Teaching Modules

 

 

 

Web-based  teaching modules developed by EAMNET members are available on this page. The teaching modules may be used without copyright restriction for academic purposes, PROVIDED YOU GIVE CREDIT TO THE AUTHOR AND REFERENCE THIS SITE. You may use teaching modules directly or modify them for your own purposes. Please remember to acknowledge the source of any slides used. However, they may not be reproduced for commercial purposes in any form without written permission of the author.

 

 

Module Title

Short Description

Author

Carl Zeiss - Microscopy from the beginning

Microscopy is not difficult – Using a microscope is a skill you can learn.
Sitting in front of a microscope might puzzling in the very beginning. The microscope with all of the screws, switches, mysterious numbers and color rings on the objectives may be confusing. And, to make it even worse, you might not even see a decent image when you look through it. Nevertheless, microscopy is easier than you may think. Everything is based on rules which never change. Once you have understood and used these rules in practice, success is bound to follow. Many years of practice and improvement and making your own creative changes to standard methods may also make you a master craftsman/woman in this field. This teaching module may help you to make a successful beginning.

Content of the teaching module

  • Fundamentals in microscopy (magnification, resolution, aperture)
  • The path of the light rays
  • “Köhler” illumination
  • Contrasting techniques in transmitted light (dark field, phase contrast, VAREL contrast, polarization contrast, differential interference contrast)
  • Fluorescence microscopy
  • Contrasting techniques in reflected light (darkfield, polarization contrast, differential interference contrast)
  • Optics for microscopes (condensers, objectives, eyepieces)
  • Photomicroscopy – documentation
  • Videomicroscopy

responsible:

R. Ankerhold

Calcium Imaging

Ions like Ca2+(Calcium), Cl- (Chloride) and Na+ (Sodium) play important roles in the cell, espacially in signalling events. This teaching module is the first on ion imaging in light microscopy. Also see info from last calcium imaging course.

M.Cruz,
S. Castel

Cell Motility

This presentation is an animated story of cell motility based on a collection of studies from the lab of Vic Small.

E. Vignal,
V. Small

Correlative Video-Light Electron Microscopy

This module describes an approach to combine the capability of in vivo fluorescence video microscopy with the resolution power of electron microscopy (EM). Based on the combination of these two techniques, by which an individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and then analyzed by EM and three-dimensional (3D) reconstruction methods.

A. Luini

Fluorescence Recovery After Photobleaching (FRAP)

This teaching module deals with the Fluorescence Recovery After Photobleaching (FRAP) technique. Also have a look at the talks given during the EAMNET course on ‘Imaging Molecular Dynamics’ and the FRAP Analysis macro as well as the documentation of this macro.

S. Terjung,
R. Pepperkok

Fluorescence Resonance Energy Transfer (FRET)

FRET techniques enable the detection of molecule-molecule interaction in the nanometer scale using light microscopes with dedicated features. This teaching module gives an introduction into FRET. Further Information can be found on the pages of the EAMNET course ‘Imaging Molecular Interactions and Reactions by FRET’. Use Acrobat 5.x.

S. Terjung,
T. Zimmermann

Quantitative Multidimensional Microscopy
(20 MB, also available as pdf (7 MB))

In this presentation we display representative examples of a series of novel applications of digital microscopy used for the study of cell-cell and cell matrix adhesions. These applications were used for the characterization of focal adhesions, their formation, forces applied to them, their composition and their signaling activity.

B. Geiger,
Z. Kam

 

 

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