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[Overview] [Experimental Setup] [Protocol] [Data Evaluation] [Examples] [Literature] |
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[Preconditions] [Acquisition Bleaching] [Imaging Steps] [Prebleach] [Bleach] [Postbleach] |
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Preconditions Before meaningful FRAP data can be measured the following experiments should be carried out: First when using fluorescent protein constructs the cells should be checked for physiological and morphological alterations compared to control cells. Only cells which are ‘normal’ should be used for further analysis by FRAP. Bleaching characteristics have to be checked in fixed cells to determine bleach rates without movement of the probed molecule. |
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contact: Stefan Terjung last update: 02/06/04 |