The Proteomics Core Facility maintains and uses equipment for protein separation and imaging, as well as for sample preparation for mass spectrometry. We are equipped with state-of-the-art chromatography systems and mass spectrometers for LC, MS and LC-MS/MS, with a wide range of bioinformatic tools for data interpretation and evaluation.
LTQ Orbitrap Velos Pro (Thermo Fisher)
Hybrid linear ion trap-Orbitrap mass spectrometer. Used for protein identification and quantification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. Both CID (low resolution) and HCD (high resolution) options available for MS/MS fragmentation.
Q-Exactive (Thermo Fisher)
Hybrid quadrupole-Orbitrap mass spectrometer. Used for protein identification and quantification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. HCD (high resolution) fragmentation allows for use of MS2 quantification strategies (eg. TMT labelling).
Q-Tof 2 (Waters)
Quadrupole Time-of-Flight electrospray mass spectrometer. Used in combination with HPLC (C4 column) for mass determination of intact proteins
MALDI micro MX (Waters)
MALDI Time-of-Flight mass spectrometer. Used for protein identification by peptide mass fingerprinting (single proteins only)
NanoAcquity UPLC System (Waters)
Nano LC System - used for peptide separation interfaced directly to the Orbitraps and Triple Quad for LC-MS/MS
Ultimate 3000 RSLC System (Dionex)
Nano LC System - used for peptide separation interfaced directly to the ion traps and the Q-Exactive for LC-MS/MS
Offline Sample Fractionation
1200 Infinity HPLC (Agilent)
Offline analytical HPLC system with quarternary pumps, variable wavelength UV detector and micro fraction collector. For pre-fractionation of complex protein digests prior to LC-MS/MS. Operates in either High pH reverse phase or SCX mode.
Protein Separation and Imaging
2D Gel Equipment
Densitometer (GS-800, Bio-Rad)
Fluorescence imager (Molecular Imager FX, Bio-Rad)
UV Spectrometer (SmartSpec 3000, Bio-Rad)
Mascot (Matrix Science)
Proteome Discoverer (Thermo Fisher)
MaxQuant (Max Planck Institute of Biochemistry, Martinsried, Germany)
Peaks Studio (Bioinformatics Solutions Inc.)
Progenesis LC-MS (Nonlinear Dynamics Ltd)
Scaffold (Proteome Software)
Protein Lynx Global Server - PLGS (Waters)