Molecular Dynamics 2004

Imaging Molecular Dynamics (EAMNET practical course)
EMBL Heidelberg, 19.-21.4.2004

This EAMNET teaching course focused on time-lapse microscopy of living cells and fluorescence recovery after photobleaching (FRAP) analysis of molecular mobility. The acquired data was analyzed by different movement and FRAP analysis tools. Have a look at the course program. There was another ‘Imaging Molecular Dynamics’ course scheduled 2.-4. March 2005.

Please select from the following resources (also see the online teaching module on FRAP):

Lectures

Live Specimen Microscopy (pdf 792 kb)
by J.Rietdorf (ALMF, EMBL Heidelberg)

LSM 510 in Live Cell Imaging
(8MB) by J. Lindenau (Carl Zeiss)

Introduction to FRAP (powerpoint) or pdf
by K. Miura (500kb, also see the FRAP teaching module)

Molecular Mechanisms of DNA Repair and Transcription studied by FRAP
by A. Houtsmuller (Josephine Nefkens Institute, Rotterdam; 1.5 MB)

please also take a look at the lectures of ‘Imaging Molecular Dynamics 2005’

Results

Time-lapse imaging and movement analysis (see example movie 1 + 2)

FRAP and FLIP-FRAP of cells stably transfected with androgen receptor
(see example movie 3)

FRAP of gpi-anchored GFP

Example movies

(1) VSVG time-lapse
(1 MB, this movie is rescaled, shortened and compressed for short download time)

(2) p24 time-lapse
(1 MB, this movie is rescaled, shortened and compressed for short download time))

(3) FLIP-FRAP example (300 KB)
In this experiment the lower part of the nucleus is bleached and the recovery of the bleach region (FRAP) and the fluorescence loss in the opposite region (FLIP) is monitored. For more details have a look in the results section

For further information please contact EAMNET

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