Proteomics Core FacilityInstrumentation
The Proteomics Core Facility is equipped for protein separation and imaging, sample preparation for mass spectrometry, mass spectrometers for MS and LC-MS/MS, and bioinformatic tools for data interpretation and evaluation.
Liquid chromatography and Mass Spectrometry
NanoAcquity UPLC System (Waters)
Nano LC System - used for peptide separation interfaced directly to the Orbitrap for LC-MS/MS
Ultimate 3000 RSLC System (Dionex)
Nano LC System - used for peptide separation interfaced directly to the ion traps and the MaXis for LC-MS/MS
LTQ Orbitrap Velos (Thermo Fisher)
Used for protein identification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. Both CID (low resolution) and HCD (high resolution) options available for MS/MS fragmentation.
Ion trap mass spectrometer (HCT Ultra, Bruker)
Used for protein identification by LC-MS/MS
MaXis UHR-TOF system (Bruker)
Used for protein identification by LC-MS/MS with high mass accuracy
Quadrupole Time of Flight electrospray tandem mass spectrometer (Q-Tof 2, Waters)
Used for mass determination of intact proteins
MALDI Time of Flight mass spectrometer (MALDI micro MX, Waters)
Used for protein identification by peptide mass fingerprinting (single proteins only)
Protein Separation and Imaging
2D Gel Equipment
Densitometer (GS-800, Bio-Rad)
Fluorescence imager (Molecular Imager FX, Bio-Rad)
UV Spectrometer (SmartSpec 3000, Bio-Rad)
Robotic Sample Preparation
Spot cutting robot (EXQuest, BIO RAD)
Used to cut spots from 2D-gels in an automated fashion
Software
Protein Lynx Global Server - PLGS (Waters)
ProteinScape (Bruker)
Scaffold (Proteome Software)