Proteomics Core FacilitySample Preparation

How to send a cut gel band

If you are going to cut out the gel bands yourself from a gel, please follow carefully the following instructions:

  • ONLY cut gels on a clean glass plate (never use overhead projector foils)
  • Wear gloves at all times
  • Use a NEW clean scalpel blade for precise cutting

Gel_1_s

  • PRECISELY cut out ONLY the band - any excess gel will lead to background noise

Gel_2_s

  • Chop the gel band into approx 1 mm squares

Gel_3_s

  • Place the gel cubes in a clean eppendorf tube (500 µL) and add enough water just to cover
  • Check the lid is shut before placing in an envelope to send.
  • Please DO NOT use parafilm around the lids