|
This is a collection of macros to import and process image series in the generic (raw) file format of the Perkin Elmer Ultraview RS Software. The files are RAW files with a 6 byte offset and little-endian byte order.
The 3D stacks are loaded sequentially which enables the processing of large series even with small RAM.
Don´t use space characters in the path or filenames!
Functions:
Projections
Make Timelapse 1 channel:
This macro loads the raw data files of a 1 channel 2D time-lapse into one stack.
Make Timelapse 2 channel:
This macro loads the raw data files of a 2 channel 2D time-lapse into one stack for each channel
Make projections 1 channel:
This macro loads the raw data files of a 1 channel 3D time-lapse and z-projects it into one stack.
Make projections 2 channel:
This macro loads the raw data files of a 2 channel 3D time-lapse and z-projects it into one stack for each channel.
Make color coded projections 1 channel:
This macro loads the raw data files of a 1 channel 3D time-lapse and z-projects it depth color coded into one RGBstack.
Make color coded projections 2 channel:
This macro loads the raw data files of a 2 channel 3D time-lapse and z-projects it depth color coded into one RGBstack for each channel.
Export to Tiff-files
Batch Convert 3D 1 Channel (among 2 channels) to Tiff:
Batch Convert 3D 2 Channel to Tiff:
Converts a 3D 2 Channel data set (raw-files) to tiff-files. The user can choose a destination folder for each channel.
Batch Convert 3D 2 Channel to 8bit Tiff:
As above but with conversion to 8bit. The user supplies the upper and lower grayvalues of the original series which will correspond to 255 and 0 in the 8bit files, respectively.
Batch Convert 3D 2 Channel to half resized Tiff:
Also converts raw to tiff but the images are downscaled to 50% in x- and y-direction.
Batch Convert 3D 2 Channel to half resized 8bit Tiff:
Conversion from raw to tiff with downscaling to 50% and 8bit.
|
