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[Overview] [Experimental Setup] [Protocol] [Data Evaluation] [Examples] [Literature] |
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[Alignment] [Quantification] [Background] [Correction] [Normalization] [(Im-)Mobile Fractions] [Halftime] [Modelling] |
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Image Alignment During longer FRAP experiments, e.g. analyzing very slow molecules or molecules which bind to immobile structures, the bleached ROI can move over time. In order to obtain the right data in these cases it is essential to correct for this movement by alignment prior to the intensity quantification. Image Alignment can be done with ImageJ TurboReg (freeware) or the commercial tool Autoaligner (Bitplane AG, Switzerland).
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contact: Stefan Terjung last update: 02/06/04 |