Staff at EMBL

Dr. Rainer Pepperkok

Team Leader, Head of Advanced Light Microscopy Core Facility and Senior Scientist
Pepperkok Team Advanced Light Microscopy Facility

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

E-mail:	send a mail

Biography

PhD 1992, University Kaiserslautern.
Postdoctoral research at University of Geneva.
Lab head at the Imperial Cancer Research Fund, London.
At EMBL since 1998.
Senior scientist since 2012.

Publications

Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding. Iskar, M., Zeller, G., Blattmann, P., Campillos, M., Kuhn, M., Kaminska, K.H., Runz, H., Gavin, A.C., Pepperkok, R., van Noort, V. & Bork, P. Mol Syst Biol. 2013 Apr 30;9:662. doi: 10.1038/msb.2013.20. PubMed

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Stadler, C., Rexhepaj, E., Singan, V.R., Murphy, R.F., Pepperkok, R., Uhlen, M., Simpson, J.C. & Lundberg, E. Nat Methods. 2013 Apr;10(4):315-23. doi: 10.1038/nmeth.2377. Epub 2013 Feb 24. PubMed

RNAi-Based Functional Profiling of Loci from Blood Lipid Genome-Wide Association Studies Identifies Genes with Cholesterol-Regulatory Function. Blattmann, P., Schuberth, C., Pepperkok, R. & Runz, H. PLoS Genet. 2013 Feb;9(2):e1003338. doi: 10.1371/journal.pgen.1003338. Epub 2013Feb 28. PubMed

Cell-to-cell communication: current views and future perspectives. Gerdes, H.H. & Pepperkok, R. Cell Tissue Res. 2013 Apr;352(1):1-3. doi: 10.1007/s00441-013-1590-1. Epub 2013Feb 26. PubMed

Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia A. Roth, S.D., Schuttrumpf, J., Milanov, P., Abriss, D., Ungerer, C., Quade-Lyssy, P., Simpson, J.C., Pepperkok, R., Seifried, E. & Tonn, T. PLoS One. 2012;7(9):e44505. doi: 10.1371/journal.pone.0044505. Epub 2012 Sep 4. PubMed

Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway. Simpson, J.C., Joggerst, B., Laketa, V., Verissimo, F., Cetin, C., Erfle, H., Bexiga, M.G., Singan, V.R., Heriche, J.K., Neumann, B., Mateos, A., Blake, J., Bechtel, S., Benes, V., Wiemann, S., Ellenberg, J. & Pepperkok, R. Nat Cell Biol. 2012 Jun 3;14(7):764-74. doi: 10.1038/ncb2510. PubMed

Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1. Moudry, P., Lukas, C., Macurek, L., Neumann, B., Heriche, J.K., Pepperkok, R., Ellenberg, J., Hodny, Z., Lukas, J. & Bartek, J. Cell Death Differ. 2012 May;19(5):798-807. doi: 10.1038/cdd.2011.150. Epub 2011Nov 11. PubMed

A new approach to manipulate the fate of single neural stem cells in tissue. Taverna, E., Haffner, C., Pepperkok, R. & Huttner, W.B. Nat Neurosci. 2011 Dec 18;15(2):329-37. doi: 10.1038/nn.3008. PubMed

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. Stadler, C., Hjelmare, M., Neumann, B., Jonasson, K., Pepperkok, R., Uhlen, M. & Lundberg, E. J Proteomics. 2012 Feb 15. PubMed

Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Contreras, F.X., Ernst, A.M., Haberkant, P., Bjorkholm, P., Lindahl, E., Gonen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F. & Brugger, B. Nature. 2012 Jan 9;481(7382):525-9. doi: 10.1038/nature10742. PubMed

At the cutting edge: applications and perspectives of laser nanosurgery in cell biology. Ronchi, P., Terjung, S. & Pepperkok, R. Biol Chem. 2012 Apr;393(4):235-48. doi: 10.1515/hsz-2011-0237. PubMed

Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching--based fluorescence resonance energy transfer measurements Seitz, A., Terjung, S., Zimmermann, T. and Pepperkok, R. J. Biomed. Opt. 17, 011010 (2012), DOI:10.1117/1.JBO.17.1.011010

Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Ritzerfeld, J., Remmele, S., Wang, T., Temmerman, K., Brugger, B., Wegehingel, S., Tournaviti, S., Strating, J.R., Wieland, F.T., Neumann, B., Ellenberg, J., Lawerenz, C., Hesser, J., Erfle, H., Pepperkok, R. & Nickel, W. Genome Res. 2011 Nov;21(11):1955-68. doi: 10.1101/gr.116087.110. Epub 2011 Jul27. PubMed

Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation. Moore, H.M., Bai, B., Boisvert, F.M., Latonen, L., Rantanen, V., Simpson, J.C., Pepperkok, R., Lamond, A.I. & Laiho, M. Mol Cell Proteomics. 2011 Oct;10(10):M111.009241. Epub 2011 Jul 21. PubMed

A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells. Tangemo, C., Ronchi, P., Colombelli, J., Haselmann, U., Simpson, J.C., Antony, C., Stelzer, E.H., Pepperkok, R. & Reynaud, E.G. J Cell Sci. 2011 Mar 15;124(Pt 6):978-87. doi: 10.1242/jcs.079640. PubMed

Micropilot: automation of fluorescence microscopy-based imaging for systems biology. Conrad, C., Wunsche, A., Tan, T.H., Bulkescher, J., Sieckmann, F., Verissimo, F., Edelstein, A., Walter, T., Liebel, U., Pepperkok, R. & Ellenberg, J. Nat Methods. 2011 Mar;8(3):246-9. doi: 10.1038/nmeth.1558. Epub 2011 Jan 23. PubMed

Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. Reiss, S., Rebhan, I., Backes, P., Romero-Brey, I., Erfle, H., Matula, P., Kaderali, L., Poenisch, M., Blankenburg, H., Hiet, M.S., Longerich, T., Diehl, S., Ramirez, F., Balla, T., Rohr, K., Kaul, A., Buhler, S., Pepperkok, R., Lengauer, T., Albrecht, M., Eils, R., Schirmacher, P., Lohmann, V. & Bartenschlager, R. Cell Host Microbe. 2011 Jan 20;9(1):32-45. doi: 10.1016/j.chom.2010.12.002. PubMed

Functional genomics assays to study CFTR traffic and ENaC function. Almaca, J., Dahimene, S., Appel, N., Conrad, C., Kunzelmann, K., Pepperkok, R. & Amaral, M.D. Methods Mol Biol. 2011;742:249-64. doi: 10.1007/978-1-61779-120-8_15. PubMed

In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays. Grecco, H.E., Roda-Navarro, P., Girod, A., Hou, J., Frahm, T., Truxius, D.C., Pepperkok, R., Squire, A. & Bastiaens, P.I. Nat Methods. 2010 Jun;7(6):467-72. doi: 10.1038/nmeth.1458. Epub 2010 May 9. PubMed

Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells. Subramanian, D.*, Laketa, V.*, Muller, R., Tischer, C., Zarbakhsh, S., Pepperkok, R. & Schultz, C. * - equally contributing first authors. Nat Chem Biol. 2010 May;6(5):324-6. Epub 2010 Apr 4. PubMed

Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging. Walter, T., Held, M., Neumann, B., Heriche, J.K., Conrad, C., Pepperkok, R. & Ellenberg, J. J Struct Biol. 2010 Apr;170(1):1-9. doi: 10.1016/j.jsb.2009.10.004. Epub 2009 Oct23. PubMed

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J. Nature. 2010 Apr 1;464(7289):721-7. PubMed

