Pepperkok TeamPublications
Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding.
Iskar, M., Zeller, G., Blattmann, P., Campillos, M., Kuhn, M., Kaminska, K.H., Runz, H., Gavin, A.C., Pepperkok, R., van Noort, V. & Bork, P.
Mol Syst Biol. 2013 Apr 30;9:662. doi: 10.1038/msb.2013.20. PubMed
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler, C., Rexhepaj, E., Singan, V.R., Murphy, R.F., Pepperkok, R., Uhlen, M., Simpson, J.C. & Lundberg, E.
Nat Methods. 2013 Apr;10(4):315-23. doi: 10.1038/nmeth.2377. Epub 2013 Feb 24. PubMed
RNAi-Based Functional Profiling of Loci from Blood Lipid Genome-Wide Association Studies Identifies Genes with Cholesterol-Regulatory Function.
Blattmann, P., Schuberth, C., Pepperkok, R. & Runz, H.
PLoS Genet. 2013 Feb;9(2):e1003338. doi: 10.1371/journal.pgen.1003338. Epub 2013Feb 28. PubMed
Cell-to-cell communication: current views and future perspectives.
Gerdes, H.H. & Pepperkok, R.
Cell Tissue Res. 2013 Apr;352(1):1-3. doi: 10.1007/s00441-013-1590-1. Epub 2013Feb 26. PubMed
Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia A.
Roth, S.D., Schuttrumpf, J., Milanov, P., Abriss, D., Ungerer, C., Quade-Lyssy, P., Simpson, J.C., Pepperkok, R., Seifried, E. & Tonn, T.
PLoS One. 2012;7(9):e44505. doi: 10.1371/journal.pone.0044505. Epub 2012 Sep 4. PubMed
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway.
Simpson, J.C., Joggerst, B., Laketa, V., Verissimo, F., Cetin, C., Erfle, H., Bexiga, M.G., Singan, V.R., Heriche, J.K., Neumann, B., Mateos, A., Blake, J., Bechtel, S., Benes, V., Wiemann, S., Ellenberg, J. & Pepperkok, R.
Nat Cell Biol. 2012 Jun 3;14(7):764-74. doi: 10.1038/ncb2510. PubMed
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
Moudry, P., Lukas, C., Macurek, L., Neumann, B., Heriche, J.K., Pepperkok, R., Ellenberg, J., Hodny, Z., Lukas, J. & Bartek, J.
Cell Death Differ. 2012 May;19(5):798-807. doi: 10.1038/cdd.2011.150. Epub 2011Nov 11. PubMed
A new approach to manipulate the fate of single neural stem cells in tissue.
Taverna, E., Haffner, C., Pepperkok, R. & Huttner, W.B.
Nat Neurosci. 2011 Dec 18;15(2):329-37. doi: 10.1038/nn.3008. PubMed
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler, C., Hjelmare, M., Neumann, B., Jonasson, K., Pepperkok, R., Uhlen, M. & Lundberg, E.
J Proteomics. 2012 Feb 15. PubMed
Molecular recognition of a single sphingolipid species by a protein's transmembrane domain.
Contreras, F.X., Ernst, A.M., Haberkant, P., Bjorkholm, P., Lindahl, E., Gonen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F. & Brugger, B.
Nature. 2012 Jan 9;481(7382):525-9. doi: 10.1038/nature10742. PubMed
At the cutting edge: applications and perspectives of laser nanosurgery in cell biology.
Ronchi, P., Terjung, S. & Pepperkok, R.
Biol Chem. 2012 Apr;393(4):235-48. doi: 10.1515/hsz-2011-0237. PubMed
Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching--based fluorescence resonance energy transfer measurements
Seitz, A., Terjung, S., Zimmermann, T. and Pepperkok, R.
J. Biomed. Opt. 17, 011010 (2012), DOI:10.1117/1.JBO.17.1.011010
Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases.
