Mattaj GroupC. elegans BAF-VRK Movies
Publications:
Gorjanacz M, Klerkx EP, Galy V, Santarella R, Lopez-Iglesias C, Askjaer P, Mattaj IW. Caenorhabditis elegans BAF-1 and its kinase VRK-1 participate directly in post-mitotic nuclear envelope assembly. EMBO J. 2007 Jan 10; 26(1): 132-43.
Gorjanacz M, Mattaj IW, Rietdorf J. Some like it hot: Switching biology on the microscope. G.I.T. Imaging & Microscopy 2007 March 23; 9: 28-29.
Movies corresponding to Figure 1D:
Video 1. Time-lapse confocal recording of wild type embryo expressing YFP-LMN-1 at 25°C. In this and all subsequent videos embryos are oriented with anterior to the left and time is indicated in seconds relative to onset of anaphase I [0 sec]. Playback speed of all videos is 60x.
Video 2. Confocal time-lapse recording of baf-1[t1639] embryo expressing YFP-LMN-1 at 25°C.
Movies corresponding to Figure 3A:
Video 3. Confocal time-lapse recording of control embryo expressing GFP-β-tubulin at 15°C.
Video 4. Confocal time-lapse recording of baf-1[RNAi] embryo expressing GFP-β-tubulin at 25°C. BAF-1 RNAi was performed for 48 h.
Video 5. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin at 15°C.
Video 6. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin at 25°C.
Movies corresponding to Figure 3B:
Video 7. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin. The embryo was produced and recorded at 15°C, upshifted to 25°C at pronuclear stage, -250 sec before anaphase onset, and downshifted to 15°C at anaphase onset [0 sec].
Video 8. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin. The embryo was produced and recorded at 15°C, upshifted to 25°C at anaphase onset [0 sec], and downshifted to 15°C 250 sec after.
Video 9. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin. The embryo was produced and recorded at 15°C, and upshifted to 25°C 80 sec after anaphase onset.
Video 10. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-β-tubulin. The embryo was produced and recorded at 15°C, and upshifted to 25°C 160 sec after anaphase onset.
Movies corresponding to Figure 3G:
Video 11. Confocal time-lapse recording of wild type embryo expressing GFP-LEM-2 at 15°C.
Video 12. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-LEM-2 at 15°C.
Video 13. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-LEM-2. The embryo was produced and recorded at 15°C, and upshifted to 25°C 80 sec after anaphase onset.
Video 14. Confocal time-lapse recording of baf-1[t1639] embryo expressing GFP-LEM-2. The embryo was produced and recorded at 15°C, and upshifted to 25°C 280 sec after anaphase onset.
Movies corresponding to Figure 5A:
Video 15. Confocal time-lapse recording of control [upper] and vrk-1[RNAi] [lower] embryos expressing YFP-LMN-1. VRK-1 RNAi was performed for 24 h.
Movie corresponding to the Figure 6A:
Video 16. Confocal time-lapse recording of wild type embryo expressing GFP-VRK-1.
Movies corresponding to the Figure 6B:
Video 17. Confocal time-lapse recording of control embryo expressing GFP-BAF-1.
Video 18. Confocal time-lapse recording of vrk-1[RNAi] embryo expressing GFP-BAF-1. VRK-1 RNAi was performed for 13 h.
Video 19. Confocal time-lapse recording of ran-1[RNAi] embryo expressing GFP-BAF-1. RAN-1 RNAi was performed for 16 h.
Video 20. Confocal time-lapse recording of wild type embryo expressing GFP-BAF-1 G38D at 25°C.
Movies corresponding to the Figure 7A:
Video 21. Confocal time-lapse recording of control [upper] and vrk-1[RNAi] [lower] embryos expressing GFP-LEM-2. VRK-1 RNAi was performed for 24 h.
Movies corresponding to the Supplementary Figure 2:
Video 22. Spinning disk time-lapse recording of control embryo co-expressing GFP-histone H2B and GFP-β-tubulin.
Video 23. Spinning disk time-lapse recording of baf-1[RNAi] embryo co-expressing GFP-histone H2B and GFP-β-tubulin. BAF-1 RNAi was performed for 48 h.
Movies corresponding to the Supplementary Figure 5A:
Video 24. Confocal time-lapse recording of control [upper] and vrk-1[RNAi] [lower] embryos expressing YFP-Nup-107. VRK-1 RNAi was performed for 24 h.
Movies corresponding to the Supplementary Figure 5B:
Video 25. Confocal time-lapse recording of control [upper] and vrk-1[RNAi] [lower] embryos expressing GFP-Nup35. VRK-1 RNAi was performed for 24 h.
Movies corresponding to the Supplementary Figure 6:
Video 26. Confocal time-lapse recording of control [upper] and vrk-1[RNAi] [lower] embryos expressing GFP-histone H2B. VRK-1 RNAi was performed for 24 h.