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Staff at EMBL

Dr. Rainer Pepperkok

Head of Core Facilities, Head of Advanced Light Microscopy Core Facility, Team Leader and Senior Scientist
Core Facilities Pepperkok Team Advanced Light Microscopy Facility

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

E-mail: send a mail


PhD 1992, University Kaiserslautern.
Postdoctoral research at University of Geneva.
Lab head at the Imperial Cancer Research Fund, London.
At EMBL since 1998.
Senior scientist since 2012.
Head of Core Facilities and Scientific Services since 2014.


Plasma membrane translocation of REDD1 governed by GPCRs contributes to mTORC1 activation. Michel, G., Matthes, H.W., Hachet-Haas, M., El Baghdadi, K., de Mey, J., Pepperkok, R., Simpson, J.C., Galzi, J.L. & Lecat, S. J Cell Sci. 2014 Feb 15;127(Pt 4):773-87. doi: 10.1242/jcs.136432. Epub 2013 Dec11. Europe PMC

PIP(3) induces the recycling of receptor tyrosine kinases. Laketa, V., Zarbakhsh, S., Traynor-Kaplan, A., Macnamara, A., Subramanian, D., Putyrski, M., Mueller, R., Nadler, A., Mentel, M., Saez-Rodriguez, J., Pepperkok, R. & Schultz, C. Sci Signal. 2014 Jan 14;7(308):ra5. doi: 10.1126/scisignal.2004532. Europe PMC

An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin. Eskova, A., Knapp, B., Matelska, D., Reusing, S., Arjonen, A., Lisauskas, T., Pepperkok, R., Russell, R., Eils, R., Ivaska, J., Kaderali, L., Erfle, H. & Starkuviene, V. J Cell Sci. 2014 Jun 1;127(Pt 11):2433-47. doi: 10.1242/jcs.137281. Epub 2014 Mar21. Europe PMC

CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum. Sauvageau, E., Rochdi, M.D., Oueslati, M., Hamdan, F.F., Percherancier, Y., Simpson, J.C., Pepperkok, R. & Bouvier, M. Traffic. 2014 Apr;15(4):383-400. doi: 10.1111/tra.12148. Epub 2014 Feb 6. Europe PMC

Adaptive fluorescence microscopy by online feedback image analysis. Tischer, C., Hilsenstein, V., Hanson, K. & Pepperkok, R. Methods Cell Biol. 2014;123C:489-503. doi: 10.1016/B978-0-12-420138-5.00026-4. Europe PMC

Imaging ER-to-Golgi transport: towards a systems view. Verissimo, F. & Pepperkok, R. J Cell Sci. 2013 Nov 15;126(Pt 22):5091-100. doi: 10.1242/jcs.121061. Europe PMC

PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin 1 complex. He, S., Ni, D., Ma, B., Lee, J.H., Zhang, T., Ghozalli, I., Pirooz, S.D., Zhao, Z., Bharatham, N., Li, B., Oh, S., Lee, W.H., Takahashi, Y., Wang, H.G., Minassian, A., Feng, P., Deretic, V., Pepperkok, R., Tagaya, M., Yoon, H.S. & Liang, C. Nat Cell Biol. 2013 Oct;15(10):1206-19. doi: 10.1038/ncb2848. Epub 2013 Sep 22. Europe PMC

High-Content siRNA Screen Reveals Global ENaC Regulators and Potential Cystic Fibrosis Therapy Targets. Almaca, J., Faria, D., Sousa, M., Uliyakina, I., Conrad, C., Sirianant, L., Clarke, L.A., Martins, J.P., Santos, M., Heriche, J.K., Huber, W., Schreiber, R., Pepperkok, R., Kunzelmann, K. & Amaral, M.D. Cell. 2013 Sep 12;154(6):1390-400. doi: 10.1016/j.cell.2013.08.045. Europe PMC

Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding. Iskar, M., Zeller, G., Blattmann, P., Campillos, M., Kuhn, M., Kaminska, K.H., Runz, H., Gavin, A.C., Pepperkok, R., van Noort, V. & Bork, P. Mol Syst Biol. 2013;9:662. doi: 10.1038/msb.2013.20. Europe PMC

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Stadler, C., Rexhepaj, E., Singan, V.R., Murphy, R.F., Pepperkok, R., Uhlen, M., Simpson, J.C. & Lundberg, E. Nat Methods. 2013 Apr;10(4):315-23. doi: 10.1038/nmeth.2377. Epub 2013 Feb 24. Europe PMC

RNAi-Based Functional Profiling of Loci from Blood Lipid Genome-Wide Association Studies Identifies Genes with Cholesterol-Regulatory Function. Blattmann, P., Schuberth, C., Pepperkok, R. & Runz, H. PLoS Genet. 2013 Feb;9(2):e1003338. doi: 10.1371/journal.pgen.1003338. Epub 2013Feb 28. Europe PMC

Cell-to-cell communication: current views and future perspectives. Gerdes, H.H. & Pepperkok, R. Cell Tissue Res. 2013 Apr;352(1):1-3. doi: 10.1007/s00441-013-1590-1. Epub 2013Feb 26. Europe PMC

