The Proteomics Core Facility maintains and uses equipment for protein separation and imaging, as well as for sample preparation for mass spectrometry.  We are equipped with state-of-the-art chromatography systems and mass spectrometers for LC, MS and LC-MS/MS, with a wide range of bioinformatic tools for data interpretation and evaluation. 

Mass Spectrometry

Orbitrap Fusion ETD

Orbitrap Fusion ETD

Tribrid linear ion trap-quadrupole-Orbitrap mass spectrometer. The tribrid architecture, with an independent ion-routing multipole which enables parallelisation, significantly increases scan rates, and maximises versatility. Any of three dissociation techniques — CID, HCD, and ETD — can be performed at any fragmentation stage, followed by analysis in either the linear ion trap or Orbitrap mass analyzer.

LTQ Orbitrap Velos Pro (Thermo Fisher)

Hybrid linear ion trap-Orbitrap mass spectrometer.  Used for protein identification and quantification by LC-MS/MS.  Principally used for complex samples and identification of post-translational modifications.  Both CID (low resolution) and HCD (high resolution) options available for MS/MS fragmentation.   

QE_s

Q-Exactive Plus (Thermo Fisher)

Hybrid quadrupole-Orbitrap mass spectrometer.  Used for protein identification and quantification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. HCD (high resolution) fragmentation allows for use of MS2 quantification strategies (eg. TMT labelling).

Q-Tof Premier (Waters)

Quadrupole Time-of-Flight electrospray mass spectrometer. Used in combination with HPLC (C4 column) for mass determination of intact protein

QQQ_s

TSQ Vantage (Thermo Fisher)

Triple quadrupole mass spectrometer used for targeted quantification

Liquid Chromatography

TOP

NanoAcquity UPLC System (Waters)

Nano LC System — used for peptide separation interfaced directly to the Orbitraps and Triple Quad for LC-MS/MS

Ultimate 3000 RSLC System (Dionex)

Nano LC System — used for peptide separation interfaced directly to the ion traps and the Q-Exactive for LC-MS/MS

Offline Sample Fractionation

Agilent_s

1200 Infinity HPLC (Agilent)

Offline analytical HPLC system with quarternary pumps, variable wavelength UV detector and micro fraction collector. For pre-fractionation of complex protein digests prior to LC-MS/MS. Operates in High pH reverse phase mode.

Protein Separation and Imaging

SDS PAGE equipment

Calibrated Densitometer (GS-900, Bio-Rad)

Software

Mascot (Matrix Science)

Proteome Discoverer (Thermo Fisher)

MaxQuant (Max Planck Institute of Biochemistry, Martinsried, Germany)

Scaffold (Proteome Software)

ProteinScape (Bruker)

IsobarQuant (Franken H. et al. Nature Procotols, 2015)