Data Evaluation

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[Alignment] [Quantification] [Background] [Correction] [Normalization] [(Im-)Mobile Fractions] [Halftime] [Modelling]

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Depending on the experiment there are several data evaluation steps which have to be carried out before meaningful results can be achieved:

  1. Alignment of the images (only necessary if the regions of interest moved over time).
  2. Fluorescence intensity quantification (obtaining the raw data)
  3. Background subtraction
  4. Correct for laser fluctuations, photobleaching during acquisition (postbleach) and total fluorescence loss caused by the bleaching step
  5. Normalization
  6. Mobile/immobile fraction
  7. T halftime of the equilibration of bleached and unbleached molecules
  8. Theoretical models to additionally determine binding characteristics of the analysed molecule

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contact: Stefan Terjung           last update: 02/06/04