Protein Expression and Purification Core FacilityBiophysical Support

Dynamic Light Scattering (DLS) is based on measuring the time dependent fluctuation of scattered light intensity by macromolecular solution. This light intensity fluctuation can be transformed by non-linear fitting of autocorrelation function into the molecular size distribution. This method gives a possibility of calculating diffusion coefficient, hydrodynamic radius, molecular weight and polydispersity of investigated samples.