Staff at EMBL
Dr. Jeroen Krijgsveld
Team Leader and Head of Proteomic Core Facility
Krijgsveld Team
Proteomic Core Facility
EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
E-mail:
Biography
PhD 1999, University of Amsterdam, The Netherlands
Postdoc at Utrecht University, The Netherlands and Harvard Medical School, Boston, USA
Assistant Professor, Utrecht University, The Netherlands
Team Leader at EMBL since October 2008
Publications
Phosphorylation dynamics during early differentiation of human embryonic stem cells. Van Hoof, D., Munoz, J., Braam, S.R., Pinkse, M.W., Linding, R., Heck, A.J., Mummery, C.L. & Krijgsveld, J. Cell Stem Cell. 2009 Aug 7;5(2):214-26. PubMed
Orthogonal separation techniques for the characterization of the yeast nuclear proteome. Gauci, S., Veenhoff, L.M., Heck, A.J. & Krijgsveld, J. J Proteome Res. 2009 Jul;8(7):3451-63. PubMed
An AC-5 cathepsin B-like protease purified from Haemonchus contortus excretory secretory products shows protective antigen potential for lambs. De Vries, E., Bakker, N., Krijgsveld, J., Knox, D.P., Heck, A.J. & Yatsuda, A.P. Vet Res. 2009 Jul-Aug;40(4):41. Epub 2009 Apr 30. PubMed
Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Gauci, S., Helbig, A.O., Slijper, M., Krijgsveld, J., Heck, A.J. & Mohammed, S. Anal Chem. 2009 Jun 1;81(11):4493-501. PubMed
Novel Hsp90 partners discovered using complementary proteomic approaches. Tsaytler, P.A., Krijgsveld, J., Goerdayal, S.S., Rudiger, S. & Egmond, M.R. Cell Stress Chaperones. 2009 Apr 26. PubMed
In vivo stable isotope labeling of fruit flies reveals post-transcriptional regulation in the maternal-to-zygotic transition. Gouw, J.W., Pinkse, M.W., Vos, H.R., Moshkin, Y., Verrijzer, C.P., Heck, A.J. & Krijgsveld, J. Mol Cell Proteomics. 2009 Mar 24. PubMed
Exosomal secretion of cytoplasmic prostate cancer xenograft-derived proteins. Jansen, F.H., Krijgsveld, J., van Rijswijk, A., van den Bemd, G.J., van den Berg, M.S., van Weerden, W.M., Willemsen, R., Dekker, L.J., Luider, T.M. & Jenster, G. Mol Cell Proteomics. 2009 Feb 9. PubMed
Human Proteome Organisation's 7th World Congress, 2008. Krijgsveld, J. Expert Rev Proteomics. 2008 Dec;5(6):775-7. PubMed
A versatile peptide pI calculator for phosphorylated and N-terminal acetylated peptides experimentally tested using peptide isoelectric focusing. Gauci, S., van Breukelen, B., Lemeer, S.M., Krijgsveld, J. & Heck, A.J. Proteomics. 2008 Dec;8(23-24):4898-906. PubMed
Feeder-free monolayer cultures of human embryonic stem cells express an epithelial plasma membrane protein profile. Van Hoof, D., Braam, S.R., Dormeyer, W., Ward-van Oostwaard, D., Heck, A.J., Krijgsveld, J. & Mummery, C.L. Stem Cells. 2008 Nov;26(11):2777-81. Epub 2008 Aug 14. PubMed
Optimizing identification and quantitation of 15N-labeled proteins in comparative proteomics. Gouw, J.W., Tops, B.B., Mortensen, P., Heck, A.J. & Krijgsveld, J. Anal Chem. 2008 Oct 15;80(20):7796-803. Epub 2008 Sep 23. PubMed
A practical guide for the identification of membrane and plasma membrane proteins in human embryonic stem cells and human embryonal carcinoma cells. Dormeyer, W., van Hoof, D., Mummery, C.L., Krijgsveld, J. & Heck, A.J. Proteomics. 2008 Oct;8(19):4036-53. PubMed
Proteomics and human embryonic stem cells. Van Hoof, D., Heck, A.J., Krijgsveld, J. & Mummery, C.L. Stem Cell Res. 2008 Sep;1(3):169-82. Epub 2008 May 29. PubMed
Immediate protein targets of photodynamic treatment in carcinoma cells. Tsaytler, P.A., C O'Flaherty, M., Sakharov, D.V., Krijgsveld, J. & Egmond, M.R. J Proteome Res. 2008 Sep;7(9):3868-78. Epub 2008 Jul 25. PubMed
Plasma membrane proteomics of human embryonic stem cells and human embryonal carcinoma cells. Dormeyer, W., van Hoof, D., Braam, S.R., Heck, A.J., Mummery, C.L. & Krijgsveld, J. J Proteome Res. 2008 Jul;7(7):2936-51. Epub 2008 May 20. PubMed
