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Module Title
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Short Description
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Author
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Carl
Zeiss - Microscopy from the
beginning
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Microscopy is not difficult – Using a
microscope is a skill you can learn.
Sitting in front of a microscope might puzzling
in the very beginning. The microscope with all of the screws, switches,
mysterious numbers and color rings on the objectives may be confusing. And,
to make it even worse, you might not even see a decent image when you look
through it. Nevertheless, microscopy is easier than you may think.
Everything is based on rules which never change. Once you have understood
and used these rules in practice, success is bound to follow. Many years of
practice and improvement and making your own creative changes to standard
methods may also make you a master craftsman/woman in this field. This
teaching module may help you to make a successful beginning.
Content of the teaching module
- Fundamentals in microscopy (magnification,
resolution, aperture)
- The path of the light rays
- “Köhler” illumination
- Contrasting techniques in transmitted light
(dark field, phase contrast, VAREL contrast, polarization contrast,
differential interference contrast)
- Fluorescence microscopy
- Contrasting techniques in reflected light
(darkfield, polarization contrast, differential interference contrast)
- Optics for microscopes (condensers,
objectives, eyepieces)
- Photomicroscopy – documentation
- Videomicroscopy
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responsible:
R.
Ankerhold
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Calcium Imaging
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Ions like Ca2+(Calcium),
Cl- (Chloride) and Na+ (Sodium) play important roles
in the cell, espacially in signalling events. This teaching module is the
first on ion imaging in light microscopy. Also see info from last calcium imaging course.
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M.Cruz,
S. Castel
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Cell Motility
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This presentation is
an animated story of cell motility based on a collection of studies from
the lab of Vic Small.
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E. Vignal,
V. Small
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Correlative Video-Light Electron Microscopy
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This module describes
an approach to combine the capability of in vivo fluorescence video
microscopy with the resolution power of electron microscopy (EM). Based on
the combination of these two techniques, by which an individual
intracellular structure can be monitored in vivo, typically through
the use of markers fused with green fluorescent protein (GFP), and then
analyzed by EM and three-dimensional (3D) reconstruction methods.
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A.
Luini
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Fluorescence Recovery After Photobleaching (FRAP)
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This teaching module
deals with the Fluorescence Recovery After Photobleaching (FRAP) technique.
Also have a look at the talks given during the EAMNET course
on ‘Imaging Molecular Dynamics’ and the FRAP Analysis macro as well as the documentation of this macro.
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S. Terjung,
R. Pepperkok
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Fluorescence Resonance
Energy Transfer (FRET)
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FRET techniques
enable the detection of molecule-molecule interaction in the nanometer
scale using light microscopes with dedicated features. This teaching module
gives an introduction into FRET. Further Information can be found on the
pages of the EAMNET course ‘Imaging Molecular Interactions and Reactions
by FRET’. Use Acrobat
5.x.
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S. Terjung,
T. Zimmermann
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Quantitative Multidimensional Microscopy
(20 MB, also
available as pdf (7 MB))
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In this presentation
we display representative examples of a series of novel applications of
digital microscopy used for the study of cell-cell and cell matrix adhesions.
These applications were used for the characterization of focal adhesions,
their formation, forces applied to them, their composition and their
signaling activity.
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B. Geiger,
Z. Kam
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