Overview on Techniques

Overview of microscope techniques and methods available at EAMNET laboratories:

Microscopy

  • Fast multi-channel time-lapse fluorescence microscopy
  • Multi-channel confocal microscopy
  • Multi-beam confocal microscopy
  • 2-Photon microscopy
  • Total internal reflection microscopy (TIRF)
  • Fluorescence correlation spectroscopy (FCS)

Methods

  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence loss in photbleaching (FLIP)
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence correlation spectroscopy (FCS)
  • Correlative Video-Light Electron Microscopy
  • Ion Imaging
  • Volume time-lapse and volume rendering
  • Deconvolution, video contrast enhancement
  • Particle tracking and colocalisation
  • Environmental control for live cell imaging
  • Microinjection
  • Fixation, Immunocytochemistry, sample preparation
  • Protein labeling and GFP fusions
  • Spectral Imaging / Emission Fingerprinting

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