Structural Light Microscopy - Single Molecule Spectroscopy
For the study of biological mechanisms, protein engineering has become an essential tool. With genetically encoding non natural amino acids as our central strategy, we recruit a large plethora of novel chemical biology techniques to micro-manipulate biological function. A particular focus is the introduction of small, minimally perturbing fluorescent probes site selectively into proteins to study molecular structure and dynamics.
General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer. Brustard EM, Lemke EA, Schultz PG, Deniz AA, A, J Am Chem Soc, 52,17664-5 (2008)
Control of protein phosphorylation with a genetically encoded photocaged amino acid. Lemke EA, Summerer D, Geierstanger B, Brittain S, Schultz PG, Nature Chem Biol 3, 769-772 (2007)
Single-molecule biophysics: at the interface of biology, physics and chemistry. Deniz AA, Mukhopadhyay S, Lemke EA, J R Soc Interface , 5(18), 15-45 (2008)