High-Throughput Microscopy Using Live Mammalian Cells. Terjung, S., Walter, T., Seitz, A., Neumann, B., Pepperkok, R. & Ellenberg, J. In 'Live Cell Imaging: A Laboratory Manual', Second Edition, 2010 (eds. R.D. Goldman, J.R. Swedlow & D.L. Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor

Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth. Laketa, V., Zarbakhsh, S., Morbier, E., Subramanian, D., Dinkel, C., Brumbaugh, J., Zimmermann, P., Pepperkok, R. & Schultz, C. Chem Biol. 2009 Nov 25;16(11):1190-6. PubMed

Factor VIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of factor VIII. Heinz, S., Schuttrumpf, J., Simpson, J.C., Pepperkok, R., Nicolaes, G.A., Abriss, D., Milanov, P., Roth, S., Seifried, E. & Tonn, T. Thromb Haemost. 2009 Nov;102(5):925-35. PubMed

Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins. Tournaviti, S., Pietro, E.S., Terjung, S., Schafmeier, T., Wegehingel, S., Ritzerfeld, J., Schulz, J., Smith, D.F., Pepperkok, R. & Nickel, W. Traffic. 2009 Aug;10(8):1047-60. Epub 2009 May 12. PubMed

Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II. Schwarz, K., Iolascon, A., Verissimo, F., Trede, N.S., Horsley, W., Chen, W., Paw, B.H., Hopfner, K.P., Holzmann, K., Russo, R., Esposito, M.R., Spano, D., De Falco, L., Heinrich, K., Joggerst, B., Rojewski, M.T., Perrotta, S., Denecke, J., Pannicke, U., Delaunay, J., Pepperkok, R. & Heimpel, H. Nat Genet. 2009 Aug;41(8):936-40. Epub 2009 Jun 28. PubMed

DetecTiff(C): A Novel Image Analysis Routine for High-Content Screening Microscopy. Gilbert, D.F., Meinhof, T., Pepperkok, R. & Runz, H. J Biomol Screen. 2009 Jul 29. PubMed

ojoplano-mediated basal constriction is essential for optic cup morphogenesis. Martinez-Morales, J.R., Rembold, M., Greger, K., Simpson, J.C., Brown, K.E., Quiring, R., Pepperkok, R., Martin-Bermudo, M.D., Himmelbauer, H. & Wittbrodt, J. Development. 2009 Jul;136(13):2165-75. PubMed

Identification of cholesterol-regulating genes by targeted RNAi screening. Bartz, F., Kern, L., Erz, D., Zhu, M., Gilbert, D., Meinhof, T., Wirkner, U., Erfle, H., Muckenthaler, M., Pepperkok, R. & Runz, H. Cell Metab. 2009 Jul;10(1):63-75. PubMed

RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D.H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J. & Lukas, C. Cell. 2009 Feb 6;136(3):435-46. PubMed

Rab18 and Rab43 have key roles in ER-Golgi trafficking Dejgaard, SY; Murshid, A; Erman, A; Kizilay, O; Verbich, D; Lodge, R; Dejgaard, K; Ly-Hartig, TBN; Pepperkok, R.; Simpson, JC; Presley, JF JOURNAL OF CELL SCIENCE 121 (16): 2768-2781 AUG 15 2008 PubMed

Rab18 and Rab43 have key roles in ER-Golgi trafficking. Dejgaard, S.Y., Murshid, A., Erman, A., Kizilay, O., Verbich, D., Lodge, R., Dejgaard, K., Ly-Hartig, T.B., Pepperkok, R., Simpson, J.C. & Presley, J.F. J Cell Sci. 2008 Aug 15;121(Pt 16):2768-81. Epub 2008 Jul 29. PubMed

Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. Erfle, H., Neumann, B., Rogers, P., Bulkescher, J., Ellenberg, J. & Pepperkok, R. J Biomol Screen. 2008 Aug;13(7):575-80. Epub 2008 Jul 3. PubMed