Golgi depletion from living cells with laser nanosurgery. Ronchi, P. & Pepperkok, R. Methods Cell Biol. 2013;118:311-24. doi: 10.1016/B978-0-12-417164-0.00019-7. Europe PMC

Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia A. Roth, S.D., Schuttrumpf, J., Milanov, P., Abriss, D., Ungerer, C., Quade-Lyssy, P., Simpson, J.C., Pepperkok, R., Seifried, E. & Tonn, T. PLoS One. 2012;7(9):e44505. doi: 10.1371/journal.pone.0044505. Epub 2012 Sep 4. Europe PMC

Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway. Simpson, J.C., Joggerst, B., Laketa, V., Verissimo, F., Cetin, C., Erfle, H., Bexiga, M.G., Singan, V.R., Heriche, J.K., Neumann, B., Mateos, A., Blake, J., Bechtel, S., Benes, V., Wiemann, S., Ellenberg, J. & Pepperkok, R. Nat Cell Biol. 2012 Jun 3;14(7):764-74. doi: 10.1038/ncb2510. Europe PMC

Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1. Moudry, P., Lukas, C., Macurek, L., Neumann, B., Heriche, J.K., Pepperkok, R., Ellenberg, J., Hodny, Z., Lukas, J. & Bartek, J. Cell Death Differ. 2012 May;19(5):798-807. doi: 10.1038/cdd.2011.150. Epub 2011Nov 11. Europe PMC

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. Stadler, C., Hjelmare, M., Neumann, B., Jonasson, K., Pepperkok, R., Uhlen, M. & Lundberg, E. J Proteomics. 2012 Apr 3;75(7):2236-51. doi: 10.1016/j.jprot.2012.01.030. Epub2012 Feb 15. Europe PMC

A new approach to manipulate the fate of single neural stem cells in tissue. Taverna, E., Haffner, C., Pepperkok, R. & Huttner, W.B. Nat Neurosci. 2012;15(2):329-37. doi: 10.1038/nn.3008. Europe PMC

Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements. Seitz, A., Terjung, S., Zimmermann, T. & Pepperkok, R. J Biomed Opt. 2012 Jan;17(1):011010. doi: 10.1117/1.JBO.17.1.011010. Europe PMC

Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Contreras, F.X., Ernst, A.M., Haberkant, P., Bjorkholm, P., Lindahl, E., Gonen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F. & Brugger, B. Nature. 2012 Jan 9;481(7382):525-9. doi: 10.1038/nature10742. Europe PMC

At the cutting edge: applications and perspectives of laser nanosurgery in cell biology. Ronchi, P., Terjung, S. & Pepperkok, R. Biol Chem. 2012 Apr;393(4):235-48. doi: 10.1515/hsz-2011-0237. Europe PMC

Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Ritzerfeld, J., Remmele, S., Wang, T., Temmerman, K., Brugger, B., Wegehingel, S., Tournaviti, S., Strating, J.R., Wieland, F.T., Neumann, B., Ellenberg, J., Lawerenz, C., Hesser, J., Erfle, H., Pepperkok, R. & Nickel, W. Genome Res. 2011 Nov;21(11):1955-68. doi: 10.1101/gr.116087.110. Epub 2011 Jul27. Europe PMC

Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation. Moore, H.M., Bai, B., Boisvert, F.M., Latonen, L., Rantanen, V., Simpson, J.C., Pepperkok, R., Lamond, A.I. & Laiho, M. Mol Cell Proteomics. 2011 Oct;10(10):M111.009241. Epub 2011 Jul 21. Europe PMC

A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells. Tangemo, C., Ronchi, P., Colombelli, J., Haselmann, U., Simpson, J.C., Antony, C., Stelzer, E.H., Pepperkok, R. & Reynaud, E.G. J Cell Sci. 2011 Mar 15;124(Pt 6):978-87. doi: 10.1242/jcs.079640. Europe PMC

Micropilot: automation of fluorescence microscopy-based imaging for systems biology. Conrad, C., Wunsche, A., Tan, T.H., Bulkescher, J., Sieckmann, F., Verissimo, F., Edelstein, A., Walter, T., Liebel, U., Pepperkok, R. & Ellenberg, J. Nat Methods. 2011 Mar;8(3):246-9. doi: 10.1038/nmeth.1558. Epub 2011 Jan 23. Europe PMC

Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. Reiss, S., Rebhan, I., Backes, P., Romero-Brey, I., Erfle, H., Matula, P., Kaderali, L., Poenisch, M., Blankenburg, H., Hiet, M.S., Longerich, T., Diehl, S., Ramirez, F., Balla, T., Rohr, K., Kaul, A., Buhler, S., Pepperkok, R., Lengauer, T., Albrecht, M., Eils, R., Schirmacher, P., Lohmann, V. & Bartenschlager, R. Cell Host Microbe. 2011 Jan 20;9(1):32-45. doi: 10.1016/j.chom.2010.12.002. Europe PMC

Functional genomics assays to study CFTR traffic and ENaC function. Almaca, J., Dahimene, S., Appel, N., Conrad, C., Kunzelmann, K., Pepperkok, R. & Amaral, M.D. Methods Mol Biol. 2011;742:249-64. doi: 10.1007/978-1-61779-120-8_15. Europe PMC