Ritzerfeld, J., Remmele, S., Wang, T., Temmerman, K., Brugger, B., Wegehingel, S., Tournaviti, S., Strating, J.R., Wieland, F.T., Neumann, B., Ellenberg, J., Lawerenz, C., Hesser, J., Erfle, H., Pepperkok, R. & Nickel, W.
Genome Res. 2011 Nov;21(11):1955-68. doi: 10.1101/gr.116087.110. Epub 2011 Jul27. PubMed
Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation.
Moore, H.M., Bai, B., Boisvert, F.M., Latonen, L., Rantanen, V., Simpson, J.C., Pepperkok, R., Lamond, A.I. & Laiho, M.
Mol Cell Proteomics. 2011 Oct;10(10):M111.009241. Epub 2011 Jul 21. PubMed
A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells.
Tangemo, C., Ronchi, P., Colombelli, J., Haselmann, U., Simpson, J.C., Antony, C., Stelzer, E.H., Pepperkok, R. & Reynaud, E.G.
J Cell Sci. 2011 Mar 15;124(Pt 6):978-87. doi: 10.1242/jcs.079640. PubMed
Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
Conrad, C., Wunsche, A., Tan, T.H., Bulkescher, J., Sieckmann, F., Verissimo, F., Edelstein, A., Walter, T., Liebel, U., Pepperkok, R. & Ellenberg, J.
Nat Methods. 2011 Mar;8(3):246-9. doi: 10.1038/nmeth.1558. Epub 2011 Jan 23. PubMed
Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.
Reiss, S., Rebhan, I., Backes, P., Romero-Brey, I., Erfle, H., Matula, P., Kaderali, L., Poenisch, M., Blankenburg, H., Hiet, M.S., Longerich, T., Diehl, S., Ramirez, F., Balla, T., Rohr, K., Kaul, A., Buhler, S., Pepperkok, R., Lengauer, T., Albrecht, M., Eils, R., Schirmacher, P., Lohmann, V. & Bartenschlager, R.
Cell Host Microbe. 2011 Jan 20;9(1):32-45. doi: 10.1016/j.chom.2010.12.002. PubMed
Functional genomics assays to study CFTR traffic and ENaC function.
Almaca, J., Dahimene, S., Appel, N., Conrad, C., Kunzelmann, K., Pepperkok, R. & Amaral, M.D.
Methods Mol Biol. 2011;742:249-64. doi: 10.1007/978-1-61779-120-8_15. PubMed
In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays.
Grecco, H.E., Roda-Navarro, P., Girod, A., Hou, J., Frahm, T., Truxius, D.C., Pepperkok, R., Squire, A. & Bastiaens, P.I.
Nat Methods. 2010 Jun;7(6):467-72. doi: 10.1038/nmeth.1458. Epub 2010 May 9. PubMed
Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells.
Subramanian, D.*, Laketa, V.*, Muller, R., Tischer, C., Zarbakhsh, S., Pepperkok, R. & Schultz, C. * - equally contributing first authors.
Nat Chem Biol. 2010 May;6(5):324-6. Epub 2010 Apr 4. PubMed
Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging.
Walter, T., Held, M., Neumann, B., Heriche, J.K., Conrad, C., Pepperkok, R. & Ellenberg, J.
J Struct Biol. 2010 Apr;170(1):1-9. doi: 10.1016/j.jsb.2009.10.004. Epub 2009 Oct23. PubMed
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J.
Nature. 2010 Apr 1;464(7289):721-7. PubMed
High-Throughput Microscopy Using Live Mammalian Cells.
Terjung, S., Walter, T., Seitz, A., Neumann, B., Pepperkok, R. & Ellenberg, J.
In 'Live Cell Imaging: A Laboratory Manual', Second Edition, 2010 (eds. R.D. Goldman, J.R. Swedlow & D.L. Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth.
Laketa, V., Zarbakhsh, S., Morbier, E., Subramanian, D., Dinkel, C., Brumbaugh, J., Zimmermann, P., Pepperkok, R. & Schultz, C.