In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum. Pouthas, F., Girard, P., Lecaudey, V., Ly, T.B., Gilmour, D., Boulin, C., Pepperkok, R. & Reynaud, E.G. J Cell Sci. 2008 Jul 15;121(Pt 14):2406-14. Epub 2008 Jun 24. PubMed

Integrating systems biology with clinical research. Pepperkok, R. & Wiemann, S. Genome Biol. 2008;9(7):314. Epub 2008 Jul 1. PubMed

A correlative light and electron microscopy method based on laser micropatterning and etching. Colombelli J, Tängemo C, Haselmann U, Antony, C., Stelzer, E.H.K., Pepperkok, R., Reynaud EG Methods in Molecular Biology, Vol. 457: Membrane Trafficking, A. Vancura (ed), Humana Press, 2008, p.203-214

Transfected cell microarrays: an efficient tool for high-throughput functional analysis. Starkuviene, V., Pepperkok, R. & Erfle, H. Expert Rev Proteomics. 2007 Aug;4(4):479-89. PubMed

The potential of high-content high-throughput microscopy in drug discovery. Starkuviene, V. & Pepperkok, R. Br J Pharmacol. 2007 Jul 2;. PubMed

Differential Requirements for ts-O45-G and Procollagen Biosynthetic Transport. Starkuviene, V. & Pepperkok, R. Traffic. 2007 Jun 5;. PubMed

Maturation of the mammalian secretome. Simpson, J.C., Mateos, A. & Pepperkok, R. Genome Biol. 2007 Apr 30;8(4):211. PubMed

An RNAi screening platform to identify secretion machinery in mammalian cells. Simpson, J.C., Cetin, C., Erfle, H., Joggerst, B., Liebel, U., Ellenberg, J. & Pepperkok, R. J Biotechnol. 2007 Apr 30;129(2):352-65. Epub 2007 Jan 14. PubMed

High-content microscopy identifies new neurite outgrowth regulators. Laketa, V., Simpson, J.C., Bechtel, S., Wiemann, S. & Pepperkok, R. Mol Biol Cell. 2007 Jan;18(1):242-52. Epub 2006 Nov 8. PubMed

Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. Grabher, C., Cliffe, A., Miura, K., Pepperkok, R., Rorth, P. & Wittbrodt, J. J Leukoc Biol. 2007 Jan;81(1):263-71. PubMed

Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome. von Brunn, A., Teepe, C., Simpson, J.C., Pepperkok, R., Friedel, C.C., Zimmer, R., Roberts, R., Baric, R. & Haas, J. PLoS ONE. 2007 May 23;2(5):e459. PubMed

Membrane dynamics in the early secretory pathway. Robinson, DG; Herranz, MC; Bubeck, J; Pepperkok, R.; Ritzenthaler, C Critical Reviews in Plant Sciences 2007 26(4)

Reverse transfection on cell arrays for high content screening microscopy. Erfle, H., Neumann, B., Liebel, U., Rogers, P., Held, M., Walter, T., Ellenberg, J. & Pepperkok, R. Nat Protoc. 2007;2(2):392-9. PubMed

A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex. Stauber, T., Simpson, J.C., Pepperkok, R. & Vernos, I. Curr Biol. 2006 Nov 21;16(22):2245-51. PubMed

Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion. Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. & Pepperkok, R. Traffic. 2006 Sep;7(9):1283-9. PubMed

Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway. Verissimo, F., Silva, E., Morris, J.D., Pepperkok, R. & Jordan, P. Oncogene. 2006 Jul 13;25(30):4172-82. Epub 2006 Feb 27. PubMed

Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G. Runz, H., Miura, K., Weiss, M. & Pepperkok, R. EMBO J. 2006 Jul 12;25(13):2953-65. Epub 2006 Jun 22. PubMed

Laser nanosurgery in cell biology Colombelli, J., Pepperkok, R., Stelzer, E.H.K. & Reynaud, E.G. Med Sci (Paris). 2006 Jul-Aug;22(6-7):651-8. PubMed

Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells. Tabary, O., Boncoeur, E., de Martin, R., Pepperkok, R., Clement, A., Schultz, C. & Jacquot, J. Cell Signal. 2006 May;18(5):652-60. Epub 2005 Aug 9. PubMed

High-throughput RNAi screening by time-lapse imaging of live human cells. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J. Nat Methods. 2006 May;3(5):385-90. PubMed

Biogenesis of tubular ER-to-Golgi transport intermediates. Simpson, J.C., Nilsson, T. & Pepperkok, R. Mol Biol Cell. 2006 Feb;17(2):723-37. Epub 2005 Nov 28. PubMed

Secretory cargo regulates the turnover of COPII subunits at single ER exit sites. Forster, R., Weiss, M., Zimmermann, T., Reynaud, E.G., Verissimo, F., Stephens, D.J. & Pepperkok, R. Curr Biol. 2006 Jan 24;16(2):173-9. PubMed

The subcellular localization of the mammalian proteome comes a fraction closer. Simpson, J.C. & Pepperkok, R. Genome Biol. 2006;7(6):222. PubMed

The LIFEdb database in 2006. Mehrle, A., Rosenfelder, H., Schupp, I., del Val, C., Arlt, D., Hahne, F., Bechtel, S., Simpson, J., Hofmann, O., Hide, W., Glatting, K.H., Huber, W., Pepperkok, R., Poustka, A. & Wiemann, S. Nucleic Acids Res. 2006 Jan 1;34(Database issue):D415-8. PubMed

Production of siRNA- and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells. Erfle, H. & Pepperkok, R. Methods Mol Biol. 2006;360:155-62. PubMed

In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Colombelli, J., Reynaud, E.G., Rietdorf, J., Pepperkok, R. & Stelzer, E.H.K. Traffic. 2005 Dec;6(12):1093-102. PubMed

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization. Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B., Knop, M., Scheffzek, K. & Pepperkok, R. BMC Biol. 2005 Oct 7;3:21. PubMed

IR micro spectroscopy of live cells. Moss, D.A., Keese, M., Pepperkok, R. Vibrational Spectroscopy 2005 JUL;38(1-2): 185-191.

Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues. Keese, M., Magdeburg, R.J., Herzog, T., Hasenberg, T., Offterdinger, M., Pepperkok, R., Sturm, J.W. & Bastiaens, P.I. J Biol Chem 2005 Jul 29;280(30):27826-31. Epub 2005 May 20. PubMed

Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions. Knauer, S.K., Moodt, S., Berg, T., Liebel, U., Pepperkok, R. & Stauber, R.H. Traffic 2005 Jul;6(7):594-606. PubMed

Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Yang, Y.D., Elamawi, R., Bubeck, J., Pepperkok, R., Ritzenthaler, C. & Robinson, D.G. Plant Cell 2005 May;17(5):1513-31. Epub 2005 Apr 1. PubMed

Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking. Neubrand, V.E., Will, R.D., Mobius, W., Poustka, A., Wiemann, S., Schu, P., Dotti, C.G., Pepperkok, R. & Simpson, J.C. EMBO J 2005 Mar 23;24(6):1122-33. Epub 2005 Mar 10. PubMed

Live imaging of bidirectional traffic from the ERGIC. Ben-Tekaya, H., Miura, K., Pepperkok, R. & Hauri, H.P. J Cell Sci 2005 Jan 15;118(Pt 2):357-67. Epub 2005 Jan 04. PubMed

Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'. Young, J., Stauber, T., del Nery, E., Vernos, I., Pepperkok, R. & Nilsson, T. Mol Biol Cell 2005 Jan;16(1):162-77. Epub 2004 Oct 13. PubMed