High-throughput microscopy using live mammalian cells. Terjung, S., Walter, T., Seitz, A., Neumann, B., Pepperkok, R. & Ellenberg, J. Cold Spring Harb Protoc. 2010 Aug 1;2010(7):pdb.top84. doi: 10.1101/pdb.top84. Europe PMC

In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays. Grecco, H.E., Roda-Navarro, P., Girod, A., Hou, J., Frahm, T., Truxius, D.C., Pepperkok, R., Squire, A. & Bastiaens, P.I. Nat Methods. 2010 Jun;7(6):467-72. doi: 10.1038/nmeth.1458. Epub 2010 May 9. Europe PMC

Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion. Ebert, A.D., Laussmann, M., Wegehingel, S., Kaderali, L., Erfle, H., Reichert, J., Lechner, J., Beer, H.D., Pepperkok, R. & Nickel, W. Traffic. 2010 Jun;11(6):813-26. doi: 10.1111/j.1600-0854.2010.01059.x. Epub 2010Mar 10. Europe PMC

Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells. Subramanian, D.*, Laketa, V.*, Muller, R., Tischer, C., Zarbakhsh, S., Pepperkok, R. & Schultz, C. * - equally contributing first authors. Nat Chem Biol. 2010 May;6(5):324-6. Epub 2010 Apr 4. Europe PMC

Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging. Walter, T., Held, M., Neumann, B., Heriche, J.K., Conrad, C., Pepperkok, R. & Ellenberg, J. J Struct Biol. 2010 Apr;170(1):1-9. doi: 10.1016/j.jsb.2009.10.004. Epub 2009 Oct23. Europe PMC

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J. Nature. 2010 Apr 1;464(7289):721-7. Europe PMC

From experimental setup to bioinformatics: An RNAi screening platform to identify host factors involved in HIV-1 replication. Borner, K., Hermle, J., Sommer, C., Brown, N.P., Knapp, B., Glass, B., Kunkel, J., Torralba, G., Reymann, J., Beil, N., Beneke, J., Pepperkok, R., Schneider, R., Ludwig, T., Hausmann, M., Hamprecht, F., Erfle, H., Kaderali, L., Krausslich, H.G. & Lehmann, M.J. Biotechnol J. 2009 Dec 10;5(1):39-49. Europe PMC

Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth. Laketa, V., Zarbakhsh, S., Morbier, E., Subramanian, D., Dinkel, C., Brumbaugh, J., Zimmermann, P., Pepperkok, R. & Schultz, C. Chem Biol. 2009 Nov 25;16(11):1190-6. Europe PMC

Factor VIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of factor VIII. Heinz, S., Schuttrumpf, J., Simpson, J.C., Pepperkok, R., Nicolaes, G.A., Abriss, D., Milanov, P., Roth, S., Seifried, E. & Tonn, T. Thromb Haemost. 2009 Nov;102(5):925-35. Europe PMC

DetecTiff: a novel image analysis routine for high-content screening microscopy. Gilbert, D.F., Meinhof, T., Pepperkok, R. & Runz, H. J Biomol Screen. 2009 Sep;14(8):944-55. doi: 10.1177/1087057109339523. Epub 2009Jul 29. Europe PMC

Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins. Tournaviti, S., Pietro, E.S., Terjung, S., Schafmeier, T., Wegehingel, S., Ritzerfeld, J., Schulz, J., Smith, D.F., Pepperkok, R. & Nickel, W. Traffic. 2009 Aug;10(8):1047-60. Epub 2009 May 12. Europe PMC

Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II. Schwarz, K., Iolascon, A., Verissimo, F., Trede, N.S., Horsley, W., Chen, W., Paw, B.H., Hopfner, K.P., Holzmann, K., Russo, R., Esposito, M.R., Spano, D., De Falco, L., Heinrich, K., Joggerst, B., Rojewski, M.T., Perrotta, S., Denecke, J., Pannicke, U., Delaunay, J., Pepperkok, R. & Heimpel, H. Nat Genet. 2009 Aug;41(8):936-40. Epub 2009 Jun 28. Europe PMC

ojoplano-mediated basal constriction is essential for optic cup morphogenesis. Martinez-Morales, J.R., Rembold, M., Greger, K., Simpson, J.C., Brown, K.E., Quiring, R., Pepperkok, R., Martin-Bermudo, M.D., Himmelbauer, H. & Wittbrodt, J. Development. 2009 Jul;136(13):2165-75. Europe PMC

Identification of cholesterol-regulating genes by targeted RNAi screening. Bartz, F., Kern, L., Erz, D., Zhu, M., Gilbert, D., Meinhof, T., Wirkner, U., Erfle, H., Muckenthaler, M., Pepperkok, R. & Runz, H. Cell Metab. 2009 Jul;10(1):63-75. Europe PMC

RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D.H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J. & Lukas, C. Cell. 2009 Feb 6;136(3):435-46. Europe PMC

Rab18 and Rab43 have key roles in ER-Golgi trafficking. Dejgaard, S.Y., Murshid, A., Erman, A., Kizilay, O., Verbich, D., Lodge, R., Dejgaard, K., Ly-Hartig, T.B., Pepperkok, R., Simpson, J.C. & Presley, J.F. J Cell Sci. 2008 Aug 15;121(Pt 16):2768-81. doi: 10.1242/jcs.021808. Epub 2008Jul 29. Europe PMC

Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. Erfle, H., Neumann, B., Rogers, P., Bulkescher, J., Ellenberg, J. & Pepperkok, R. J Biomol Screen. 2008 Aug;13(7):575-80. Epub 2008 Jul 3. Europe PMC

In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum. Pouthas, F., Girard, P., Lecaudey, V., Ly, T.B., Gilmour, D., Boulin, C., Pepperkok, R. & Reynaud, E.G. J Cell Sci. 2008 Jul 15;121(Pt 14):2406-14. Epub 2008 Jun 24. Europe PMC

Human Proteinpedia enables sharing of human protein data. Mathivanan, S., Ahmed, M., Ahn, N.G., Alexandre, H., Amanchy, R., Andrews, P.C., Bader, J.S., Balgley, B.M., Bantscheff, M., Bennett, K.L., Bjorling, E., Blagoev, B., Bose, R., Brahmachari, S.K., Burlingame, A.S., Bustelo, X.R., Cagney, G., Cantin, G.T., Cardasis, H.L., Celis, J.E., Chaerkady, R., Chu, F., Cole, P.A., Costello, C.E., Cotter, R.J., Crockett, D., DeLany, J.P., De Marzo, A.M., DeSouza, L.V., Deutsch, E.W., Dransfield, E., Drewes, G., Droit, A., Dunn, M.J., Elenitoba-Johnson, K., Ewing, R.M., Van Eyk, J., Faca, V., Falkner, J., Fang, X., Fenselau, C., Figeys, D., Gagne, P., Gelfi, C., Gevaert, K., Gimble, J.M., Gnad, F., Goel, R., Gromov, P., Hanash, S.M., Hancock, W.S., Harsha, H.C., Hart, G., Hays, F., He, F., Hebbar, P., Helsens, K., Hermeking, H., Hide, W., Hjerno, K., Hochstrasser, D.F., Hofmann, O., Horn, D.M., Hruban, R.H., Ibarrola, N., James, P., Jensen, O.N., Jensen, P.H., Jung, P., Kandasamy, K., Kheterpal, I., Kikuno, R.F., Korf, U., Korner, R., Kuster, B., Kwon, M.S., Lee, H.J., Lee, Y.J., Lefevre, M., Lehvaslaiho, M., Lescuyer, P., Levander, F., Lim, M.S., Lobke, C., Loo, J.A., Mann, M., Martens, L., Martinez-Heredia, J., McComb, M., McRedmond, J., Mehrle, A., Menon, R., Miller, C.A., Mischak, H., Mohan, S.S., Mohmood, R., Molina, H., Moran, M.F., Morgan, J.D., Moritz, R., Morzel, M., Muddiman, D.C., Nalli, A., Navarro, J.D., Neubert, T.A., Ohara, O., Oliva, R., Omenn, G.S., Oyama, M., Paik, Y.K., Pennington, K., Pepperkok, R., Periaswamy, B., Petricoin, E.F., Poirier, G.G., Prasad, T.S., Purvine, S.O., Rahiman, B.A., Ramachandran, P., Ramachandra, Y.L., Rice, R.H., Rick, J., Ronnholm, R.H., Salonen, J., Sanchez, J.C., Sayd, T., Seshi, B., Shankari, K., Sheng, S.J., Shetty, V., Shivakumar, K., Simpson, R.J., Sirdeshmukh, R., Siu, K.W., Smith, J.C., Smith, R.D., States, D.J., Sugano, S., Sullivan, M., Superti-Furga, G., Takatalo, M., Thongboonkerd, V., Trinidad, J.C., Uhlen, M., Vandekerckhove, J., Vasilescu, J., Veenstra, T.D., Vidal-Taboada, J.M., Vihinen, M., Wait, R., Wang, X., Wiemann, S., Wu, B., Xu, T., Yates, J.R., Zhong, J., Zhou, M., Zhu, Y., Zurbig, P. & Pandey, A. Nat Biotechnol. 2008 Feb;26(2):164-7. doi: 10.1038/nbt0208-164. Europe PMC

ER-to-Golgi transport. Verissimo, F. & Pepperkok, R.. In Mironov, A.A. & Pavelka, M. (eds.) The Golgi Apparatus: State of the art 110 years after Camillo Golgi's discovery (2008), Springer, pp. 333-341.

Integrating systems biology with clinical research. Pepperkok, R. & Wiemann, S. Genome Biol. 2008;9(7):314. Epub 2008 Jul 1. Europe PMC

A correlative light and electron microscopy method based on laser micropatterning and etching. Colombelli, J., Tangemo, C., Haselman, U., Antony, C., Stelzer, E.H., Pepperkok, R. & Reynaud, E.G. Methods Mol Biol. 2008;457:203-13. Europe PMC

The potential of high-content high-throughput microscopy in drug discovery. Starkuviene, V. & Pepperkok, R. Br J Pharmacol. 2007 Sep;152(1):62-71. Epub 2007 Jul 2. Europe PMC

Differential requirements for ts-O45-G and procollagen biosynthetic transport. Starkuviene, V. & Pepperkok, R. Traffic. 2007 Aug;8(8):1035-51. Epub 2007 Jun 5. Europe PMC

