Microfluidic systems for the screening and synthesis of small molecule libraries
The Merten Group develops novel screening and synthesis approaches based on microfluidic systems. For example, we have established tailored systems for the screening of one-bead-one-compound (OBOC) libraries. In parallel, we are developing microfluidic modules for on-chip combinatorial synthesis of highly diverse compound libraries (e.g. making use of click chemistry reactions). Compared to conventional approaches, this should result in drastically increased throughput and minimized consumables costs. Hence large areas of chemical structure space can be explored in search of new bioactive molecules. For this purpose we also develop novel assays coupling a desired chemical or biological property with a positive fluorescence signal.
Miniaturization and Parallelization of Biological and Chemical Assays in Microfluidic Devices. Vyawahare, S., Griffiths, A.D. & Merten, C.A. Chem Biol. 2010 Oct 29;17(10):1052-65.
Nanoliter plates--versatile tools for the screening of split-and-mix libraries on-bead and off-bead. Upert, G., Merten, C.A. & Wennemers, H. Chem Commun (Camb). 2010 Apr 7;46(13):2209-11. Epub 2010 Feb 23.
(selected as a "Hot Paper")
Imaging of mRNA in live cells using nucleic acid-templated reduction of azidorhodamine probes. Pianowski, Z., Gorska, K., Oswald, L., Merten, C.A. & Winssinger, N. J Am Chem Soc. 2009 May 13;131(18):6492-7.
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