Coupling of ER exit to microtubules through direct interaction of COPII with dynactin. Watson, P., Forster, R., Palmer, K.J., Pepperkok, R. & Stephens, D.J. Nat Cell Biol 2005 Jan;7(1):48-55. Epub 2004 Dec 5. PubMed

EAMNET - a resource and teaching framework for advanced light microscopy. Terjung, S., Pepperkok, R., Ankerhold, R., Caiolfa, V.R., Castel, S., Majer, P., Geiger, B., Luini, A., Salamero, J., Shorte, S. & Small, J.V. European Journal of Cell Biology 2005 84 108

Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources. Liebel, U., Kindler, B. & Pepperkok, R. Methods Enzymol. 2005;404:19-26. PubMed

Arrays of transfected mammalian cells for high content screening microscopy. Erfle, H. & Pepperkok, R. Methods Enzymol. 2005;404:1-8. PubMed

Novel cancer relevant cell cycle modulators identified in automated cell-based assays Dorit Arlt, Huber, W., Mamatha Sauermann, Meher Majety, Florian Hahne, Pepperkok, R., Annemarie Poustka, and Stefan Wiemann European Journal of Cell Biology, 84(Suppl. 55):30, 2005

Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle. Arlt, D., Huber, W., Liebel, U., Schmidt, C., Majety, M., Sauermann, M., Rosenfelder, H., Bechtel, S., Mehrle, A., Bannasch, D., Schupp, I., Seiler, M., Simpson, J.C., Hahne, F., Moosmayer, P., Ruschhaupt, M., Guilleaume, B., Wellenreuther, R., Pepperkok, R., Sultmann, H., Poustka, A. & Wiemann, S. Cancer Res 2005 Sep 1;65(17):7733-42. PubMed

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts. Brunet, S., Sardon, T., Zimmerman, T., Wittmann, T., Pepperkok, R., Karsenti, E. & Vernos, I. Mol Biol Cell 2004 Dec;15(12):5318-28. Epub . PubMed

From ORFeome to biology: a functional genomics pipeline. Wiemann, S., Arlt, D., Huber, W., Wellenreuther, R., Schleeger, S., Mehrle, A., Bechtel, S., Sauermann, M., Korf, U., Pepperkok, R., Sultmann, H. & Poustka, A. Genome Res. 2004 Oct;14(10B):2136-44. PubMed

High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Starkuviene, V., Liebel, U., Simpson, J.C., Erfle, H., Poustka, A., Wiemann, S. & Pepperkok, R. Genome Res 2004 Oct;14(10A):1948-56. PubMed

siRNA cell arrays for high-content screening microscopy. Erfle, H., Simpson, J.C., Bastiaens, P.I. & Pepperkok, R. Biotechniques 2004 Sep;37(3):454-8, 460, 462. PubMed

'Harvester': a fast meta search engine of human protein resources. Liebel, U., Kindler, B. & Pepperkok, R. Bioinformatics 2004 Aug 12;20(12):1962-3. Epub 2004 Feb 26. PubMed

Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum. Stephens, D.J. & Pepperkok, R. J Cell Sci 2004 Jul 15;117(Pt 16):3635-44. PubMed

Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells. Becker, S., Simpson, J.C., Pepperkok, R., Heinz, S., Herder, C., Grez, M., Seifried, E. & Tonn, T. Thromb Haemost 2004 Jul;92(1):23-35. PubMed

Automatic identification of subcellular phenotypes on human cell arrays. Conrad, C., Erfle, H., Warnat, P., Daigle, N., Lorch, T., Ellenberg, J., Pepperkok, R. & Eils, R. Genome Res 2004 Jun;14(6):1130-6. PubMed

LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system. Bannasch, D., Mehrle, A., Glatting, K.H., Pepperkok, R., Poustka, A. & Wiemann, S. Nucleic Acids Res 2004 Jan 1;32 Database issue:D505-8. PubMed

