Physics and Engineering at EMBL
Structural Light Microscopy - Single Molecule Spectroscopy
Single molecule, advanced imaging and super resolution microscopy are continuously developing techniques. To accommodate with current and future developments in the field and constantly maintain state of the art technologies, we build most fluorescence microscope and spectroscopy equipment our self and continue to develop such technologies. Furthermore, we are active in building of microfluidic technologies to assist our fluorescence techniques.
Microfluidic device for single-molecule experiments with enhanced photostability. Lemke EA, Gambin Y, Vandelinder V, Brustad EM, Liu HW, Schultz PG, Groisman A, Deniz AA. J Am Chem Soc. 2009 Sep 30;131(38):13610-2.
Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence. Ferreon AC, Gambin Y, Lemke EA, Deniz AA. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5645-50. Epub 2009 Mar 17.
Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity. Lemke EA, Klingauf J. J Neurosci. 2005 Nov 23;25(47):11034-44.