Staff at EMBL

Biography

PhD 1998, postdoctoral research at the Cell Biology and
Metabolism Branch, NICHD, NIH, Bethesda, USA.
Group Leader at EMBL since 1999.
Head of Unit since 2006.

Publications

Chromatin structure and macromolecular confinement. Bancaud, Aurelien; Daigle, Nathalie; Mozziconacci, Julien; Ellenberg, Jan Biophysical Journal January 2007 14 A Suppl

Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. Bancaud, A., Huet, S., Daigle, N., Mozziconacci, J., Beaudouin, J. & Ellenberg, J. EMBO J. 2009 Dec 16;28(24):3785-98. Epub . PubMed

RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D.H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J. & Lukas, C. Cell. 2009 Feb 6;136(3):435-46. PubMed

Chromophore-Assisted Laser Inactivation of alpha- and gamma-Tubulin SNAP-tag Fusion Proteins inside Living Cells. Keppler, A. & Ellenberg, J. ACS Chem Biol. 2009 Feb 3. PubMed

A new model for asymmetric spindle positioning in mouse oocytes. Schuh, M. & Ellenberg, J. Curr Biol. 2008 Dec 23;18(24):1986-92. Epub 2008 Dec 8. PubMed

Sun1 forms immobile macromolecular assemblies at the nuclear envelope. Lu, W., Gotzmann, J., Sironi, L., Jaeger, V.M., Schneider, M., Luke, Y., Uhlen, M., Szigyarto, C.A., Brachner, A., Ellenberg, J., Foisner, R., Noegel, A.A. & Karakesisoglou, I. Biochim Biophys Acta. 2008 Dec;1783(12):2415-26. Epub 2008 Sep 19. PubMed

Nuclear pore complex assembly through the cell cycle: regulation and membrane organization. Antonin, W., Ellenberg, J. & Dultz, E. FEBS Lett. 2008 Jun 18;582(14):2004-16. Epub 2008 Mar 6. PubMed

Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells. Dultz, E., Zanin, E., Wurzenberger, C., Braun, M., Rabut, G., Sironi, L. & Ellenberg, J. J Cell Biol. 2008 Mar 10;180(5):857-65. Epub 2008 Mar 3. PubMed

Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes. Schuh, M. & Ellenberg, J. Cell. 2007 Aug 10;130(3):484-98. PubMed

lambda(N)-GFP: an RNA reporter system for live-cell imaging. Daigle, N. & Ellenberg, J. Nat Methods. 2007 Aug;4(8):633-6. Epub 2007 Jul 1. PubMed

Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinase. Mora-Bermudez, F., Gerlich, D. & Ellenberg, J. Nat Cell Biol. 2007 Jul;9(7):822-31. Epub 2007 Jun 10. PubMed

An RNAi screening platform to identify secretion machinery in mammalian cells. Simpson, J.C., Cetin, C., Erfle, H., Joggerst, B., Liebel, U., Ellenberg, J. & Pepperkok, R. J Biotechnol. 2007 Apr 30;129(2):352-65. Epub 2007 Jan 14. PubMed

Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit. Tarendeau, F., Boudet, J., Guilligay, D., Mas, P.J., Bougault, C.M., Boulo, S., Baudin, F., Ruigrok, R.W., Daigle, N., Ellenberg, J., Cusack, S., Simorre, J.P. & Hart, D.J. Nat Struct Mol Biol. 2007 Mar;14(3):229-33. Epub 2007 Feb 25. PubMed

Measuring structural dynamics of chromosomes in living cells by fluorescence microscopy. Mora-Bermudez, F. & Ellenberg, J. Methods. 2007 Feb;41(2):158-67. PubMed

Minimizing the risk of reporting false positives in large-scale RNAi screens. Echeverri, C.J., Beachy, P.A., Baum, B., Boutros, M., Buchholz, F., Chanda, S.K., Downward, J., Ellenberg, J., Fraser, A.G., Hacohen, N., Hahn, W.C., Jackson, A.L., Kiger, A., Linsley, P.S., Lum, L., Ma, Y., Mathey-Prevot, B., Root, D.E., Sabatini, D.M., Taipale, J., Perrimon, N. & Bernards, R. Nat Methods. 2006 Oct;3(10):777-9. PubMed

Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication. Gerlich, D., Koch, B., Dupeux, F., Peters, J.M. & Ellenberg, J. Curr Biol. 2006 Aug 8;16(15):1571-8. PubMed

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum. Keppler, A., Arrivoli, C., Sironi, L. & Ellenberg, J. Biotechniques. 2006 Aug;41(2):167-70, 172, 174-5. PubMed