A microscope-based screening platform for large-scale functional protein analysis in intact cells. Liebel, U., Starkuviene, V., Erfle, H., Simpson, J.C., Poustka, A., Wiemann, S. & Pepperkok, R. FEBS Lett 2003 Nov 20;554(3):394-8. PubMed

Spectral imaging and its applications in live cell microscopy. Zimmermann, T., Rietdorf, J. & Pepperkok, R. FEBS Lett 2003 Jul 3;546(1):87-92. PubMed

The German cDNA network: cDNAs, functional genomics and proteomics. Wiemann, S., Bechtel, S., Bannasch, D., Pepperkok, R. & Poustka, A. J Struct Funct Genomics 2003;4(2-3):87-96. PubMed

Localizing the proteome. Simpson, J.C. & Pepperkok, R. Genome Biol 2003;4(12):240. Epub 2003 Nov 18. PubMed

Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. Zimmermann, T., Rietdorf, J., Girod, A., Georget, V. & Pepperkok, R. FEBS Lett 2002 Nov 6;531(2):245-9. PubMed

Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Gruss, O.J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., Karsenti, E., Mattaj, I.W. & Vernos, I. Nat Cell Biol 2002 Nov;4(11):871-9. PubMed

Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection. Richards, A.A., Stang, E., Pepperkok, R. & Parton, R.G. Mol Biol Cell 2002 May;13(5):1750-64. PubMed

Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells. Stephens, D.J. & Pepperkok, R. J Cell Sci 2002 Mar 15;115(Pt 6):1149-60. PubMed

Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells. Runz, H., Rietdorf, J., Tomic, I., de Bernard, M., Beyreuther, K., Pepperkok, R. & Hartmann, T. J Neurosci 2002 Mar 1;22(5):1679-89. PubMed

A fully automated high content screening system for cell based genome analyses. Liebel, U.& Pepperkok, R. Transkript Laborwelt 2002 1 4-8

The Spir actin organizers are involved in vesicle transport processes. Kerkhoff, E., Simpson, J.C., Leberfinger, C.B., Otto, I.M., Doerks, T., Bork, P., Rapp, U.R., Raabe, T. & Pepperkok, R. Curr Biol 2001 Dec 11;11(24):1963-8. PubMed

The many ways to cross the plasma membrane. Stephens, D.J. & Pepperkok, R. Proc Natl Acad Sci U S A 2001 Apr 10;98(8):4295-8. PubMed

Illuminating the secretory pathway: when do we need vesicles? Stephens, D.J. & Pepperkok, R. J Cell Sci 2001 Mar;114(Pt 6):1053-9. PubMed

Illuminating the human genome. Simpson, J.C., Neubrand, V.E., Wiemann, S. & Pepperkok, R. Histochem Cell Biol 2001 Jan;115(1):23-9. PubMed

Being in the right location at the right time. Pepperkok, R., Simpson, J.C. & Wiemann, S. Genome Biol 2001;2(9):REVIEWS1024. PubMed

Observing proteins in their natural habitat: the living cell. Bastiaens, P.I. & Pepperkok, R. Trends Biochem Sci 2000 Dec 1;25(12):631-637 PubMed

Breaking the COPI monopoly on Golgi recycling. Storrie, B., Pepperkok, R. & Nilsson, T. Trends Cell Biol. 2000 Sep;10(9):385-91. PubMed

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. Simpson, J.C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S. EMBO rep 2000 Sep;1(3):287-92. PubMed

COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. Stephens, D.J., Lin-Marq, N., Pagano, A., Pepperkok, R. & Paccaud, J.P. J Cell Sci 2000 Jun;113 ( Pt 12):2177-85. PubMed

Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation. Pepperkok, R., Hotz-Wagenblatt, A., Konig, N., Girod, A., Bossemeyer, D. & Kinzel, V. J Cell Biol 2000 Feb 21;148(4):715-26 PubMed

