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Electron Tomography Toolbox for Amira

Watershed

Description:

The Watershed module is a segmentation tool for 3D data using a step function (Volkmann, N. (2002). A novel three-dimensional variant of the watershed transform for segmentation of electron density maps. J. Struct. Biol. 138: 123-129). It connects regions which have the similar values in one region and labels them with a unique number. From the threshold new labels will be generated and existing labels will be expanded. Pixels which have a higher value than Maximum_threshold_for_new_labels are labeled with 0. With the number of steps you can control the increasing value between threshold and Maximum_threshold_for_new_labels.
The segmentation process is started, when the Apply button is pressed.

Ports:

Threshold

From the number enterd here the labeling will be started. Regions below this threshold will have a unique number.

Steps

How many steps should be performed? The steps are used to overcome some noise and avoid creating a label for each noise spot.

Maximum threshold for new labels

Up to this number the image will be labeled. Every values above this number will be set to zero.

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