Chemical Cell Biology
The Schultz lab performs preparative scale organic synthesis with a focus on lipid and inositol chemistry. We are specialists in protecting group chemistry and in synthesizing membrane-permeant, bioactivatable compounds (prodrugs). In addition, we have an expertise in phosphorylation chemistry and fluorescently labeling small molecules. We are currently developing new devices for in vivo fluorescent labeling and for switching enzyme activities by light and chemicals. The resulting compounds are used to dissect and visualize intracellular signaling networks in living cells. We are particularly interested in G-protein coupled and growth factor receptor signaling networks. We generally focus on events within 30 min after receptor activation including secretion and receptor internalization. In addition, we prepare small molecule-based fluorescent probes for measuring protease and lipase activities, which requires peptide and lipid synthesis expertise.
Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation. Cobos-Correa, A., Trojanek, J.B., Diemer, S., Mall, M.A. & Schultz, C. Nat Chem Biol. 2009;5(9):628-630.
Selective fluorescent labeling of lipids in living cells. Neef, A. B., Schultz, C. Angew Chem Int Ed. 2009;48:1498-1500.
Imaging the enzyme-substrate intermediate reveals functional subpopulations of the tyrosine phosphatase PTP1B in living cells. Yudushkin, I. A., Schleifenbaum, A., Neel, B. J., Schultz, C., Bastiaens, P. I. H. (2007). /Science /*315*/, /115-119.