The p24 family member p23 is required for early embryonic development. Denzel, A., Otto, F., Girod, A., Pepperkok, R., Watson, R., Rosewell, I., Bergeron, J.J., Solari, R.C. & Owen, M.J. Curr Biol 2000 Jan 13;10(1):55-8 PubMed

COPI vesicles accumulating in the presence of a GTP restricted Arf1 mutant are depleted of anterograde and retrograde cargo. Pepperkok, R., Whitney, J.A., Gomez, M. & Kreis, T.E. J Cell Sci 2000;113(Pt 1):135-144 PubMed

Fluorescence microscopy of living vertebrate cells. Pepperkok, R., & Shima, G. In "Protein Localisation by Fluorescence Microscopy", V.J. Allan (ed.), Oxford Univ. Press, pp. 109-132

Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita. Heiss, N.S., Girod, A., Salowsky, R., Wiemann, S., Pepperkok, R. & Poustka, A. Hum Mol Genet 1999 Dec;8(13):2515-24 PubMed

Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum. Girod, A., Storrie, B., Simpson, J.C., Johannes, L., Goud, B., Roberts, L.M., Lord, J.M., Nilsson, T. & Pepperkok, R. Nat Cell Biolog 1999 Nov;1(7):423-430 PubMed

Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling. Gillham, H., Golding, M.C., Pepperkok, R. & Gullick, W.J. J Cell Biol 1999 Aug 23;146(4):869-80 PubMed

Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes. Shima, D.T., Scales, S.J., Kreis, T.E. & Pepperkok, R. Curr Biol 1999 Jul 29-Aug 12;9(15):821-4 PubMed

Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy. Pepperkok, R., Squire, A., Geley, S. & Bastiaens, P.I. Curr Biol 1999 Mar 11;9(5):269-72 PubMed

The KDEL retrieval system is exploited by Pseudomonas exotoxin A, but not by Shiga-like toxin-1, during retrograde transport from the Golgi complex to the endoplasmic reticulum. Jackson, M.E., Simpson, J.C., Girod, A., Pepperkok, R., Roberts, L.M. & Lord, J.M. J Cell Sci 1999 Feb;112 ( Pt 4):467-75 PubMed

Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S. Pepperkok, R., Lowe, M., Burke, B. & Kreis, T.E. J Cell Sci 1998 Jul;111 ( Pt 13):1877-88 PubMed

Computer-automated and semiautomated capillary microinjection of macromolecules into living cells Pepperkok, R., Saffrich, R. & Ansorge, W. In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press

Cellular micro-biochemistry. Pepperkok, R., Rosorius, O., & Scheel, J. In "Springer Lab Manual: Microinjection and transgenesis of cultured cells and embryos", Cid-Arregui, C. & Garcia-Carranca, A. (eds). Springer, Heidelberg, pp. 145-155

Electroporation of cells Herr, S., Pepperkok, R., Saffrich, R., Wieman, S. & Ansorge, W. In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press

Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI. Scales, S.J., Pepperkok, R. & Kreis, T.E. Cell 1997 Sep 19;90(6):1137-48 PubMed

Dissociation of coatomer from membranes is required for brefeldin A- induced transfer of Golgi enzymes to the endoplasmic reticulum. Scheel, J., Pepperkok, R., Lowe, M., Griffiths, G. & Kreis, T.E. J Cell Biol 1997 Apr 21;137(2):319-33 PubMed

Microinjection and electroporation of macromolecules into live cells. Pepperkok, R.
In "Springer Lab Manual Cell Cycle-Materials and Methods", M. Pagano (ed.) Springer, Heidelberg, pp. 75-87

A fully automated image acquisition and analysis system for low light level fluorescence microscopy. Herr, S., Bastian, T., Pepperkok, R., Boulin, C. & Ansorge, W. Meth Mol Cell Biol 1994 4 164-170

Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Pepperkok, R., Scheel, J., Horstmann, H., Hauri, H.P., Griffiths, G. & Kreis, T.E. Cell 1993 Jul 16;74(1):71-82 PubMed