Resolution of chiasmata in oocytes requires separase-mediated proteolysis. Kudo, N.R., Wassmann, K., Anger, M., Schuh, M., Wirth, K.G., Xu, H., Helmhart, W., Kudo, H., McKay, M., Maro, B., Ellenberg, J., de Boer, P. & Nasmyth, K. Cell. 2006 Jul 14;126(1):135-46. PubMed

NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubules. Ribbeck, K., Groen, A.C., Santarella, R., Bohnsack, M.T., Raemaekers, T., Kocher, T., Gentzel, M., Gorlich, D., Wilm, M., Carmeliet, G., Mitchison, T.J., Ellenberg, J., Hoenger, A. & Mattaj, I.W. Mol Biol Cell. 2006 Jun;17(6):2646-60. Epub 2006 Mar 29. PubMed

Nuclear actin: a lack of export allows formation of filaments. Schuh, M. & Ellenberg, J. Curr Biol. 2006 May 9;16(9):R321-3. PubMed

High-throughput RNAi screening by time-lapse imaging of live human cells. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J. Nat Methods. 2006 May;3(5):385-90. PubMed

Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins. Beaudouin, J., Mora-Bermudez, F., Klee, T., Daigle, N. & Ellenberg, J. Biophys J. 2006 Mar 15;90(6):1878-94. Epub 2005 Dec 30. PubMed

Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells. Gerlich, D., Hirota, T., Koch, B., Peters, J.M. & Ellenberg, J. Curr Biol. 2006 Feb 21;16(4):333-44. PubMed

Monitoring the permeability of the nuclear envelope during the cell cycle. Lenart, P. & Ellenberg, J. Methods 2006 Jan;38(1):17-24. PubMed

A contractile nuclear actin network drives chromosome congression in oocytes. Lenart, P., Bacher, C.P., Daigle, N., Hand, A.R., Eils, R., Terasaki, M. & Ellenberg, J. Nature 2005 Aug 11;436(7052):812-8. Epub 2005 Jul 13. PubMed

FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35. Chusainow, J., Ajuh, P.M., Trinkle-Mulcahy, L., Sleeman, J.E., Ellenberg, J. & Lamond, A.I. RNA 2005 Aug;11(8):1201-14. PubMed

Photobleaching techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, iFRAP and FLIP. Rabut, G. & Ellenberg, J. In "Live Cell Imaging: A Laboratory Manual Vol. 1", Goldman, R.D. and Spector, D.L., (Eds.), 101-127. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2005

Distinct functions of condensin I and II in mitotic chromosome assembly. Hirota, T., Gerlich, D., Koch, B., Ellenberg, J. & Peters, J.M. J Cell Sci 2004 Dec 15;117(Pt 26):6435-45. Epub 2004 Nov 30. PubMed

LAP2alpha and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly. Dechat, T., Gajewski, A., Korbei, B., Gerlich, D., Daigle, N., Haraguchi, T., Furukawa, K., Ellenberg, J. & Foisner, R. J Cell Sci 2004 Dec 1;117(Pt 25):6117-28. Epub 2004 Nov 16. PubMed

Mapping the dynamic organization of the nuclear pore complex inside single living cells. Rabut, G., Doye, V. & Ellenberg, J. Nat Cell Biol 2004 Nov;6(11):1114-21. Epub 2004 Oct 24. PubMed

Automatic real-time three-dimensional cell tracking by fluorescence microscopy. Rabut, G. & Ellenberg, J. J Microsc 2004 Nov;216(Pt 2):131-7. PubMed

Roles of polo-like kinase 1 in the assembly of functional mitotic spindles. Sumara, I., Gimenez-Abian, J.F., Gerlich, D., Hirota, T., Kraft, C., de la Torre, C., Ellenberg, J. & Peters, J.M. Curr Biol 2004 Oct 5;14(19):1712-22. PubMed

Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei. Bubulya, P.A., Prasanth, K.V., Deerinck, T.J., Gerlich, D., Beaudouin, J., Ellisman, M.H., Ellenberg, J. & Spector, D.L. J Cell Biol 2004 Oct 11;167(1):51-63. PubMed

Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells. Leung, A.K., Gerlich, D., Miller, G., Lyon, C., Lam, Y.W., Lleres, D., Daigle, N., Zomerdijk, J., Ellenberg, J. & Lamond, A.I. J Cell Biol 2004 Sep 13;166(6):787-800. Epub 2004 Sep 07. PubMed

Calcium rises locally trigger focal adhesion disassembly and enhance residency of focal adhesion kinase at focal adhesions. Giannone, G., Ronde, P., Gaire, M., Beaudouin, J., Haiech, J., Ellenberg, J. & Takeda, K. J Biol Chem 2004 Jul 2;279(27):28715-23. Epub 2004 Apr 21. PubMed