Chem Biol. 2009 Nov 25;16(11):1190-6. PubMed
Factor VIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of factor VIII.
Heinz, S., Schuttrumpf, J., Simpson, J.C., Pepperkok, R., Nicolaes, G.A., Abriss, D., Milanov, P., Roth, S., Seifried, E. & Tonn, T.
Thromb Haemost. 2009 Nov;102(5):925-35. PubMed
Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins.
Tournaviti, S., Pietro, E.S., Terjung, S., Schafmeier, T., Wegehingel, S., Ritzerfeld, J., Schulz, J., Smith, D.F., Pepperkok, R. & Nickel, W.
Traffic. 2009 Aug;10(8):1047-60. Epub 2009 May 12. PubMed
Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II.
Schwarz, K., Iolascon, A., Verissimo, F., Trede, N.S., Horsley, W., Chen, W., Paw, B.H., Hopfner, K.P., Holzmann, K., Russo, R., Esposito, M.R., Spano, D., De Falco, L., Heinrich, K., Joggerst, B., Rojewski, M.T., Perrotta, S., Denecke, J., Pannicke, U., Delaunay, J., Pepperkok, R. & Heimpel, H.
Nat Genet. 2009 Aug;41(8):936-40. Epub 2009 Jun 28. PubMed
DetecTiff(C): A Novel Image Analysis Routine for High-Content Screening Microscopy.
Gilbert, D.F., Meinhof, T., Pepperkok, R. & Runz, H.
J Biomol Screen. 2009 Jul 29. PubMed
ojoplano-mediated basal constriction is essential for optic cup morphogenesis.
Martinez-Morales, J.R., Rembold, M., Greger, K., Simpson, J.C., Brown, K.E., Quiring, R., Pepperkok, R., Martin-Bermudo, M.D., Himmelbauer, H. & Wittbrodt, J.
Development. 2009 Jul;136(13):2165-75. PubMed
Identification of cholesterol-regulating genes by targeted RNAi screening.
Bartz, F., Kern, L., Erz, D., Zhu, M., Gilbert, D., Meinhof, T., Wirkner, U., Erfle, H., Muckenthaler, M., Pepperkok, R. & Runz, H.
Cell Metab. 2009 Jul;10(1):63-75. PubMed
RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins.
Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D.H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J. & Lukas, C.
Cell. 2009 Feb 6;136(3):435-46. PubMed
Rab18 and Rab43 have key roles in ER-Golgi trafficking
Dejgaard, SY; Murshid, A; Erman, A; Kizilay, O; Verbich, D; Lodge, R; Dejgaard, K; Ly-Hartig, TBN; Pepperkok, R.; Simpson, JC; Presley, JF
JOURNAL OF CELL SCIENCE 121 (16): 2768-2781 AUG 15 2008 PubMed
Rab18 and Rab43 have key roles in ER-Golgi trafficking.
Dejgaard, S.Y., Murshid, A., Erman, A., Kizilay, O., Verbich, D., Lodge, R., Dejgaard, K., Ly-Hartig, T.B., Pepperkok, R., Simpson, J.C. & Presley, J.F.
J Cell Sci. 2008 Aug 15;121(Pt 16):2768-81. Epub 2008 Jul 29. PubMed
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates.
Erfle, H., Neumann, B., Rogers, P., Bulkescher, J., Ellenberg, J. & Pepperkok, R.
J Biomol Screen. 2008 Aug;13(7):575-80. Epub 2008 Jul 3. PubMed
In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum.
Pouthas, F., Girard, P., Lecaudey, V., Ly, T.B., Gilmour, D., Boulin, C., Pepperkok, R. & Reynaud, E.G.
J Cell Sci. 2008 Jul 15;121(Pt 14):2406-14. Epub 2008 Jun 24. PubMed
Integrating systems biology with clinical research.
Pepperkok, R. & Wiemann, S.
