[Overview] [Experimental Setup] [Protocol] [Data Evaluation] [Examples] [Literature]

[Alignment] [Quantification] [Background] [Correction] [Normalization] [(Im-)Mobile Fractions] [Halftime] [Modelling]

< back     next >

Obtaining the ‘raw data’

To determine the “raw FRAP data” the total or average pixel values in the bleached ROI has to be determined for each timepoint. This can be done with the most confocal operating software (e.g. Zeiss LSM, Leica LCS) or with other image processing software which can handle time series (e.g. the freeware ImageJ).

< back     next >

[EAMNET Home] [EAMNET News] [Introduction]

contact: Stefan Terjung           last update: 02/06/04