There are various factors that can affect the result of a protein purification experiment, e.g. lysis method and conditions, buffer composition, temperature, protein solubility.
Here you can find some information about the different steps in the course of a protein purification. If you have any further questions or comments, please contact the members of our group.
You can find more detailed information about protein purification techniques in the Handbook series published by GE Lifesciences (formerly known as Amersham Biosciences):
Please note that the principles and applications described in this series are not limited to the material that can be purchased from the publishing company.
An even more detailed collection of information on protein purification and more can be found on the website of the Protein Purification Facility of the Wolfson Centre for Applied Structural Biology (Hebrew University of Jerusalem).