Transfected cell microarrays: an efficient tool for high-throughput functional analysis. Starkuviene, V., Pepperkok, R. & Erfle, H. Expert Rev Proteomics. 2007 Aug;4(4):479-89. Europe PMC

An RNAi screening platform to identify secretion machinery in mammalian cells. Simpson, J.C., Cetin, C., Erfle, H., Joggerst, B., Liebel, U., Ellenberg, J. & Pepperkok, R. J Biotechnol. 2007 Apr 30;129(2):352-65. Epub 2007 Jan 14. Europe PMC

Maturation of the mammalian secretome. Simpson, J.C., Mateos, A. & Pepperkok, R. Genome Biol. 2007 Apr 30;8(4):211. Europe PMC

High-content microscopy identifies new neurite outgrowth regulators. Laketa, V., Simpson, J.C., Bechtel, S., Wiemann, S. & Pepperkok, R. Mol Biol Cell. 2007 Jan;18(1):242-52. Epub 2006 Nov 8. Europe PMC

Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. Grabher, C., Cliffe, A., Miura, K., Pepperkok, R., Rorth, P. & Wittbrodt, J. J Leukoc Biol. 2007 Jan;81(1):263-71. Europe PMC

Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome. von Brunn, A., Teepe, C., Simpson, J.C., Pepperkok, R., Friedel, C.C., Zimmer, R., Roberts, R., Baric, R. & Haas, J. PLoS ONE. 2007 May 23;2(5):e459. Europe PMC

Membrane dynamics in the early secretory pathway. Robinson, D.G., Herranz, M.C., Bubeck, J., Pepperkok, R. & Ritzenthaler, C. Critical Reviews in Plant Sciences 2007 26(4) 199-225

Reverse transfection on cell arrays for high content screening microscopy. Erfle, H., Neumann, B., Liebel, U., Rogers, P., Held, M., Walter, T., Ellenberg, J. & Pepperkok, R. Nat Protoc. 2007;2(2):392-9. Europe PMC

A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex. Stauber, T., Simpson, J.C., Pepperkok, R. & Vernos, I. Curr Biol. 2006 Nov 21;16(22):2245-51. Europe PMC

High-throughput fluorescence microscopy for systems biology. Pepperkok, R. & Ellenberg, J. Nat Rev Mol Cell Biol. 2006 Sep;7(9):690-6. Epub 2006 Jul 19. Europe PMC

Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion. Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. & Pepperkok, R. Traffic. 2006 Sep;7(9):1283-9. Europe PMC

Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway. Verissimo, F., Silva, E., Morris, J.D., Pepperkok, R. & Jordan, P. Oncogene. 2006 Jul 13;25(30):4172-82. Epub 2006 Feb 27. Europe PMC

Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G. Runz, H., Miura, K., Weiss, M. & Pepperkok, R. EMBO J. 2006 Jul 12;25(13):2953-65. Epub 2006 Jun 22. Europe PMC

Laser nanosurgery in cell biology Colombelli, J., Pepperkok, R., Stelzer, E.H.K. & Reynaud, E.G. Med Sci (Paris). 2006 Jul-Aug;22(6-7):651-8. Europe PMC

High-throughput RNAi screening by time-lapse imaging of live human cells. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J. Nat Methods. 2006 May;3(5):385-90. Europe PMC

Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells. Tabary, O., Boncoeur, E., de Martin, R., Pepperkok, R., Clement, A., Schultz, C. & Jacquot, J. Cell Signal. 2006 May;18(5):652-60. Epub 2005 Aug 9. Europe PMC

Biogenesis of tubular ER-to-Golgi transport intermediates. Simpson, J.C., Nilsson, T. & Pepperkok, R. Mol Biol Cell. 2006 Feb;17(2):723-37. Epub 2005 Nov 28. Europe PMC

Secretory cargo regulates the turnover of COPII subunits at single ER exit sites. Forster, R., Weiss, M., Zimmermann, T., Reynaud, E.G., Verissimo, F., Stephens, D.J. & Pepperkok, R. Curr Biol. 2006 Jan 24;16(2):173-9. Europe PMC

The subcellular localization of the mammalian proteome comes a fraction closer. Simpson, J.C. & Pepperkok, R. Genome Biol. 2006;7(6):222. Europe PMC

Systematic Subcellular Localization of Novel Proteins. Simpson, J.C. & Pepperkok, R. In Cell Biology. A Laboratory Handbook (3rd ed.), 2006, vol. 3, pp. 121–127.

The LIFEdb database in 2006. Mehrle, A., Rosenfelder, H., Schupp, I., del Val, C., Arlt, D., Hahne, F., Bechtel, S., Simpson, J., Hofmann, O., Hide, W., Glatting, K.H., Huber, W., Pepperkok, R., Poustka, A. & Wiemann, S. Nucleic Acids Res. 2006 Jan 1;34(Database issue):D415-8. Europe PMC

Production of siRNA- and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells. Erfle, H. & Pepperkok, R. Methods Mol Biol. 2006;360:155-62. Europe PMC

In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Colombelli, J., Reynaud, E.G., Rietdorf, J., Pepperkok, R. & Stelzer, E.H.K. Traffic. 2005 Dec;6(12):1093-102. Europe PMC

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization. Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B., Knop, M., Scheffzek, K. & Pepperkok, R. BMC Biol. 2005 Oct 7;3:21. Europe PMC

IR micro spectroscopy of live cells. Moss, D.A., Keese, M., Pepperkok, R. Vibrational Spectroscopy 2005 JUL;38(1-2): 185-191.

Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues. Keese, M., Magdeburg, R.J., Herzog, T., Hasenberg, T., Offterdinger, M., Pepperkok, R., Sturm, J.W. & Bastiaens, P.I. J Biol Chem 2005 Jul 29;280(30):27826-31. Epub 2005 May 20. Europe PMC

Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions. Knauer, S.K., Moodt, S., Berg, T., Liebel, U., Pepperkok, R. & Stauber, R.H. Traffic 2005 Jul;6(7):594-606. Europe PMC

Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Yang, Y.D., Elamawi, R., Bubeck, J., Pepperkok, R., Ritzenthaler, C. & Robinson, D.G. Plant Cell 2005 May;17(5):1513-31. Epub 2005 Apr 1. Europe PMC

Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking. Neubrand, V.E., Will, R.D., Mobius, W., Poustka, A., Wiemann, S., Schu, P., Dotti, C.G., Pepperkok, R. & Simpson, J.C. EMBO J 2005 Mar 23;24(6):1122-33. Epub 2005 Mar 10. Europe PMC

Live imaging of bidirectional traffic from the ERGIC. Ben-Tekaya, H., Miura, K., Pepperkok, R. & Hauri, H.P. J Cell Sci 2005 Jan 15;118(Pt 2):357-67. Epub 2005 Jan 04. Europe PMC

Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'. Young, J., Stauber, T., del Nery, E., Vernos, I., Pepperkok, R. & Nilsson, T. Mol Biol Cell 2005 Jan;16(1):162-77. Epub 2004 Oct 13. Europe PMC

Coupling of ER exit to microtubules through direct interaction of COPII with dynactin. Watson, P., Forster, R., Palmer, K.J., Pepperkok, R. & Stephens, D.J. Nat Cell Biol 2005 Jan;7(1):48-55. Epub 2004 Dec 5. Europe PMC

EAMNET - a resource and teaching framework for advanced light microscopy. Terjung, S., Pepperkok, R., Ankerhold, R., Caiolfa, V.R., Castel, S., Majer, P., Geiger, B., Luini, A., Salamero, J., Shorte, S. & Small, J.V. European Journal of Cell Biology 2005 84 108

Imaging platforms for measurement of membrane trafficking. Pepperkok, R., Simpson, J.C., Rietdorf, J., Cetin, C., Liebel, U., Terjung, S. & Zimmermann, T. Methods Enzymol. 2005;404:8-18. Europe PMC

Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources. Liebel, U., Kindler, B. & Pepperkok, R. Methods Enzymol. 2005;404:19-26. Europe PMC

Arrays of transfected mammalian cells for high content screening microscopy. Erfle, H. & Pepperkok, R. Methods Enzymol. 2005;404:1-8. Europe PMC

Novel cancer relevant cell cycle modulators identified in automated cell-based assays Dorit Arlt, Huber, W., Mamatha Sauermann, Meher Majety, Florian Hahne, Pepperkok, R., Annemarie Poustka, and Stefan Wiemann European Journal of Cell Biology, 84(Suppl. 55):30, 2005

Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle. Arlt, D., Huber, W., Liebel, U., Schmidt, C., Majety, M., Sauermann, M., Rosenfelder, H., Bechtel, S., Mehrle, A., Bannasch, D., Schupp, I., Seiler, M., Simpson, J.C., Hahne, F., Moosmayer, P., Ruschhaupt, M., Guilleaume, B., Wellenreuther, R., Pepperkok, R., Sultmann, H., Poustka, A. & Wiemann, S. Cancer Res 2005 Sep 1;65(17):7733-42. Europe PMC

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts. Brunet, S., Sardon, T., Zimmerman, T., Wittmann, T., Pepperkok, R., Karsenti, E. & Vernos, I. Mol Biol Cell 2004 Dec;15(12):5318-28. Epub . Europe PMC

From ORFeome to biology: a functional genomics pipeline. Wiemann, S., Arlt, D., Huber, W., Wellenreuther, R., Schleeger, S., Mehrle, A., Bechtel, S., Sauermann, M., Korf, U., Pepperkok, R., Sultmann, H. & Poustka, A. Genome Res. 2004 Oct;14(10B):2136-44. Europe PMC

High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Starkuviene, V., Liebel, U., Simpson, J.C., Erfle, H., Poustka, A., Wiemann, S. & Pepperkok, R. Genome Res 2004 Oct;14(10A):1948-56. Europe PMC

siRNA cell arrays for high-content screening microscopy. Erfle, H., Simpson, J.C., Bastiaens, P.I. & Pepperkok, R. Biotechniques 2004 Sep;37(3):454-8, 460, 462. Europe PMC

'Harvester': a fast meta search engine of human protein resources. Liebel, U., Kindler, B. & Pepperkok, R. Bioinformatics 2004 Aug 12;20(12):1962-3. Epub 2004 Feb 26. Europe PMC

Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum. Stephens, D.J. & Pepperkok, R. J Cell Sci 2004 Jul 15;117(Pt 16):3635-44. Europe PMC

Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells. Becker, S., Simpson, J.C., Pepperkok, R., Heinz, S., Herder, C., Grez, M., Seifried, E. & Tonn, T. Thromb Haemost 2004 Jul;92(1):23-35. Europe PMC

Automatic identification of subcellular phenotypes on human cell arrays. Conrad, C., Erfle, H., Warnat, P., Daigle, N., Lorch, T., Ellenberg, J., Pepperkok, R. & Eils, R. Genome Res. 2004 Jun;14(6):1130-6. Europe PMC

LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system. Bannasch, D., Mehrle, A., Glatting, K.H., Pepperkok, R., Poustka, A. & Wiemann, S. Nucleic Acids Res 2004 Jan 1;32 Database issue:D505-8. Europe PMC

A microscope-based screening platform for large-scale functional protein analysis in intact cells. Liebel, U., Starkuviene, V., Erfle, H., Simpson, J.C., Poustka, A., Wiemann, S. & Pepperkok, R. FEBS Lett 2003 Nov 20;554(3):394-8. Europe PMC

CDNAs for functional genomics and proteomics: the German Consortium. Wiemann, S., Mehrle, A., Bechtel, S., Wellenreuther, R., Pepperkok, R. & Poustka, A. C R Biol. 2003 Oct-Nov;326(10-11):1003-9. Europe PMC

Spectral imaging and its applications in live cell microscopy. Zimmermann, T., Rietdorf, J. & Pepperkok, R. FEBS Lett 2003 Jul 3;546(1):87-92. Europe PMC

The German cDNA network: cDNAs, functional genomics and proteomics. Wiemann, S., Bechtel, S., Bannasch, D., Pepperkok, R. & Poustka, A. J Struct Funct Genomics 2003;4(2-3):87-96. Europe PMC

Localizing the proteome. Simpson, J.C. & Pepperkok, R. Genome Biol 2003;4(12):240. Epub 2003 Nov 18. Europe PMC

Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. Zimmermann, T., Rietdorf, J., Girod, A., Georget, V. & Pepperkok, R. FEBS Lett 2002 Nov 6;531(2):245-9. Europe PMC

Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Gruss, O.J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., Karsenti, E., Mattaj, I.W. & Vernos, I. Nat Cell Biol 2002 Nov;4(11):871-9. Europe PMC

Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection. Richards, A.A., Stang, E., Pepperkok, R. & Parton, R.G. Mol Biol Cell 2002 May;13(5):1750-64. Europe PMC

Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells. Stephens, D.J. & Pepperkok, R. J Cell Sci 2002 Mar 15;115(Pt 6):1149-60. Europe PMC

Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells. Runz, H., Rietdorf, J., Tomic, I., de Bernard, M., Beyreuther, K., Pepperkok, R. & Hartmann, T. J Neurosci 2002 Mar 1;22(5):1679-89. Europe PMC

A fully automated high content screening system for cell based genome analyses. Liebel, U.& Pepperkok, R. Transkript Laborwelt 2002 1 4-8

The Spir actin organizers are involved in vesicle transport processes. Kerkhoff, E., Simpson, J.C., Leberfinger, C.B., Otto, I.M., Doerks, T., Bork, P., Rapp, U.R., Raabe, T. & Pepperkok, R. Curr Biol 2001 Dec 11;11(24):1963-8. Europe PMC

The many ways to cross the plasma membrane. Stephens, D.J. & Pepperkok, R. Proc Natl Acad Sci U S A 2001 Apr 10;98(8):4295-8. Europe PMC

Illuminating the secretory pathway: when do we need vesicles? Stephens, D.J. & Pepperkok, R. J Cell Sci 2001 Mar;114(Pt 6):1053-9. Europe PMC

Illuminating the human genome. Simpson, J.C., Neubrand, V.E., Wiemann, S. & Pepperkok, R. Histochem Cell Biol 2001 Jan;115(1):23-9. Europe PMC

Being in the right location at the right time. Pepperkok, R., Simpson, J.C. & Wiemann, S. Genome Biol 2001;2(9):REVIEWS1024. Europe PMC

Observing proteins in their natural habitat: the living cell. Bastiaens, P.I. & Pepperkok, R. Trends Biochem Sci 2000 Dec 1;25(12):631-637 Europe PMC

Breaking the COPI monopoly on Golgi recycling. Storrie, B., Pepperkok, R. & Nilsson, T. Trends Cell Biol. 2000 Sep;10(9):385-91. Europe PMC

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. Simpson, J.C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S. EMBO rep 2000 Sep;1(3):287-92. Europe PMC

COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. Stephens, D.J., Lin-Marq, N., Pagano, A., Pepperkok, R. & Paccaud, J.P. J Cell Sci 2000 Jun;113 ( Pt 12):2177-85. Europe PMC

Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation. Pepperkok, R., Hotz-Wagenblatt, A., Konig, N., Girod, A., Bossemeyer, D. & Kinzel, V. J Cell Biol 2000 Feb 21;148(4):715-26 Europe PMC

The p24 family member p23 is required for early embryonic development. Denzel, A., Otto, F., Girod, A., Pepperkok, R., Watson, R., Rosewell, I., Bergeron, J.J., Solari, R.C. & Owen, M.J. Curr Biol 2000 Jan 13;10(1):55-8 Europe PMC