Genome Biol. 2008;9(7):314. Epub 2008 Jul 1. PubMed
A correlative light and electron microscopy method based on laser micropatterning and etching.
Colombelli J, Tängemo C, Haselmann U, Antony, C., Stelzer, E.H.K., Pepperkok, R., Reynaud EG
Methods in Molecular Biology, Vol. 457: Membrane Trafficking, A. Vancura (ed), Humana Press, 2008, p.203-214
Transfected cell microarrays: an efficient tool for high-throughput functional analysis.
Starkuviene, V., Pepperkok, R. & Erfle, H.
Expert Rev Proteomics. 2007 Aug;4(4):479-89. PubMed
The potential of high-content high-throughput microscopy in drug discovery.
Starkuviene, V. & Pepperkok, R.
Br J Pharmacol. 2007 Jul 2;. PubMed
Differential Requirements for ts-O45-G and Procollagen Biosynthetic Transport.
Starkuviene, V. & Pepperkok, R.
Traffic. 2007 Jun 5;. PubMed
Maturation of the mammalian secretome.
Simpson, J.C., Mateos, A. & Pepperkok, R.
Genome Biol. 2007 Apr 30;8(4):211. PubMed
An RNAi screening platform to identify secretion machinery in mammalian cells.
Simpson, J.C., Cetin, C., Erfle, H., Joggerst, B., Liebel, U., Ellenberg, J. & Pepperkok, R.
J Biotechnol. 2007 Apr 30;129(2):352-65. Epub 2007 Jan 14. PubMed
High-content microscopy identifies new neurite outgrowth regulators.
Laketa, V., Simpson, J.C., Bechtel, S., Wiemann, S. & Pepperkok, R.
Mol Biol Cell. 2007 Jan;18(1):242-52. Epub 2006 Nov 8. PubMed
Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka.
Grabher, C., Cliffe, A., Miura, K., Pepperkok, R., Rorth, P. & Wittbrodt, J.
J Leukoc Biol. 2007 Jan;81(1):263-71. PubMed
Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome.
von Brunn, A., Teepe, C., Simpson, J.C., Pepperkok, R., Friedel, C.C., Zimmer, R., Roberts, R., Baric, R. & Haas, J.
PLoS ONE. 2007 May 23;2(5):e459. PubMed
Membrane dynamics in the early secretory pathway.
Robinson, DG; Herranz, MC; Bubeck, J; Pepperkok, R.; Ritzenthaler, C
Critical Reviews in Plant Sciences 2007 26(4)
Reverse transfection on cell arrays for high content screening microscopy.
Erfle, H., Neumann, B., Liebel, U., Rogers, P., Held, M., Walter, T., Ellenberg, J. & Pepperkok, R.
Nat Protoc. 2007;2(2):392-9. PubMed
A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex.
Stauber, T., Simpson, J.C., Pepperkok, R. & Vernos, I.
Curr Biol. 2006 Nov 21;16(22):2245-51. PubMed
Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.
Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. & Pepperkok, R.
Traffic. 2006 Sep;7(9):1283-9. PubMed
Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway.
Verissimo, F., Silva, E., Morris, J.D., Pepperkok, R. & Jordan, P.
Oncogene. 2006 Jul 13;25(30):4172-82. Epub 2006 Feb 27. PubMed
Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G.
Runz, H., Miura, K., Weiss, M. & Pepperkok, R.
EMBO J. 2006 Jul 12;25(13):2953-65. Epub 2006 Jun 22. PubMed
Laser nanosurgery in cell biology
Colombelli, J., Pepperkok, R., Stelzer, E.H.K. & Reynaud, E.G.
Med Sci (Paris). 2006 Jul-Aug;22(6-7):651-8. PubMed
Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells.
Tabary, O., Boncoeur, E., de Martin, R., Pepperkok, R., Clement, A., Schultz, C. & Jacquot, J.
