Our work focuses on the development and application of mass spectrometric tools for the proteomic interrogation of biological processes. We combine analytical chemistry and biochemistry with nano-flow chromatography and mass spectrometry for the enrichment, characterization and quantitation of proteins in complex samples. This mainly serves to understand how cellular dynamics is regulated by proteins and their modifications.
Orthogonal separation techniques for the characterization of the yeast nuclear proteome. S. Gauci, L.M. Veenhoff, A.J.R. Heck, and J. Krijgsveld. J. Proteome Res. (2009) (in press)
Optimizing identification and quantitation of 15N-labeled proteins in comparative proteomics. J.W. Gouw, B.B.J. Tops, P. Mortensen, A.J.R. Heck and J. Krijgsveld. Analytical Chemistry (2009) 80:7796-7803
An experimental correction for arginine-to-proline conversion-artifacts in SILAC-based quantitative proteomics. D. Van Hoof, M.W.H. Pinkse, D. Ward-Van Oostwaard , C.L. Mummery, A.J.R. Heck, and J.Krijgsveld. Nature Methods (2007) 4(9):677-678.