COPI vesicles accumulating in the presence of a GTP restricted Arf1 mutant are depleted of anterograde and retrograde cargo. Pepperkok, R., Whitney, J.A., Gomez, M. & Kreis, T.E. J Cell Sci 2000;113(Pt 1):135-144 Europe PMC

Immunofluorescence microscopy. Pepperkok, R., Girod, A., Simpson, J. & Rietdorf, J. In "Monoclonal Antibodies", Shepherd, P. & Dean, C. (eds.), Oxford Univ.Press, pp. 355-370

Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita. Heiss, N.S., Girod, A., Salowsky, R., Wiemann, S., Pepperkok, R. & Poustka, A. Hum Mol Genet 1999 Dec;8(13):2515-24 Europe PMC

Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum. Girod, A., Storrie, B., Simpson, J.C., Johannes, L., Goud, B., Roberts, L.M., Lord, J.M., Nilsson, T. & Pepperkok, R. Nat Cell Biolog 1999 Nov;1(7):423-430 Europe PMC

Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling. Gillham, H., Golding, M.C., Pepperkok, R. & Gullick, W.J. J Cell Biol 1999 Aug 23;146(4):869-80 Europe PMC

Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes. Shima, D.T., Scales, S.J., Kreis, T.E. & Pepperkok, R. Curr Biol 1999 Jul 29-Aug 12;9(15):821-4 Europe PMC

Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy. Pepperkok, R., Squire, A., Geley, S. & Bastiaens, P.I. Curr Biol 1999 Mar 11;9(5):269-72 Europe PMC

The KDEL retrieval system is exploited by Pseudomonas exotoxin A, but not by Shiga-like toxin-1, during retrograde transport from the Golgi complex to the endoplasmic reticulum. Jackson, M.E., Simpson, J.C., Girod, A., Pepperkok, R., Roberts, L.M. & Lord, J.M. J Cell Sci 1999 Feb;112 ( Pt 4):467-75 Europe PMC

Fluorescence microscopy of living vertebrate cells. Pepperkok, R., & Shima, G. In "Protein Localisation by Fluorescence Microscopy", V.J. Allan (ed.), Oxford Univ. Press, pp. 109-132

Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S. Pepperkok, R., Lowe, M., Burke, B. & Kreis, T.E. J Cell Sci 1998 Jul;111 ( Pt 13):1877-88 Europe PMC

Computer-automated and semiautomated capillary microinjection of macromolecules into living cells Pepperkok, R., Saffrich, R. & Ansorge, W. In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press

Cellular micro-biochemistry. Pepperkok, R., Rosorius, O., & Scheel, J. In "Springer Lab Manual: Microinjection and transgenesis of cultured cells and embryos", Cid-Arregui, C. & Garcia-Carranca, A. (eds). Springer, Heidelberg, pp. 145-155

Electroporation of cells Herr, S., Pepperkok, R., Saffrich, R., Wieman, S. & Ansorge, W. In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press

Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI. Scales, S.J., Pepperkok, R. & Kreis, T.E. Cell 1997 Sep 19;90(6):1137-48 Europe PMC

Dissociation of coatomer from membranes is required for brefeldin A- induced transfer of Golgi enzymes to the endoplasmic reticulum. Scheel, J., Pepperkok, R., Lowe, M., Griffiths, G. & Kreis, T.E. J Cell Biol 1997 Apr 21;137(2):319-33 Europe PMC

Microinjection and electroporation of macromolecules into live cells. Pepperkok, R. In "Springer Lab Manual Cell Cycle-Materials and Methods", M. Pagano (ed.) Springer, Heidelberg, pp. 75-87

Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Pepperkok, R., Scheel, J., Horstmann, H., Hauri, H.P., Griffiths, G. & Kreis, T.E. Cell 1993 Jul 16;74(1):71-82 Europe PMC

CLIP-170, a Cytoplasmic Linker Protein Mediating Interaction of Endosomes with Microtubules. Scheel, J., Rickard, J., Pierre, P., Henning, D., Karecla, P.I., Pepperkok, R., Joggerst-Thomalla, B., Sawyer, A. Parton, R.G. & Kreis, T.E. In Nato ASI Series 74, pp. 145-157, "Molecular Mechanisms of Membrane Traffic", Morre, D.J., Howell, K.E. & Bergeron, J.J.M. (eds.) (1993), Springer Verlag, Berlin/Heidelberg.

A fully automated image acquisition and analysis system for low light level fluorescence microscopy. Herr, S., Bastian, T., Pepperkok, R., Boulin, C. & Ansorge, W. Meth Mol Cell Biol 1993 40 164-170

β-COP, a Coat Protein of Nonclathrin-Coated Vesicles of the Golgi Complex, is Involved in Transport of Vesicular Stomatitis Virus Glycoprotein. Duden, R., Storrie, B., Pepperkok, R., Scheel, J., Joggerst-Thomalla, B., Sawyer, A., Horstmann, H., Griffiths, G. & Kreis, T.E. In Nato ASI Series 74, pp. 117-126, "Molecular Mechanisms of Membrane Traffic", Morre, D.J., Howell, K.E. & Bergeron, J.J.M. (eds.) (1993), Springer Verlag, Berlin/Heidelberg.