Regulation of sister chromatid cohesion between chromosome arms. Gimenez-Abian, J.F., Sumara, I., Hirota, T., Hauf, S., Gerlich, D., de la Torre, C., Ellenberg, J. & Peters, J.M. Curr Biol 2004 Jul 13;14(13):1187-93. PubMed

Dynamics of nuclear pore complex organization through the cell cycle. Rabut, G., Lenart, P. & Ellenberg, J. Curr Opin Cell Biol 2004 Jun;16(3):314-21. PubMed

Automatic identification of subcellular phenotypes on human cell arrays. Conrad, C., Erfle, H., Warnat, P., Daigle, N., Lorch, T., Ellenberg, J., Pepperkok, R. & Eils, R. Genome Res 2004 Jun;14(6):1130-6. PubMed

The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis. Loiodice, I., Alves, A., Rabut, G., Van Overbeek, M., Ellenberg, J., Sibarita, J.B. & Doye, V. Mol Biol Cell 2004 May 14;. PubMed

RanBP2/Nup358 Provides a Major Binding Site for NXF1-p15 Dimers at the Nuclear Pore Complex and Functions in Nuclear mRNA Export. Forler, D., Rabut, G., Ciccarelli, F.D., Herold, A., Kocher, T., Niggeweg, R., Bork, P., Ellenberg, J. & Izaurralde, E. Mol Cell Biol 2004 Feb;24(3):1155-67. PubMed

Light microscopy of echinoderm embryos. Strickland, L., von Dassow, G., Ellenberg, J., Foe, V., Lenart, P. & Burgess, D. Methods Cell Biol 2004;74:371-409. PubMed

NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization. Raemaekers, T., Ribbeck, K., Beaudouin, J., Annaert, W., Van Camp, M., Stockmans, I., Smets, N., Bouillon, R., Ellenberg, J. & Carmeliet, G. J Cell Biol 2003 Sep 15;162(6):1017-29. PubMed

4D imaging to assay complex dynamics in live specimens. Gerlich, D. & Ellenberg, J. Nat Cell Biol 2003 Sep;Suppl:S14-9. PubMed

Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes. Lénart, P., Rabut, G., Daigle, N., Hand, A.R., Terasaki, M. & Ellenberg, J. J Cell Biol 2003 Mar 31;160(7):1055-68. PubMed

Global chromosome positions are transmitted through mitosis in mammalian cells. Gerlich, D., Beaudouin, J., Kalbfuss, B., Daigle, N., Eils, R. & Ellenberg, J. Cell 2003 Mar 21;112(6):751-64. PubMed

Cyclic, proteasome-mediated turnover of unliganded and liganded ERalpha on responsive promoters is an integral feature of estrogen signaling. Reid, G., Hubner, M.R., Metivier, R., Brand, H., Denger, S., Manu, D., Beaudouin, J., Ellenberg, J. & Gannon, F. Mol Cell 2003 Mar;11(3):695-707. PubMed

Nuclear envelope dynamics in oocytes: from germinal vesicle breakdown to mitosis. Lenart, P. & Ellenberg, J. Curr Opin Cell Biol 2003 Feb;15(1):88-95. PubMed

Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p. Kruse, C., Jaedicke, A., Beaudouin, J., Bohl, F., Ferring, D., Guttler, T., Ellenberg, J. & Jansen, R.P. J Cell Biol 2002 Dec 23;159(6):971-82. PubMed

Chromosomal association of Ran during meiotic and mitotic divisions. Hinkle, B., Slepchenko, B., Rolls, M.M., Walther, T.C., Stein, P.A., Mehlmann, L.M., Ellenberg, J. & Terasaki, M. J Cell Sci 2002 Dec 1;115(Pt 23):4685-93. PubMed

Remodelling the walls of the nucleus. Burke, B. & Ellenberg, J. Nat Rev Mol Cell Biol 2002 Jul;3(7):487-97. PubMed

Histone H3 phosphorylation during Xenopus oocyte maturation: regulation by the MAP kinase/p90Rsk pathway and uncoupling from DNA condensation. Schmitt, A., Gutierrez, G.J., Lenart, P., Ellenberg, J. & Nebreda, A.R. FEBS Lett 2002 May 8;518(1-3):23-8. PubMed

Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Beaudouin, J., Gerlich, D., Daigle, N., Eils, R. & Ellenberg, J. Cell 2002 Jan 11;108(1):83-96. PubMed

Dynamics of nuclear envelope proteins during the cell cycle in mammalian cells. Ellenberg J. In "Dynamics of Nuclear Envelope Assembly in Embryos and Somatic Cells, Vol. 23", P. Collas (ed), 18-25. Landes Biosciences, Georgetown, Texas, USA, 2002.

