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If molecules with rapid kinetics are investigated, advanced features can be necessary for FRAP experiments :
- higher laser power to bleach faster (to minimize diffusion during bleaching)
- time optimized FRAP modules (switching delays between bleach and postbleach image aquisition should be minimized)
- small formats and fast acquisition speed
Long-term FRAP-experiments which explore molecules with very slow recovery characteristics also requires additional properties of the imaging system:
- To correct for laser-fluctuations the laser intensity can be quantified by ‘monitor diodes’ or transmission images.
- Tracking of the mobile photobleached cells using autofocus routines
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