Staff at EMBL

Dr. Carsten Schultz

Interdisciplinary Group Leader and Senior Scientist
Schultz Group

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Biography

PhD 1989, University of Bremen, Germany.
Postdoctoral research at the University of California, San Diego, USA.
Habilitation 1997, Organic Chemistry, University of Bremen, Germany.
Group Leader, MPI for Molecular Physiology, Dortmund, Germany.
Group Leader at EMBL since 2001.

Publications

In Vivo profiling and visualization of cellular protein-lipid interactions using bifunctional Fatty acids. Haberkant, P., Raijmakers, R., Wildwater, M., Sachsenheimer, T., Brugger, B., Maeda, K., Houweling, M., Gavin, A.C., Schultz, C., van Meer, G., Heck, A.J. & Holthuis, J.C. Angew Chem Int Ed Engl. 2013 Apr 2;52(14):4033-8. doi: 10.1002/anie.201210178.Epub 2013 Feb 28. PubMed

The power of fluorogenic probes. Nadler, A. & Schultz, C. Angew Chem Int Ed Engl. 2013 Feb 25;52(9):2408-10. doi: 10.1002/anie.201209733.Epub 2013 Jan 21. PubMed

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Lukinavicius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Correa IR, J.r, Luo, Z.G., Schultz, C., Lemke, E.A., Heppenstall, P., Eggeling, C., Manley, S. & Johnsson, K. Nat Chem. 2013 Feb;5(2):132-9. doi: 10.1038/nchem.1546. Epub 2013 Jan 6. PubMed

HyPer-3: A Genetically Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging. Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella, T.W., Grabher, C., Schultz, C., Lukyanov, S. & Belousov, V.V. ACS Chem Biol. 2013 Mar 15;8(3):535-42. doi: 10.1021/cb300625g. Epub 2013 Jan 7. PubMed

The fatty acid composition of diacylglycerols determines local signaling patterns. Nadler, A., Reither, G., Feng, S., Stein, F., Reither, S., Mueller, R. & Schultz, C. Angew. Chem. Int. Ed. 52, in press (2013). DOI: 10.1002/anie.201301716

Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation. Borrmann, A., Milles, S., Plass, T., Dommerholt, J., Verkade, J.M., Wiessler, M., Schultz, C., van Hest, J.C., van Delft, F.L. & Lemke, E.A. Chembiochem. 2012 Sep 24;13(14):2094-9. doi: 10.1002/cbic.201200407. Epub 2012Sep 3. PubMed

The chemical biology of phosphoinositide 3-kinases. Wymann, M.P. & Schultz, C. Chembiochem. 2012 Sep 24;13(14):2022-35. doi: 10.1002/cbic.201200089. Epub 2012Sep 10. PubMed

Protein tango: the toolbox to capture interacting partners. Rutkowska, A. & Schultz, C. Angew Chem Int Ed Engl. 2012 Aug 13;51(33):8166-76. doi: 10.1002/anie.201201717.Epub 2012 Jun 11. PubMed

Target-activated prodrugs for the auto-regulated inhibition of MMP12. Cobos-Correa, A., Stein, F., Schultz, C. ACS Med. Chem. Lett., 2012, 3 (8), pp 653-657, doi: 10.1021/ml3001193.

Can We See PIP(3) and Hydrogen Peroxide with a Single Probe? Mishina, N.M., Bogeski, I., Bolotin, D.A., Hoth, M., Niemeyer, B.A., Schultz, C., Zagaynova, E.V., Lukyanov, S. & Belousov, V.V. Antioxid Redox Signal. 2012 Aug 1;17(3):505-12. Epub 2012 Apr 10. PubMed

Protein translocation as a tool: The current rapamycin story. Putyrski, M. & Schultz, C. FEBS Lett. 2012 Jul 16;586(15):2097-105. doi: 10.1016/j.febslet.2012.04.061. Epub2012 May 11. PubMed

Conformational Analysis of a Genetically Encoded FRET Biosensor by SAXS. Mertens, H.D., Piljic, A., Schultz, C. & Svergun, D.I. Biophys J. 2012 Jun 20;102(12):2866-75. Epub 2012 Jun 19. PubMed

Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters. Gehrig, S., Mall, M.A. & Schultz, C. Angew Chem Int Ed Engl. 2012 Jun 18;51(25):6258-61. doi: 10.1002/anie.201109226.Epub 2012 May 3. PubMed

Amino acids for diels-alder reactions in living cells. Plass, T., Milles, S., Koehler, C., Szymanski, J., Mueller, R., Wiessler, M., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2012 Apr 23;51(17):4166-70. doi: 10.1002/anie.201108231.Epub 2012 Mar 30. PubMed

CFTR Regulates Early Pathogenesis of Chronic Obstructive Lung Disease in betaENaC-Overexpressing Mice. Johannesson, B., Hirtz, S., Schatterny, J., Schultz, C. & Mall, M.A. PLoS One. 2012;7(8):e44059. Epub 2012 Aug 24. PubMed

A FlAsH-Based Cross-Linker to Study Protein Interactions in Living Cells. Rutkowska, A., Häring, C.H. & Schultz, C. Angew Chem Int Ed Engl. 2011 Dec 23;50(52):12655-8. doi:10.1002/anie.201106404. Epub 2011 Nov 16. PubMed

Switching heterotrimeric G protein subunits with a chemical dimerizer. Putyrski, M. & Schultz, C. Chem Biol. 2011 Sep 23;18(9):1126-33. PubMed

Principles for designing fluorescent sensors and reporters. Lemke, E.A. & Schultz, C. Nat Chem Biol. 2011 Jul 18;7(8):480-3. doi: 10.1038/nchembio.620. PubMed

Rapid development of genetically encoded FRET reporters. Piljic, A., de Diego, I., Wilmanns, M. & Schultz, C. ACS Chem Biol. 2011 Jul 15;6(7):685-91. Epub 2011 Apr 28. PubMed

The ENaC-overexpressing mouse as a model of cystic fibrosis lung disease. Zhou, Z., Duerr, J., Johannesson, B., Schubert, S.C., Treis, D., Harm, M., Graeber, S.Y., Dalpke, A., Schultz, C. & Mall, M.A. J Cyst Fibros. 2011 Jun;10 Suppl 2:S172-82. PubMed

Genetically encoded copper-free click chemistry. Plass, T., Milles, S., Koehler, C., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2011 Apr 18;50(17):3878-81. doi:10.1002/anie.201008178. Epub 2011 Mar 23. PubMed

Photoactivatable and cell-membrane-permeable phosphatidylinositol 3,4,5-trisphosphate. Mentel, M., Laketa, V., Subramanian, D., Gillandt, H. & Schultz, C. Angew Chem Int Ed Engl. 2011 Apr 11;50(16):3811-4. doi:10.1002/anie.201007796. Epub 2011 Mar 14. PubMed

Imaging probes--Introduction. Schultz, C. & Aime, S. Bioorg Med Chem. 2011 Feb 1;19(3):1022. PubMed

Does cellular hydrogen peroxide diffuse or act locally? Mishina, N.M., Tyurin-Kuzmin, P.A., Markvicheva, K.N., Vorotnikov, A.V., Tkachuk, V.A., Laketa, V., Schultz, C., Lukyanov, S. & Belousov, V.V. Antioxid Redox Signal. 2011 Jan 1;14(1):1-7. Epub 2010 Oct 12. PubMed

Covalent Labeling of Biomolecules in Living Cells. Plass, T. and Schultz, C. Advanced Fluorescence Reporters in Chemistry and Biology III: Applications to Sensing and Imaging. A. P. Demchenko, (Ed.); Springer Series on Fluorescence, vol. 10. (O. S. Wolfbeis, Series Ed.); ISBN: 978-3-642-18034-7.
Link

Inositol pentakisphosphate isomers bind PH domains with varying specificity and inhibit phosphoinositide interactions. Jackson, S.G., Al-Saigh, S., Schultz, C. & Junop, M.S. BMC Struct Biol. 2011 Feb 10;11:11. PubMed

Airway surface liquid volume regulation determines different airway phenotypes in liddle compared with betaENaC-overexpressing mice. Mall, M.A., Button, B., Johannesson, B., Zhou, Z., Livraghi, A., Caldwell, R.A., Schubert, S.C., Schultz, C., O'Neal, W.K., Pradervand, S., Hummler, E., Rossier, B.C., Grubb, B.R. & Boucher, R.C. J Biol Chem. 2010 Aug 27;285(35):26945-55. Epub 2010 Jun 21. PubMed

Challenges in studying phospholipid signaling. Schultz, C. Nat Chem Biol. 2010 Jul;6(7):473-5. PubMed

Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells. Subramanian, D.*, Laketa, V.*, Muller, R., Tischer, C., Zarbakhsh, S., Pepperkok, R. & Schultz, C. * - equally contributing first authors. Nat Chem Biol. 2010 May;6(5):324-6. Epub 2010 Apr 4. PubMed

The ßENaC-overexpressing mouse as a model of cystic fibrosis lung disease. Zhou, Z., Duerr, J., Johannesson, B., Schubert, S. C., Treis, D., Harm, M., Gräber, S., Dalpke, A., Schultz, C., Mall, M. A. J. Cystic Fibr. (2010). In press

Imaging lipids in living cells. Schultz, C., Neef, A., Goedhart, J. & Gadella, T.W.J. In 'Live Cell Imaging', Spector, D.L., Svedlow, J. & Jaron, B.F (eds), 2010, Cold Spring Harbor Press

Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth. Laketa, V., Zarbakhsh, S., Morbier, E., Subramanian, D., Dinkel, C., Brumbaugh, J., Zimmermann, P., Pepperkok, R. & Schultz, C. Chem Biol. 2009 Nov 25;16(11):1190-6. PubMed

Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation. Cobos-Correa, A., Trojanek, J.B., Diemer, S., Mall, M.A. & Schultz, C. Nat Chem Biol. 2009 Sep;5(9):628-30. Epub 2009 Aug 2. PubMed

Fluorescent revelations. Schultz, C. Chem Biol. 2009 Feb 27;16(2):107-11. PubMed

Chemical biologists gather in Heidelberg. Köhn, M. & Schultz, C. Nat Chem Biol. 2009 Feb;5(2):66-9. PubMed

Selective fluorescence labeling of lipids in living cells. Neef, A.B. & Schultz, C. Angew Chem Int Ed Engl. 2009;48(8):1498-500. PubMed

Analysis of protein complex hierarchy in living cells. Piljic, A. & Schultz, C. ACS Chem Biol. 2008 Dec 19;3(12):749-55. PubMed

Contribution of fluorophores to protein kinase C FRET probe performance. Jost, C.A., Reither, G., Hoffmann, C. & Schultz, C. Chembiochem. 2008 Jun 16;9(9):1379-84. PubMed

Simultaneous recording of multiple cellular events by FRET. Piljic, A. & Schultz, C. ACS Chem Biol. 2008 Mar 20;3(3):156-60. PubMed

Heterogeneity and timing of translocation and membrane-mediated assembly of different annexins. Skrahina, T., Piljic, A. & Schultz, C. Exp Cell Res. 2008 Mar 10;314(5):1039-47. Epub 2007 Nov 29. PubMed

Fluorescence and bioluminescence procedures for functional proteomics. Prinz, A., Reither, G., Diskar, M. & Schultz, C. Proteomics. 2008 Mar;8(6):1179-96. PubMed

Probing lipid- and drug-binding domains with fluorescent dyes. Black, S.L., Stanley, W.A., Filipp, F.V., Bhairo, M., Verma, A., Wichmann, O., Sattler, M., Wilmanns, M. & Schultz, C. Bioorg Med Chem. 2008 Feb 1;16(3):1162-73. Epub 2007 Nov 19. PubMed

Simultaneous protein tagging in two colors. Schultz, C. & Köhn, M. Chem Biol. 2008 Feb;15(2):91-2. PubMed

Small molecule-based FRET probes. Cobos Correa, A. & Schultz, C. In "FLIM and FRET", Gadella, T. (ed.), Elsevier, 2008, pp 225-288

Molecular tools for cell and systems biology Schultz, C. Human Frontiers Sci. Progr. J. 2007/11 1(4) 230-248

Probing phospholipase a(2) with fluorescent phospholipid substrates. Wichmann, O., Gelb, M.H. & Schultz, C. Chembiochem. 2007 Sep 3;8(13):1555-69. PubMed

Investigation of the ligand spectrum of human sterol carrier protein 2 using a direct mass spectrometry assay. Stanley, W.A., Versluis, K., Schultz, C., Heck, A.J. & Wilmanns, M. Arch Biochem Biophys. 2007 May 1;461(1):50-8. Epub 2007 Mar 15. PubMed

Structural analysis of the binding of myo-inositol pentakisphosphates by the C-terminal PH domain of pleckstrin. Jackson, SG; Zhang, Y; Zhang, K; Bao, XK; Schultz, C.; Haslam, R; Junop, M FASEB Journal April 2007 21(5)

Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B. Yudushkin, I.A., Schleifenbaum, A., Kinkhabwala, A., Neel, B.G., Schultz, C. & Bastiaens, P.I. Science. 2007 Jan 5;315(5808):115-9. PubMed

Controlling protein function by caged compounds. Giordano, A., Zarbahksh, S. & Schultz, C. In "Chemical Biology" S. Schreiber, T. Kapoor, G. Wess (eds.), Wiley/VCH, 2007, pp140-173

Lipid-induced phenotypes. Schultz, C. Nat Chem Biol. 2006 Aug;2(8):396-8. PubMed

Annexin a4 self-association modulates general membrane protein mobility in living cells. Piljic, A. & Schultz, C. Mol Biol Cell. 2006 Jul;17(7):3318-28. Epub 2006 May 10. PubMed

Cellular uptake of PNA-terpyridine conjugates and its enhancement by Zn(2+) Ions. Füssl, A., Schleifenbaum, A., Goritz, M., Riddell, A., Schultz, C. & Kramer, R. J Am Chem Soc. 2006 May 10;128(18):5986-7. PubMed

Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells. Tabary, O., Boncoeur, E., de Martin, R., Pepperkok, R., Clement, A., Schultz, C. & Jacquot, J. Cell Signal. 2006 May;18(5):652-60. Epub 2005 Aug 9. PubMed

A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms. Wichmann, O., Wittbrodt, J. & Schultz, C. Angew Chem Int Ed Engl. 2006 Jan 9;45(3):508-12. PubMed

A dual parameter FRET probe for measuring PKC and PKA activity in living cells. Brumbaugh, J., Schleifenbaum, A., Gasch, A., Sattler, M. & Schultz, C. J Am Chem Soc. 2006 Jan 11;128(1):24-5. PubMed

An inositol polyphosphate derivative inhibits Na+ flux and improves fluid dynamics in cystic fibrosis airway epithelia. Moody, M., Pennington, C., Schultz, C., Caldwell, R., Dinkel, C., Rossi, M.W., McNamara, S., Widdicombe, J., Gabriel, S. & Traynor-Kaplan, A.E. Am J Physiol Cell Physiol 2005 Sep;289(3):C512-20. Epub 2005 Apr 27. PubMed

Multiparameter imaging for the analysis of intracellular signaling. Schultz, C., Schleifenbaum, A., Goedhart, J. & Gadella TW, J.r Chembiochem 2005 Aug;6(8):1323-30. PubMed

Closing the gap between chemistry and biology. Scharer, O. & Schultz, C. Chembiochem 2005 Jan;6(1):3-5. PubMed

A genetically encoded FRET probe for PKC activity based on pleckstrin. Schleifenbaum, A., Stier, G., Gasch, A., Sattler, M. & Schultz, C. J Am Chem Soc 2004 Sep 29;126(38):11786-7. PubMed

Antagonists of myo-inositol 3,4,5,6-tetrakisphosphate allow repeated epithelial chloride secretion. Rudolf, M.T., Dinkel, C., Traynor-Kaplan, A.E. & Schultz, C. Bioorg Med Chem 2003 Jul 31;11(15):3315-29. PubMed

Prodrugs of biologically active phosphate esters. Schultz, C. Bioorg Med Chem 2003 Mar 20;11(6):885-98. PubMed

Versatile reagents to introduce caged phosphates. Dinkel, C., Wichmann, O., Schultz, C. Tetrahedron Lett. 2003 (44) 1153-1155

Synthesis of caged myo-inositol 1,3,4,5-tetrakisphosphate. Dinkel, C., Schultz, C. Tetrahedron Lett. 2003 (44) 1157-1159

Biocatalysis and Enzyme-Analogues Processes