Cell Signal. 2006 May;18(5):652-60. Epub 2005 Aug 9. PubMed
High-throughput RNAi screening by time-lapse imaging of live human cells.
Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J.
Nat Methods. 2006 May;3(5):385-90. PubMed
Biogenesis of tubular ER-to-Golgi transport intermediates.
Simpson, J.C., Nilsson, T. & Pepperkok, R.
Mol Biol Cell. 2006 Feb;17(2):723-37. Epub 2005 Nov 28. PubMed
Secretory cargo regulates the turnover of COPII subunits at single ER exit sites.
Forster, R., Weiss, M., Zimmermann, T., Reynaud, E.G., Verissimo, F., Stephens, D.J. & Pepperkok, R.
Curr Biol. 2006 Jan 24;16(2):173-9. PubMed
The subcellular localization of the mammalian proteome comes a fraction closer.
Simpson, J.C. & Pepperkok, R.
Genome Biol. 2006;7(6):222. PubMed
The LIFEdb database in 2006.
Mehrle, A., Rosenfelder, H., Schupp, I., del Val, C., Arlt, D., Hahne, F., Bechtel, S., Simpson, J., Hofmann, O., Hide, W., Glatting, K.H., Huber, W., Pepperkok, R., Poustka, A. & Wiemann, S.
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D415-8. PubMed
Production of siRNA- and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells.
Erfle, H. & Pepperkok, R.
Methods Mol Biol. 2006;360:155-62. PubMed
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery.
Colombelli, J., Reynaud, E.G., Rietdorf, J., Pepperkok, R. & Stelzer, E.H.K.
Traffic. 2005 Dec;6(12):1093-102. PubMed
Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization.
Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B., Knop, M., Scheffzek, K. & Pepperkok, R.
BMC Biol. 2005 Oct 7;3:21. PubMed
IR micro spectroscopy of live cells.
Moss, D.A., Keese, M., Pepperkok, R.
Vibrational Spectroscopy 2005 JUL;38(1-2): 185-191.
Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues.
Keese, M., Magdeburg, R.J., Herzog, T., Hasenberg, T., Offterdinger, M., Pepperkok, R., Sturm, J.W. & Bastiaens, P.I.
J Biol Chem 2005 Jul 29;280(30):27826-31. Epub 2005 May 20. PubMed
Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions.
Knauer, S.K., Moodt, S., Berg, T., Liebel, U., Pepperkok, R. & Stauber, R.H.
Traffic 2005 Jul;6(7):594-606. PubMed
Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites.
Yang, Y.D., Elamawi, R., Bubeck, J., Pepperkok, R., Ritzenthaler, C. & Robinson, D.G.
Plant Cell 2005 May;17(5):1513-31. Epub 2005 Apr 1. PubMed
Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking.
Neubrand, V.E., Will, R.D., Mobius, W., Poustka, A., Wiemann, S., Schu, P., Dotti, C.G., Pepperkok, R. & Simpson, J.C.
EMBO J 2005 Mar 23;24(6):1122-33. Epub 2005 Mar 10. PubMed
Live imaging of bidirectional traffic from the ERGIC.
Ben-Tekaya, H., Miura, K., Pepperkok, R. & Hauri, H.P.
J Cell Sci 2005 Jan 15;118(Pt 2):357-67. Epub 2005 Jan 04. PubMed
Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'.
Young, J., Stauber, T., del Nery, E., Vernos, I., Pepperkok, R. & Nilsson, T.
Mol Biol Cell 2005 Jan;16(1):162-77. Epub 2004 Oct 13. PubMed
Coupling of ER exit to microtubules through direct interaction of COPII with dynactin.
Watson, P., Forster, R., Palmer, K.J., Pepperkok, R. & Stephens, D.J.
Nat Cell Biol 2005 Jan;7(1):48-55. Epub 2004 Dec 5. PubMed
EAMNET - a resource and teaching framework for advanced light microscopy.
Terjung, S., Pepperkok, R., Ankerhold, R., Caiolfa, V.R., Castel, S., Majer, P., Geiger, B., Luini, A., Salamero, J., Shorte, S. & Small, J.V.
European Journal of Cell Biology 2005 84 108
Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources.
Liebel, U., Kindler, B. & Pepperkok, R.
Methods Enzymol. 2005;404:19-26. PubMed
Arrays of transfected mammalian cells for high content screening microscopy.
Erfle, H. & Pepperkok, R.
Methods Enzymol. 2005;404:1-8. PubMed
Novel cancer relevant cell cycle modulators identified in automated cell-based assays
Dorit Arlt, Huber, W., Mamatha Sauermann, Meher Majety, Florian Hahne, Pepperkok, R., Annemarie Poustka, and Stefan Wiemann
European Journal of Cell Biology, 84(Suppl. 55):30, 2005
Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle.
Arlt, D., Huber, W., Liebel, U., Schmidt, C., Majety, M., Sauermann, M., Rosenfelder, H., Bechtel, S., Mehrle, A., Bannasch, D., Schupp, I., Seiler, M., Simpson, J.C., Hahne, F., Moosmayer, P., Ruschhaupt, M., Guilleaume, B., Wellenreuther, R., Pepperkok, R., Sultmann, H., Poustka, A. & Wiemann, S.
Cancer Res 2005 Sep 1;65(17):7733-42. PubMed
Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.
Brunet, S., Sardon, T., Zimmerman, T., Wittmann, T., Pepperkok, R., Karsenti, E. & Vernos, I.
Mol Biol Cell 2004 Dec;15(12):5318-28. Epub . PubMed
From ORFeome to biology: a functional genomics pipeline.
Wiemann, S., Arlt, D., Huber, W., Wellenreuther, R., Schleeger, S., Mehrle, A., Bechtel, S., Sauermann, M., Korf, U., Pepperkok, R., Sultmann, H. & Poustka, A.
Genome Res. 2004 Oct;14(10B):2136-44. PubMed
High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic.
Starkuviene, V., Liebel, U., Simpson, J.C., Erfle, H., Poustka, A., Wiemann, S. & Pepperkok, R.
Genome Res 2004 Oct;14(10A):1948-56. PubMed
siRNA cell arrays for high-content screening microscopy.
Erfle, H., Simpson, J.C., Bastiaens, P.I. & Pepperkok, R.
Biotechniques 2004 Sep;37(3):454-8, 460, 462. PubMed
'Harvester': a fast meta search engine of human protein resources.
Liebel, U., Kindler, B. & Pepperkok, R.
Bioinformatics 2004 Aug 12;20(12):1962-3. Epub 2004 Feb 26. PubMed
Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum.
Stephens, D.J. & Pepperkok, R.
J Cell Sci 2004 Jul 15;117(Pt 16):3635-44. PubMed
Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells.
Becker, S., Simpson, J.C., Pepperkok, R., Heinz, S., Herder, C., Grez, M., Seifried, E. & Tonn, T.
Thromb Haemost 2004 Jul;92(1):23-35. PubMed
Automatic identification of subcellular phenotypes on human cell arrays.
Conrad, C., Erfle, H., Warnat, P., Daigle, N., Lorch, T., Ellenberg, J., Pepperkok, R. & Eils, R.
Genome Res 2004 Jun;14(6):1130-6. PubMed
LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system.
Bannasch, D., Mehrle, A., Glatting, K.H., Pepperkok, R., Poustka, A. & Wiemann, S.
Nucleic Acids Res 2004 Jan 1;32 Database issue:D505-8. PubMed
A microscope-based screening platform for large-scale functional protein analysis in intact cells.
Liebel, U., Starkuviene, V., Erfle, H., Simpson, J.C., Poustka, A., Wiemann, S. & Pepperkok, R.
FEBS Lett 2003 Nov 20;554(3):394-8. PubMed
Spectral imaging and its applications in live cell microscopy.
Zimmermann, T., Rietdorf, J. & Pepperkok, R.
FEBS Lett 2003 Jul 3;546(1):87-92. PubMed
The German cDNA network: cDNAs, functional genomics and proteomics.
Wiemann, S., Bechtel, S., Bannasch, D., Pepperkok, R. & Poustka, A.
J Struct Funct Genomics 2003;4(2-3):87-96. PubMed
Localizing the proteome.
Simpson, J.C. & Pepperkok, R.
Genome Biol 2003;4(12):240. Epub 2003 Nov 18. PubMed
Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair.
Zimmermann, T., Rietdorf, J., Girod, A., Georget, V. & Pepperkok, R.
FEBS Lett 2002 Nov 6;531(2):245-9. PubMed
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.
Gruss, O.J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., Karsenti, E., Mattaj, I.W. & Vernos, I.
Nat Cell Biol 2002 Nov;4(11):871-9. PubMed
Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection.
Richards, A.A., Stang, E., Pepperkok, R. & Parton, R.G.
Mol Biol Cell 2002 May;13(5):1750-64. PubMed
Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells.
Stephens, D.J. & Pepperkok, R.
J Cell Sci 2002 Mar 15;115(Pt 6):1149-60. PubMed
Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells.
Runz, H., Rietdorf, J., Tomic, I., de Bernard, M., Beyreuther, K., Pepperkok, R. & Hartmann, T.
J Neurosci 2002 Mar 1;22(5):1679-89. PubMed
A fully automated high content screening system for cell based genome analyses.
Liebel, U.& Pepperkok, R.
Transkript Laborwelt 2002 1 4-8
The Spir actin organizers are involved in vesicle transport processes.
Kerkhoff, E., Simpson, J.C., Leberfinger, C.B., Otto, I.M., Doerks, T., Bork, P., Rapp, U.R., Raabe, T. & Pepperkok, R.
Curr Biol 2001 Dec 11;11(24):1963-8. PubMed
The many ways to cross the plasma membrane.
Stephens, D.J. & Pepperkok, R.
Proc Natl Acad Sci U S A 2001 Apr 10;98(8):4295-8. PubMed
Illuminating the secretory pathway: when do we need vesicles?
Stephens, D.J. & Pepperkok, R.
J Cell Sci 2001 Mar;114(Pt 6):1053-9. PubMed
Illuminating the human genome.
Simpson, J.C., Neubrand, V.E., Wiemann, S. & Pepperkok, R.
Histochem Cell Biol 2001 Jan;115(1):23-9. PubMed
Being in the right location at the right time.
Pepperkok, R., Simpson, J.C. & Wiemann, S.
Genome Biol 2001;2(9):REVIEWS1024. PubMed
Observing proteins in their natural habitat: the living cell.
Bastiaens, P.I. & Pepperkok, R.
Trends Biochem Sci 2000 Dec 1;25(12):631-637 PubMed
Breaking the COPI monopoly on Golgi recycling.
Storrie, B., Pepperkok, R. & Nilsson, T.
Trends Cell Biol. 2000 Sep;10(9):385-91. PubMed
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Simpson, J.C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S.
EMBO rep 2000 Sep;1(3):287-92. PubMed
COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites.
Stephens, D.J., Lin-Marq, N., Pagano, A., Pepperkok, R. & Paccaud, J.P.
J Cell Sci 2000 Jun;113 ( Pt 12):2177-85. PubMed
Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation.
Pepperkok, R., Hotz-Wagenblatt, A., Konig, N., Girod, A., Bossemeyer, D. & Kinzel, V.
J Cell Biol 2000 Feb 21;148(4):715-26 PubMed
The p24 family member p23 is required for early embryonic development.
Denzel, A., Otto, F., Girod, A., Pepperkok, R., Watson, R., Rosewell, I., Bergeron, J.J., Solari, R.C. & Owen, M.J.
Curr Biol 2000 Jan 13;10(1):55-8 PubMed
COPI vesicles accumulating in the presence of a GTP restricted Arf1 mutant are depleted of anterograde and retrograde cargo.
Pepperkok, R., Whitney, J.A., Gomez, M. & Kreis, T.E.
J Cell Sci 2000;113(Pt 1):135-144 PubMed
Fluorescence microscopy of living vertebrate cells.
Pepperkok, R., & Shima, G.
In "Protein Localisation by Fluorescence Microscopy", V.J. Allan (ed.), Oxford Univ. Press, pp. 109-132
Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita.
Heiss, N.S., Girod, A., Salowsky, R., Wiemann, S., Pepperkok, R. & Poustka, A.
Hum Mol Genet 1999 Dec;8(13):2515-24 PubMed
Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum.
Girod, A., Storrie, B., Simpson, J.C., Johannes, L., Goud, B., Roberts, L.M., Lord, J.M., Nilsson, T. & Pepperkok, R.
Nat Cell Biolog 1999 Nov;1(7):423-430 PubMed
Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling.
Gillham, H., Golding, M.C., Pepperkok, R. & Gullick, W.J.
J Cell Biol 1999 Aug 23;146(4):869-80 PubMed
Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes.
Shima, D.T., Scales, S.J., Kreis, T.E. & Pepperkok, R.
Curr Biol 1999 Jul 29-Aug 12;9(15):821-4 PubMed
Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy.
Pepperkok, R., Squire, A., Geley, S. & Bastiaens, P.I.
Curr Biol 1999 Mar 11;9(5):269-72 PubMed
The KDEL retrieval system is exploited by Pseudomonas exotoxin A, but not by Shiga-like toxin-1, during retrograde transport from the Golgi complex to the endoplasmic reticulum.
Jackson, M.E., Simpson, J.C., Girod, A., Pepperkok, R., Roberts, L.M. & Lord, J.M.
J Cell Sci 1999 Feb;112 ( Pt 4):467-75 PubMed
Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S.
Pepperkok, R., Lowe, M., Burke, B. & Kreis, T.E.
J Cell Sci 1998 Jul;111 ( Pt 13):1877-88 PubMed
Computer-automated and semiautomated capillary microinjection of macromolecules into living cells
Pepperkok, R., Saffrich, R. & Ansorge, W.
In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press
Cellular micro-biochemistry.
Pepperkok, R., Rosorius, O., & Scheel, J.
In "Springer Lab Manual: Microinjection and transgenesis of cultured cells and embryos", Cid-Arregui, C. & Garcia-Carranca, A. (eds). Springer, Heidelberg, pp. 145-155
Electroporation of cells
Herr, S., Pepperkok, R., Saffrich, R., Wieman, S. & Ansorge, W.
In "Cell Biology: A Laboratory Handbook, 2nd Edition", Vol 4, Celis, J.E. (ed.), 1998, Academic Press
Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI.
Scales, S.J., Pepperkok, R. & Kreis, T.E.
Cell 1997 Sep 19;90(6):1137-48 PubMed
Dissociation of coatomer from membranes is required for brefeldin A- induced transfer of Golgi enzymes to the endoplasmic reticulum.
Scheel, J., Pepperkok, R., Lowe, M., Griffiths, G. & Kreis, T.E.
J Cell Biol 1997 Apr 21;137(2):319-33 PubMed
Microinjection and electroporation of macromolecules into live cells.
Pepperkok, R.
In "Springer Lab Manual Cell Cycle-Materials and Methods", M. Pagano (ed.) Springer, Heidelberg, pp. 75-87
A fully automated image acquisition and analysis system for low light level fluorescence microscopy.
Herr, S., Bastian, T., Pepperkok, R., Boulin, C. & Ansorge, W.
Meth Mol Cell Biol 1994 4 164-170
Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo.
Pepperkok, R., Scheel, J., Horstmann, H., Hauri, H.P., Griffiths, G. & Kreis, T.E.
Cell 1993 Jul 16;74(1):71-82 PubMed