Nonprocessive methylation by Dot1 leads to functional redundancy of histone H3K79 methylation states. Frederiks, F., Tzouros, M., Oudgenoeg, G., van Welsem, T., Fornerod, M., Krijgsveld, J. & van Leeuwen, F. Nat Struct Mol Biol. 2008 Jun;15(6):550-7. Epub 2008 May 30. PubMed
The time is right: proteome biology of stem cells. Whetton, A.D., Williamson, A.J., Krijgsveld, J., Lee, B.H., Lemischka, I., Oh, S., Pera, M., Mummery, C. & Heck, A.J. Cell Stem Cell. 2008 Mar 6;2(3):215-7. PubMed
Proteome biology of stem cells: a new joint HUPO and ISSCR initiative. Krijgsveld, J., Whetton, A.D., Lee, B., Lemischka, I., Oh, S., Pera, M., Mummery, C. & Heck, A.J. Mol Cell Proteomics. 2008 Jan;7(1):204-5. PubMed
Targeted analysis of protein termini. Dormeyer, W., Mohammed, S., Breukelen, B., Krijgsveld, J. & Heck, A.J. J Proteome Res. 2007 Dec;6(12):4634-45. Epub 2007 Oct 10. PubMed
Proteome biology of stem cells. Heck, A.J., Mummery, C., Whetton, A., Oh, S., Lee, B., Pera, M., Lemischka, I. & Krijgsveld, J. Stem Cell Res. 2007 Oct;1(1):7-8. Epub 2007 Aug 24. PubMed
An experimental correction for arginine-to-proline conversion artifacts in SILAC-based quantitative proteomics. Van Hoof, D., Pinkse, M.W., Oostwaard, D.W., Mummery, C.L., Heck, A.J. & Krijgsveld, J. Nat Methods. 2007 Sep;4(9):677-8. PubMed
A high-quality catalog of the Drosophila melanogaster proteome. Brunner, E., Ahrens, C.H., Mohanty, S., Baetschmann, H., Loevenich, S., Potthast, F., Deutsch, E.W., Panse, C., de Lichtenberg, U., Rinner, O., Lee, H., Pedrioli, P.G., Malmstrom, J., Koehler, K., Schrimpf, S., Krijgsveld, J., Kregenow, F., Heck, A.J., Hafen, E., Schlapbach, R. & Aebersold, R. Nat Biotechnol. 2007 May;25(5):576-83. Epub 2007 Apr 22. PubMed
Novel binding partners of Ldb1 are required for haematopoietic development. Meier, N., Krpic, S., Rodriguez, P., Strouboulis, J., Monti, M., Krijgsveld, J., Gering, M., Patient, R., Hostert, A. & Grosveld, F. Development. 2006 Dec;133(24):4913-23. Epub 2006 Nov 15. PubMed
Mass spectrometric identification of human prostate cancer-derived proteins in serum of xenograft-bearing mice. van den Bemd, G.J., Krijgsveld, J., Luider, T.M., van Rijswijk, A.L., Demmers, J.A. & Jenster, G. Mol Cell Proteomics. 2006 Oct;5(10):1830-9. Epub 2006 May 19. PubMed
Embryonic stem cell proteomics. Van Hoof, D., Mummery, C.L., Heck, A.J. & Krijgsveld, J. Expert Rev Proteomics. 2006 Aug;3(4):427-37. PubMed
A quest for human and mouse embryonic stem cell-specific proteins. Van Hoof, D., Passier, R., Ward-Van Oostwaard, D., Pinkse, M.W., Heck, A.J., Mummery, C.L. & Krijgsveld, J. Mol Cell Proteomics. 2006 Jul;5(7):1261-73. Epub 2006 Apr 6. PubMed
In-gel isoelectric focusing of peptides as a tool for improved protein identification. Krijgsveld, J., Gauci, S., Dormeyer, W. & Heck, A.J. J Proteome Res. 2006 Jul;5(7):1721-30. PubMed
Identification of secreted cysteine proteases from the parasitic nematode Haemonchus contortus detected by biotinylated inhibitors. Yatsuda, A.P., Bakker, N., Krijgsveld, J., Knox, D.P., Heck, A.J. & de Vries, E. Infect Immun. 2006 Mar;74(3):1989-93. PubMed
GATA-1 forms distinct activating and repressive complexes in erythroid cells. Rodriguez, P., Bonte, E., Krijgsveld, J., Kolodziej, K.E., Guyot, B., Heck, A.J., Vyas, P., de Boer, E., Grosveld, F. & Strouboulis, J. EMBO J. 2005 Jul 6;24(13):2354-66. Epub 2005 May 26. PubMed
Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome. van Balkom, B.W., van Gestel, R.A., Brouwers, J.F., Krijgsveld, J., Tielens, A.G., Heck, A.J. & van Hellemond, J.J. J Proteome Res. 2005 May-Jun;4(3):958-66. PubMed
GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7. van der Knaap, J.A., Kumar, B.R., Moshkin, Y.M., Langenberg, K., Krijgsveld, J., Heck, A.J., Karch, F. & Verrijzer, C.P. Mol Cell. 2005 Mar 4;17(5):695-707. PubMed
Munc13-4 is an effector of rab27a and controls secretion of lysosomes in hematopoietic cells. Neeft, M., Wieffer, M., de Jong, A.S., Negroiu, G., Metz, C.H., van Loon, A., Griffith, J., Krijgsveld, J., Wulffraat, N., Koch, H., Heck, A.J., