Seminar Colour Guide:              
External Faculty Speaker
Tuesday, 9 February 2016, 09:00 Add to calendar
Biallelic genome editing of human pluriopotent stem cells at scale
Bill Skarnes, Wellcome Trust Sanger Institute, United Kingdom Host: Vladimir Benes
ATC - Flex Labs A+B , EMBL Heidelberg
External Faculty Speaker
Wednesday, 10 February 2016, 11:00 Add to calendar Using recombineering to extend the power of CRISPR/Cas9 A. Francis Stewart, Technische Universität, Dresden, Germany Host: Vladimir Benes
ATC - Flex Labs A+B , EMBL Heidelberg
Seminar given by an external postdoc
Thursday, 11 February 2016, 11:00 Add to calendar In vivo mapping of the Mediator complex and implications for its role in transcription regulation Sebastian Gruenberg, Fred Hutchinson Cancer Research Center, USA Host: Lars Velten and Lars Steinmetz
Small Operon, EMBL Heidelberg
Abstract: A key step in regulation of protein encoding genes is the recruitment of coactivator complexes by gene-specific activators. Once recruited, coactivators facilitate transcription initiation by RNA polymerase II (Pol II) through recruitment of general transcription factors and rearrangement of chromatin at gene promoters. The coactivator complex Mediator is required for all cellular Pol II transcription and interfaces with both transcription activators and the basal transcription machinery.
Despite its central role in transcriptional initiation, the in vivo function of Mediator has remained controversial, due in large part to conflicting data about its genome-wide binding generated by ChIP-based methods. In my talk, I will present the development and use of chromatin endogenous cleavage and high-throughput sequencing (ChEC-seq), a method completely orthogonal to ChIP, to probe the in vivo binding of Mediator to the genome.

For ChEC, Mediator subunits Med8, Med17, and Med20 were fused to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo, followed by high-throughput sequencing of natively cleaved DNA fragments. While Med20-MNase did not cleave DNA, Med8- and Med17-MNase showed appreciable levels of cleavage, allowing for high-resolution, background free, determination of Mediator binding sites genome wide

I will present yet unpublished data showing that Mediator preferentially associates with upstream activating sequences (UASs) rather than core promoters or, as previously proposed, in gene bodies. Our data show striking differences in transcription complex formation at promoters regulated by coactivators SAGA or TFIID. We also find evidence that Mediator binding might have broader implications for genome architecture and suggests a function for Mediator beyond its role as co-activator.
Seminar given by an external postdoc
Friday, 12 February 2016, 13:00 Add to calendar A Dream: Towards confining a 3D amyloid model from solid-state NMR with single fiber diffraction data from free electron lasers Carolin Seuring, Center for Free Electron Laser Science, Hamburg, Germany Host: Annabel Parret
Seminar Room 48e, EMBL Hamburg
EMBL Distinguished Visitor Lecture
Monday, 15 February 2016, 11:00 Add to calendar Nutritional Regulatory Networks Marian Walhout, University of Massachusetts Medical School, USA Host: Anne-Claude Gavin
Large Operon, EMBL Heidelberg
Abstract: Gene regulation and metabolism lie at the heart of most biological processes. Both are accomplished by complex networks harboring hundreds of nodes and thousands of edges. We study these networks and the interactions between them mainly in the nematode C. elegans, because it is amenable to high-throughput, large-scale genetics and genomics. In addition, we study interspecies network interactions between C. elegans and bacteria, that may help illuminate interactions between mammalian intestinal cells and the gut microbiota.
EMBL Distinguished Visitor Lecture
Tuesday, 16 February 2016, 11:00 Add to calendar Three short stories about sex chromosomes Job Dekker, University of Massachusetts Medical School, USA Host: Yad Ghavi Helm, Aleksandra Pekowska
Large Operon, EMBL Heidelberg
Abstract: The 3D organization of the genome is critical for gene regulation. I will present three examples where sex chromosomes can serve as powerful models to study the folding of chromosomes in general, to identify cis-elements and proteins involved and to determine how chromosome organization and gene regulation are mechanistically linked.
EMBL Distinguished Visitor Lecture
Tuesday, 16 February 2016, 11:00 Add to calendar Organizing bacterial chromosomes: Towards a mechanism of action for SMC condensin Stephan Gruber, Max Planck Institute of Biochemistry, Martinsried,, Germany Host: Kyle Muir
EMBL Seminar Room, EMBL Grenoble
Science and Society
Tuesday, 16 February 2016, 18:00 Add to calendar Meine Darmmikroben und ich: eine innige, aber verwundbare Beziehung Peer Bork, EMBL Heidelberg, Germany Host: Halldór Stefánsson
Print Media Academy
Abstract: Das menschliche Mikrobiom ist die Gesamtheit aller Mikroben, die in oder auf uns leben und somit den Menschen als Ökosystem fungieren lassen, in dem mehr bakterielle als menschliche Zellen zu finden sind. Die unsichtbare Vielfalt der Mikroben war der Forschung lange Zeit nicht zugänglich, kann aber seit kurzem durch massives Sequenzieren des Erbmaterials von Lebewesen (Metagenomik), z.B. in menschlichen Proben wie Hautabstrich oder Stuhl, abgeschätzt und analysiert werden.

Der prominenteste bakterielle Lebensraum im Menschen ist der Darm, mit durschschnittlich 1,5kg mikrobieller Biomasse, zu der über 1000 verschiedene bakterielle Spezies beitragen, welche wichtige Funkionen für uns übernehmen, aber in seltenen Fällen auch Krankheiten verursachen. Über Infektionen hinaus wurden Mikroben bislang mit mehr als 30 verschiedenen Krankheiten assoziiert und sind somit auch potentielle diagnostische Biomarker, wie ich am Beispiel von Darmkrebs illustrieren werde (Zeller et al. Mol. Sys. Biol., 2014). Während die Hoffnung besteht, dass Abweichungen von der Normalzusammensetzung der Bakterien im Krankheitsfall in aller Welt gleich sind, bleibt die Charakterisierung des Normalen schwierig. Wir fanden vor einigen Jahren heraus, dass in vielen Ländern drei bevorzugte Bakteriengemeinschaften vorherrschen (Enterotypen; Arumugam et al., Nature, 2011), unabhängig von Alter, Geschlecht, BMI oder Herkunft. Diese drei Enterotypen haben möglicherweise Einfluss auf Verdauungseigenschaften oder Medikamentenverträglichkeit. Obwohl die Grobzusammensetzung der Mikrobengemeinschaften in Enterotypen einteilbar ist, sind wir auf der Bakterienstammebene ganz individuell. Die meisten von uns besitzen z.B. das Darmbakterium E.coli, jeder hat jedoch ein paar individuelle Mutationen oder Bakteriengene (Schloissnig et al., Nature, 2013; Zhu et al., Genome Biol. 2015), die unsere Bakteriengemeinschaften individuell auf externe Einflüsse reagieren lassen. Einige Bakteriengene sind z. B. die Träger von Antibiotikaresistenzen und auch diese sind in ihrer Gesamtheit recht individuell und quantifizierbar, wobei es hier große regionale Unterschiede gibt.

Die Individualität unseres Darm-Mikrobioms kann man sich zunutze machen, um z.B. zu verfolgen, was bei erfolgreichen mikrobiellen Therapien wie der Stuhltransplantation eigentlich passiert und wie man dies für die Verbesserung der Behandlung einsetzen kann. Die Sensitivität der Metagenomik ist enorm man kann Bakterienstämme nachweisen, die einem die verspeiste Joghurtsorte verraten oder Pathogene, auch wenn keine Symptome vorhanden sind. Nahrungs- oder Medikamenteneinnahme verändern unsere Mikrobiota, die Forschung ist jedoch in vielen Bereichen erst am Anfang und oft weiß man noch nicht, welche Zusammensetzung gut oder schlecht für den Einzelnen ist und welche Konsequenzen Veränderungen nach sich ziehen. Trozdem ist bereits abzusehen, dass die Mikroben nicht nur für Diagnose und Therapie von Krankheiten nützlich sein werden, sondern dass unsere Symbiose mit den Bakterien durch zielgerichtete Veränderung mittels Diät oder Nahrungszusätze für Gesundheit und Wohlbefinden verbessert werden kann.
EMBL Distinguished Visitor Lecture
Wednesday, 17 February 2016, 16:00 Add to calendar Structural biology and Design of New Medicines: Fighting the Emergence of Drug Resistance in Cancer and Tuberculosis Prof. Sir. Tom Blundell, Department of Biochemistry , University of Cambridge,, United Kingdom Host: Taiana maia de Oliveira
EMBL Seminar Room, EMBL Grenoble
Hamburg Speaker
Friday, 19 February 2016, 13:00 Add to calendar The dimensions of disordered proteins: insights from experiments, theory and simulations Gustavo Fuertes Vives, EMBL Hamburg, Germany Host: Dmitri Svergun
Seminar Room 48e, EMBL Hamburg
EMBL Distinguished Visitor Lecture
Monday, 22 February 2016, 11:00 Add to calendar A fly story: piRNAs for transposon defense Chantal Vaury-Zwiller, Laboratoire GReD - Génétique Reproduction & Développement, Faculté de Médecine, CLERMONT-FERRAND, France Host: Ramesh Pillai
EMBL Seminar Room, EMBL Grenoble
External Faculty Speaker
Friday, 26 February 2016, 11:00 Add to calendar To be announced Dean Mobbs , Psychology Department, Columbia University, New York, NY, USA Host: Cornelius Gross
NEW CNR Seminar Room, EMBL Monterotondo
External Faculty Speaker
Friday, 26 February 2016, 13:00 Add to calendar To be announced Kay Gruenewald, CSSB, Hamburg / University of Oxford, UK, United Kingdom Host: Matthias Wilmanns
Seminar Room 48e, EMBL Hamburg
Seminar given by an external postdoc
Thursday, 3 March 2016, 14:15 Add to calendar To be announced Michaela Müller-McNicoll, Goethe University Frankfurt, Germany Host: Janosch Hennig
Small Operon, EMBL Heidelberg
Tags: Biocomputing, Structural Biology
Hamburg Speaker
Friday, 4 March 2016, 13:00 Add to calendar To be announced Michele Cianci, EMBL Hamburg, Germany Seminar Room 48e, EMBL Hamburg
EMBL Distinguished Visitor Lecture
Monday, 7 March 2016, 11:00 Add to calendar To be announced C. David Allis, Tri-Institutional Professor, Joy and Jack Fishman Professor, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, USA Host: Predocs: Niccolo Arecco and Kyung-Min Noh
Large Operon, EMBL Heidelberg
External Faculty Speaker
Thursday, 10 March 2016, 11:00 Add to calendar Effects of histone core mutations on nucleosomal dynamics - studies by molecular dynamics modeling and single molecule fluorescence. Joerg Langowski, German Cancer Research Center (DKFZ), Biophysics of Macromolecules, Germany Host: Karin Sasaki/Stephanie Alexander
Small Operon, EMBL Heidelberg
Tags: Biophysics
External Faculty Speaker
Friday, 11 March 2016, 10:00 Add to calendar To be announced Massimiliano Pagani, Professor, of Molecular Biology, Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano; Head of Integrative Biology, INGM, Istituto Nazionale Genetica Molecolare Romeo ed Enrica Invernizzi , Milano, Italy Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
EMBL Distinguished Visitor Lecture
Friday, 11 March 2016, 11:00 Add to calendar To be announced Kiyoshi Nagai, MRC LMB Cambridge, United Kingdom Host: Stephen Cusack
EMBL Seminar Room
Seminar given by an external postdoc
Friday, 18 March 2016, 13:00 Add to calendar Quantitative proteomics strategies to analyze molecular mechanisms of neurodegeneration Daniel Hornburg, Max Planck Institute of Biochemistry, Munich, Germany Host: Postdocs / Anne-Sophie Huart
Seminar Room 48e, EMBL Hamburg
Science and Society
Friday, 18 March 2016, 15:00 Add to calendar The infinite foetus: connecting person and placenta through time and space Julienne Rutherford, University of Illinois, Chicago, USA Host: Halldór Stefánsson
Large Operon, EMBL Heidelberg
Abstract: The foetus as a biological entity has labile boundaries. 1) It is an individual with its own genome, but that genome is the collaborative output of two other individuals, which in turn exponentialises into past generations. 2) The watery world in which it develops also has a temporal signature that reaches into the past and extends beyond gestation, through phenomena that conceptually coalesce within the Developmental Origins Paradigm. 3) The foetus as an entity does not exist without its placenta. While the placenta is an extrasomatic foetal organ it must be conceptually incorporated with the foetus as the biological bridge between generations. A biological view of the foetal experience that is restricted to the time and space of the foetus s body alone is inadequate to fully situate individuals, communities, and species within the intergenerational ecologies they create and inhabit. Framing the foetus as the fruit of previous generations and the seed from which future generations grow gives rise to a biology of life history that is Moëbian rather than linear. Data and impressions from Dr. Rutherford s work with marmoset monkeys, vervet monkeys, and humans will illuminate the themes discussed above.
EMBL Distinguished Visitor Lecture
Tuesday, 22 March 2016, 10:00 Add to calendar Disentangling neuronal circuits for motor control Silvia Arber, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
Abstract: Abstract:
Movement is the behavioral output of the nervous system. Animals carry out an enormous repertoire of distinct actions, spanning from seemingly simple repetitive tasks like walking to more complex movements such as forelimb manipulation tasks. This lecture will focus on recent work elucidating the organization and function of neuronal circuits at the core of choosing, maintaining, adjusting and terminating distinct motor behaviors. It will show that dedicated circuit modules within different brainstem nuclei and their interactions in the motor system play key roles in action diversification.

Short Bibliography:
Silvia Arber is a Professor of Neurobiology at the Biozentrum of the University of Basel and a Senior Investigator at the Friedrich Miescher Institute for Biomedical Research (FMI) in Basel. Her research is focused on neuronal circuits controlling motor behavior. Arber studied biology at the Biozentrum and graduated with a doctorate in the laboratory of Pico Caroni at the FMI in 1995. After a postdoctoral fellowship in the laboratory of Thomas Jessell at Columbia University in New York, she returned to Basel in 2000 to establish her independent research group at the Biozentrum and the FMI. She has been recognized for her research with numerous prizes, including the Pfizer Research Prize (1998), the National Latsis Prize (2003), the Schellenberg Prize and the Friedrich Miescher Award (2008), the Otto Naegeli Prize 2014), as well as the Premio Remedios Caro Almela (2015).
External Faculty Speaker
Tuesday, 22 March 2016, 11:00 Add to calendar Modeling biological signaling with spatial and stochastic detail. Steve Andrews, Fred Hutchinson Cancer Research Centre, USA Host: Karin Sasaki
Small Operon, EMBL Heidelberg
Abstract: All biological cells sense their environment using cell signaling, in which chemicals bind to receptors, the receptors initiate a chain of events, and these events initiate cellular responses. Experimental biologists have mapped out many of these signaling pathways by now. However, the reason for the pathway designs, and how well they actually function, are largely open questions. I am pursuing these topics using computational modeling. In particular, I developed software for simulating the reaction and diffusion dynamics of tens of thousands of individual biomolecules (the Smoldyn program, which is widely used, and which I will also present a course on). I also quantified information transfer in yeast cell signaling using experimental data. I found that yeast signaling, and likely others, is much more precise than previously believed. Tags: Cell Biology
EMBL Distinguished Visitor Lecture
Thursday, 24 March 2016, 11:00 Add to calendar To be announced Prof. David Rueda, Imperial College,London, United Kingdom Host: Stefan Reich
EMBL Seminar Room
Hamburg Speaker
Friday, 8 April 2016, 13:00 Add to calendar To be announced Selina Storm, EMBL Hamburg, Germany Host: Thomas Schneider
Seminar Room 48e, EMBL Hamburg
EMBL Distinguished Visitor Lecture
Thursday, 14 April 2016, 11:00 Add to calendar To be announced Erika L. Pearce, Max Planck Institute of Immunobiology and Epigenetics, Germany Host: Matthias Hentze
Large Operon, EMBL Heidelberg
EMBL Distinguished Visitor Lecture
Friday, 15 April 2016, 11:00 Add to calendar To be announced Mitchell Guttman, California Institute of Technology, USA Host: Sara Silva
EMBL Seminar Room
Hamburg Speaker
Friday, 15 April 2016, 13:00 Add to calendar To be announced Tuhin Bhowmick, EMBL Hamburg, Germany, Germany Seminar Room 48e, EMBL Hamburg
External Faculty Speaker
Monday, 18 April 2016, 11:00 Add to calendar To be announced Neil Brockdorff, Department of Biochemistry, University of Oxford, Oxford, United Kingdom Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
External Faculty Speaker
Friday, 22 April 2016, 11:00 Add to calendar The past, present and future of the pulvinar James A. Bourne, Australian Regenerative Medicine Institute, Monash University, Clayton, Australia Host: Cornelius Gross
CNR Seminar Room, EMBL Monterotondo
Hamburg Speaker
Friday, 22 April 2016, 13:00 Add to calendar To be announced Gleb Bourenkov, EMBL Hamburg, Germany Seminar Room 48e, EMBL Hamburg
EMBL Distinguished Visitor Lecture
Monday, 25 April 2016, 11:00 Add to calendar To be announced Dr. Alex Bortvin, Carnegie Institution for Science,Department of Embryology, Baltimore, Maryland, USA Host: Ramesh Pillai
EMBL Seminar Room, EMBL Grenoble
EMBL Distinguished Visitor Lecture
Friday, 29 April 2016, 10:00 Add to calendar Epigenetics and the determination of phenotype Emma Whitelaw, College of Science, Health and Engineering, School of Molecular Sciences, La Trobe Institute for Molecular Science, Melbourne (Bundoora), Australia Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
Abstract: Abstract:

I will discuss how the meaning of the word epigenetics has changed over the last ten years and why this has caused confusion. Empirical evidence has altered our view of the importance of DNA methylation in the determination of phenotype. My laboratory has just completed the analysis of a large ENU mutagenesis screen designed to identify proteins involved in epigenetic reprogramming. Around 50 mutant mouse lines were produced and the causative mutations found in most cases. These mutant mice have provided an opportunity to study the importance of these proteins in vivo.

Biography

Professor Emma Whitelaw is a molecular biologist working at the La Trobe Institute of Molecular Sciences, Melbourne. After completing her undergraduate degree at the Australian National University, she obtained a D.Phil at the University of Oxford and remained working in London and Oxford for the next fifteen years. In 1991, she joined the University of Sydney and focused her research on transcription. Her most notable research achievements are in the area of epigenetics. More recently she has extended her studies to include the interaction between the environment and the epigenome. In 2008 she was awarded an Australia Fellowship, the most prestigious fellowship available from the NHMRC, and in 2011 she became a Fellow of the Australian Academy of Science.
Hamburg Speaker
Friday, 29 April 2016, 13:00 Add to calendar To be announced Diana Freire, EMBL Hamburg, Germany Host: Matthias Wilmanns
Seminar Room 48e, EMBL Hamburg
External Faculty Speaker
Monday, 2 May 2016, 11:00 Add to calendar To be announced Claire Wyart, Institut du Cerveau et de la Moelle Épinière, , France Host: Yannick Schwab
Small Operon, EMBL Heidelberg
Tags: Cell Biology
Science and Society
Wednesday, 4 May 2016, 15:00 Add to calendar Experiments in democracy: why should we care about public engagement in science? James Wilsdon, Sheffield University, United Kingdom Host: Halldór Stefánsson
Large Operon, EMBL Heidelberg
EMBL Distinguished Visitor Lecture
Friday, 6 May 2016, 10:00 Add to calendar To be announced Qiufu Ma, Professor of Neurobiology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA, USA Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
EMBL Distinguished Visitor Lecture
Thursday, 12 May 2016, 11:00 Add to calendar To be announced Alessandro Costa, Francis Crick Institute, United Kingdom Host: Francesco Bisiak
EMBL Seminar Room, EMBL Grenoble
External Faculty Speaker
Friday, 13 May 2016, 11:00 Add to calendar Fear in the prey s mind Newton Canteras , Chair of Anatomy at the University of Sao Paulo, Sao Paulo, Brazil Host: Cornelius Gross
CNR Seminar Room, EMBL Monterotondo
EMBL Distinguished Visitor Lecture
Monday, 30 May 2016, 10:00 Add to calendar Transcriptional and Epigenetic Mechanisms of Depression Eric Nestler, Nash Family Professor and Chair, Department of Neuroscience Director, Friedman Brain Institute Icahn School of Medicine at Mount Sinai New York, NY, USA Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
Abstract: Depression is a common, chronic, and debilitating disease. Although many patients benefit from antidepressant medications or other therapies, only about half of depressed patients show a complete remission, which underscores the need for more effective agents. The mechanisms that precipitate depression, such as stress, are incompletely understood. One mystery of the disease is its long-lasting nature and delayed response to antidepressant treatment. This persistence is thought to be mediated by slowly developing but stable adaptations in the brain, which might include regulation of gene expression and chromatin structure.
We have used chronic social defeat stress as an animal model of depression that mimics certain symptoms of human depression. Prolonged exposure to an aggressor induces lasting changes in mouse behavior such as social avoidance, which are reversed by chronic (but not acute) treatment with available antidepressants. Importantly, roughly one-third of mice subjected to social defeat stress do not exhibit these deleterious behaviors and appear resilient. We are exploring the molecular basis of defeat-induced behavioral pathology, antidepressant action, and resilience by analyzing genome-wide changes in gene expression and chromatin modifications. Our work to date has focused on the nucleus accumbens, a key brain reward region implicated in aspects of depression, as well as several other limbic brain regions. Parallel work has focused on homologous regions in the brains of depressed humans examined postmortem.
Together, this work is providing novel insight into the molecular mechanisms underlying depression and other stress-related disorders. The findings also suggest novel leads for the development of new antidepressant treatments. For example, our findings on resilience suggest the novel approach of developing medications that promote resilience and not just those that oppose the deleterious effects of stress.

Biography

Dr. Nestler is the Nash Family Professor of Neuroscience at the Icahn School of Medicine at Mount Sinai in New York City, where he serves as Chair of the Department of Neuroscience and Director of the Friedman Brain Institute. He received his B.A., Ph.D., and M.D. degrees, and psychiatry residency training, from Yale University. He served on the Yale faculty from 1987-2000, where he was the Elizabeth Mears and House Jameson Professor of Psychiatry and Neurobiology, and Director of the Division of Molecular Psychiatry. He moved to Dallas in 2000 where he served as the Lou and Ellen McGinley Distinguished Professor and Chair of the Department of Psychiatry at The University of Texas Southwestern Medical Center until moving to New York in 2008. Dr. Nestler is a member of the Institute of Medicine and a Fellow of the American Academy of Arts and Sciences. He is a past President of the American College of Neuropsychopharmacology and President Elect of the Society for Neuroscience. The goal of Dr. Nestler s research is to better understand the molecular mechanisms of addiction and depression based on work in animal models, and to use this information to develop improved treatments of these disorders.
EMBL Distinguished Visitor Lecture
Thursday, 2 June 2016, 11:00 Add to calendar To be announced Timothy J. Mitchison, Harvard University, USA Host: François Nédélec
Large Operon, EMBL Heidelberg
EMBL Distinguished Visitor Lecture
Monday, 6 June 2016, 11:00 Add to calendar To be announced Phillipp Holliger, MRC-LMB Cambridge, United Kingdom Host: Lahari Yeramala
EMBL Seminar Room, EMBL Grenoble
EMBL Distinguished Visitor Lecture
Friday, 10 June 2016, 10:00 Add to calendar Making, Breaking and Linking Memories Sheena Josselyn, Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario, Canada Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
Abstract: Making, Breaking and Linking Memories .

A fundamental goal of neuroscience is to understand how information is encoded and stored in the brain. The physical or functional representation of a memory (the memory trace or engram ) is thought to be sparsely encoded over a distributed memory network. However, identifying the precise neurons which make up a memory trace has challenged for scientists since Karl Lashley s search for the engram in the 1950 s (Josselyn, 2015; Lashley, 1950; Josselyn, 2010; Josselyn et al., 2015). Moreover, it was not known why one neuron (rather than its neighbour) was involved in a given memory trace. We previously showed that lateral amygdala (LA) neurons with increased levels of the transcription factor CREB (cAMP/Ca++ Responsive Element Binding protein), are preferentially activated by fear memory expression, suggesting they are selectively recruited into the memory trace (Han et al., 2007). We, and others, went on to show that these neurons were critical components of the memory network by selectively ablating (Han et al., 2009) or inactivating them (Zhou et al., 2009). These findings established a causal link between a specific neuronal subpopulation and memory expression, thereby identifying critical neurons within the memory trace. Furthermore, these results suggest that at least within the LA, eligible neurons compete for inclusion in a memory trace, and that the winners of this competition are determined by relative CREB function. Although competition between neurons, axons and synapses is necessary for refining neural circuits in development, little is known about competition between neurons in the adult brain. Our recent results suggest that this neuronal competition during memory formation limits the overall size of the memory trace (number of winning neurons) and is a mechanism that links (or disambiguates) related memories in the LA.
Memory impairments are a hallmark of aging, major mental illnesses (e.g., schizophrenia and depression) as well as neurological disorders (e.g., Alzheimer's and Parkinson's diseases). Therefore, understanding how the brain encodes and stores information is highly relevant to both mental health and mental illness.

Biography

Sheena Josselyn is a Senior Scientist in the Neurosciences & Mental Health program at The Hospital for Sick Children (SickKids) and an Associate Professor in the departments of Psychology and Physiology and the Institute of Medical Sciences at the University of Toronto in Canada. She holds a Canada Research Chair in Molecular and Cellular Cognition and is an EJLB Scholar. Her undergraduate degrees and a Masters degree in Clinical Psychology were granted by Queen s University in Kingston. Sheena received a PhD in Neuroscience/Psychology from the University of Toronto with Dr. Franco Vaccarino as her supervisor. She conducted post-doctoral work with Dr. Mike Davis (Yale University) and Dr. Alcino Silva (UCLA). Her program of research is dedicated to understanding the neural basis of cognitive function and dysfunction. To unravel the molecular, cellular and circuit processes that underlie learning and memory, her lab uses a multidisciplinary approach that focuses on mouse models and attempts to translate these basic findings into humans.

Dr. Josselyn received the Innovations in Psychopharmacology Award from the Canadian College of Neuropsychopharmacology (CCNP) and the Effron Award from the American College of Neurospchycopharmacology (ACNP). She sits on the editorial board for the Neuropsychopharmacology, Journal of Neuroscience and the Neurobiology of Learning and Memory and serves on CIHR and NIH peer review panels

Science and Society
Monday, 27 June 2016, 11:00 Add to calendar To be announced Michael Eisen, Investigator, Howard Hughes Medical Institute, Professor of Genetics, Genomics and Development, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA Host: Shane Morley
CNR Seminar Room, EMBL Monterotondo
External Faculty Speaker
Thursday, 22 September 2016, 11:00 Add to calendar To be announced Dolf Weijers, Wageningen University, Netherlands Host: Marcus Heisler
Small Operon, EMBL Heidelberg
EMBL Distinguished Visitor Lecture
Friday, 23 September 2016, 10:00 Add to calendar Deciphering the physiology of hematopoiesis by fate mapping and endogenous barcoding Hans-Reimer Rodewald, Division of Cellular Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
Abstract: Abstract:

Information on the hematopoietic system has long relied on in vitro in colony assays and in vivo by transplantation of hematopoietic stem cells (HSC) into myeloablated recipients. Given that single cells can rebuild the blood and immune systems upon transplantation, HSC posses huge expansion potential (also coined self-renewal), and under these conditions single HSC are multipotent. Indeed, self-renewal and multipotency are often used as the key HSC-defining hallmarks, and it is commonly assumed that these properties also characterize HSC in situ. High throughput and other single cell technologies are currently being applied to study HSC properties but these approaches, too, may or may not reveal the functions of HSC under physiological conditions. To study the normal functions of HSC in the bone marrow under non-perturbed conditions, we have generated an in vivo experimental fate mapping system that allows tracking of the activity of HSC in situ under steady state conditions and post challenges. We quantified and modeled the cell fluxes through the hematopoietic system during its initial development and maintenance in adult mice, and obtained estimates on the numbers of HSC that contribute to adult hematopoiesis. In parallel, we are developing an endogenous Cre recombinase-dependent barcoding system that, again non-invasively, allows permanent genetic tagging of cells. We are currently characterizing the properties of this versatile tool to study cellular diversity and clonal dynamics in multicellular organs. By linking fate mapping with endogenous barcoding we aim at deciphering the physiology of hematopoiesis in vivo.


Short biography:
Hans-Reimer Rodewald is currently head of the Division for Cellular Immunology at the German Cancer Research Center in Heidelberg. The Rodewald laboratory has a long-standing interest in the development and function of the hematopoietic system. Work from this laboratory included the identification of lineage-committed progenitors, and essential cytokine signals in early T cell development. The Rodewald lab discovered thymus epithelial progenitor activity, leading to medullary epithelial islets, as a developmental mechanism of epithelial organogenesis, and identified the cervical thymus in the mouse. His laboratory developed mouse mutants, including specifically mast cell-deficient mice, to address open questions in mast cell biology, Recently, Rodewald and his colleagues uncovered the inbuilt property of the thymus for progenitor-independent thymus function (thymus autonomy), and noticed the pathological consequences of lack of cell competition in the thymus, i.e. T cell acute lymphoblastic leukemia. Current activities focus on the development of genetic tools to track stem cell output in vivo, aiming at deciphering the physiology of unperturbed hematopoiesis in vivo.
EMBL-La Sapienza Lecture
Friday, 7 October 2016, 11:00 Add to calendar To be announced Thalia Eley, Professor of Developmental Behavioural Genetics, Department of Social Genetic & Developmental Psychiatry, King's College London, London, United Kingdom Host: Cornelius Gross / Andrea Mele
Sapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma, EMBL Monterotondo
PSB Seminar
Tuesday, 11 October 2016, 16:00 Add to calendar To be announced Jenifer Doudna, University of California - Berkeley, USA Host: Ramesh Pillai
Chadwick Amphitheatre, Institute Laue Langevin, Grenoble
PSB Seminar
Wednesday, 12 October 2016, 16:00 Add to calendar To be announced Jenifer Doudna , University of California - Berkeley, USA Chadwick Amphitheatre, Institute Laue Langevin, Grenoble, EMBL Grenoble
External Faculty Speaker
Tuesday, 18 October 2016, 11:00 Add to calendar To be announced Cayetano Gonzalez, Barcelona Institute for Science and Technology, Spain Host: Anne Ephrussi
Small Operon, EMBL Heidelberg
EMBL Distinguished Visitor Lecture
Friday, 21 October 2016, 10:00 Add to calendar The First Steps in Vision: Cell types, Circuits and Repair Botond Roska, Friedrich Miescher Institute for Biomedical, Research, Basel, Switzerland Host: Philip Avner
CNR Seminar Room, EMBL Monterotondo
EMBL-La Sapienza Lecture
Friday, 28 October 2016, 11:00 Add to calendar To be announced Matteo Carandini, Institute of Ophthalmology, University College London, London, United Kingdom Host: Cornelius Gross / Andrea Mele
Sapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma, EMBL Monterotondo
EMBL-La Sapienza Lecture
Friday, 25 November 2016, 11:00 Add to calendar To be announced Adrian Bird, Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, Scotland, United Kingdom Host: Cornelius Gross / Irene Bozzoni
Sapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma, EMBL Monterotondo
EMBL-La Sapienza Lecture
Friday, 19 May 2017, 11:00 Add to calendar To be announced Jennifer Doudna, Li Ka Shing Chancellor's Chair in Biomedical and Health Sciences, Professor, Molecular & Cell Biology; Professor, Chemistry, University of California, Berkeley, CA, USA Host: Cornelius Gross / Irene Bozzoni
Sapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma, EMBL Monterotondo