An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. Belgareh, N., Rabut, G., Bai, S.W., van Overbeek, M., Beaudouin, J., Daigle, N., Zatsepina, O.V., Pasteau, F., Labas, V., Fromont-Racine, M., Ellenberg, J. & Doye, V. J Cell Biol 2001 Sep 17;154(6):1147-60. PubMed

Four-dimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells. Gerlich, D., Beaudouin, J., Gebhard, M., Ellenberg, J. & Eils, R. Nat Cell Biol 2001 Sep;3(9):852-5. PubMed

Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. Daigle, N., Beaudouin, J., Hartnell, L., Imreh, G., Hallberg, E., Lippincott-Schwartz, J. & Ellenberg, J. J Cell Biol 2001 Jul 9;154(1):71-84. PubMed

Nucleocytoplasmic transport: diffusion channel or phase transition? Rabut, G. & Ellenberg, J. Curr Biol 2001 Jul 24;11(14):R551-4. PubMed

A new model for nuclear envelope breakdown. Terasaki, M., Campagnola, P., Rolls, M.M., Stein, P.A., Ellenberg, J., Hinkle, B. & Slepchenko, B. Mol Biol Cell 2001 Feb;12(2):503-10. PubMed

A bromodomain protein, MCAP, associates with mitotic chromosomes and affects G(2)-to-M transition. Dey, A., Ellenberg, J., Farina, A., Coleman, A.E., Maruyama, T., Sciortino, S., Lippincott-Schwartz, J. & Ozato, K. Mol Cell Biol 2000 Sep;20(17):6537-49 PubMed

Dynamics and retention of misfolded proteins in native ER membranes Nehls, S., Snapp, E.L., Cole, N.B., Zaal, K.J., Kenworthy, A.K., Roberts, T.H., Ellenberg, J., Presley, J.F., Siggia, E. & Lippincott-Schwartz, J. Nat Cell Biol 2000 May;2(5):288-95 PubMed

Golgi membranes are absorbed into and reemerge from the ER during mitosis Zaal, K.J., Smith, C.L., Polishchuk, R.S., Altan, N., Cole, N.B., Ellenberg, J., Hirschberg, K., Presley, J.F., Roberts, T.H., Siggia, E., Phair, R.D. & Lippincott-Schwartz, J. Cell 1999 Dec 10;99(6):589-601 PubMed

Dynamics and mobility of nuclear envelope proteins in interphase and mitotic cells revealed by green fluorescent protein chimeras. Ellenberg, J. & Lippincott-Schwartz, J. Methods 1999 Nov;19(3):362-72 PubMed

Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein. Ostlund, C., Ellenberg, J., Hallberg, E., Lippincott-Schwartz, J. & Worman, H.J. J Cell Sci 1999 Jun;112 ( Pt 11):1709-19 PubMed

Dual-colour imaging with GFP variants. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J.F. Trends Cell Biol 1999 Feb;9(2):52-6 PubMed

Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein. Lippincott-Schwartz, J., Presley, J.F., Zaal, K.J., Hirschberg, K., Miller, C.D. & Ellenberg, J. Methods Cell Biol 1999;58:261-81 PubMed

Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells. Hirschberg, K., Miller, C.M., Ellenberg, J., Presley, J.F., Siggia, E.D., Phair, R.D. & Lippincott-Schwartz, J. J Cell Biol 1998 Dec 14;143(6):1485-503 PubMed

ZAP-70 association with T cell receptor zeta (TCRzeta): fluorescence imaging of dynamic changes upon cellular stimulation. Sloan-Lancaster, J., Presley, J., Ellenberg, J., Yamazaki, T., Lippincott-Schwartz, J. & Samelson, L.E. J Cell Biol 1998 Nov 2;143(3):613-24 PubMed

Two-color green fluorescent protein time-lapse imaging. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J.F. Biotechniques 1998 Nov;25(5):838-42, 844-6 PubMed

Retrograde transport of Golgi-localized proteins to the ER. Cole, N.B., Ellenberg, J., Song, J., DiEuliis, D. & Lippincott-Schwartz, J. J Cell Biol 1998 Jan 12;140(1):1-15 PubMed

Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. Ellenberg, J., Siggia, E.D., Moreira, J.E., Smith, C.L., Presley, J.F., Worman, H.J. & Lippincott-Schwartz, J. J Cell Biol 1997 Sep 22;138(6):1193-206 PubMed

The transmembrane domain of a carboxyl-terminal anchored protein determines localization to the endoplasmic reticulum. Yang, M., Ellenberg, J., Bonifacino, J.S. & Weissman, A.M. J Biol Chem 1997 Jan 17;272(3):1970-5 PubMed

Fluorescence photobleaching techniques. Ellenberg, J. & Lippincott-Schwartz, J. In "Cells: A Laboratory Manual, Vol. 2", Spector, D., Goldman, R. & Leinwand, L. (Eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1997, 79.1-79.23