sizes) will by available for testing from 17 - 28 October (in collaboration with Visitron).
21 September 2011 Olympus LV200 Bioluminescence microsope available in the ALMF.
15 September 2011 The Zeiss Spinning disk demo system will be disassembled on 26 September 2011.
11 September 2011 New  Imaris version 7.3.1 available.
5 September 2011 The software of the PerkinElmer Ultraview ERS system has been upgraded to a Volocity.
6 July 2011 New Imaris license server adress: 2700@10.11.4.35.
1 July 2011 Carl Zeiss LSM 780 NLO with GaAsP NDD detectors now available.
10 June 2011 Direct link to the Euro-BioImaging survey open until 15 July 2011.
25 May 2011 We are planning to discontinue the Zeiss LSM 510 FCS soon. Please inform us, if you need to finish experiments or an introduction for other systems.
3 May 2011 Imaris 7.2.3 available.
30 April 2011 Huygens remote manager (HRM) deconvolution available in version 2.0 now.
21 March 2011 Zeiss Spinning disk system available for test (~ next 3 months)
26 November 2010 New license path for Imaris due to license server relocation: 2700@10.11.113.100
15 November 2010 All ALMF systems have been succesfully relocated into building 13.
Overview of floor 5 in building 13
19 August 2010 We moved the image analysis workstations and the ALMF office to building 13 room 501 and 502.
26 July 2010 We start moving the ALMF microscopes one by one into our new rooms. We will inform users for each microscope separately in time before relocating the system.
1 August 2009 Olympus UV laser cutting widefield system for cell cutting and sample manipulation applications.
22 December 2008 Leica AF 7000 TIRF MC replaces the AF6000LX
17 November 2008 Zeiss LSM 710 available in the ALMF 
2 July 2008 FV1000 upgraded with motorized XY-stage and Z-drift compensation (ZDC) for multi-position time-lapse and autofocus
2 June 2008 Improvision PerkinElmer Ultraview VoX Spinning disk microscope available 
12 January 2008 The Olympus Cell^M has been upgraded to Cell^R and Scan^R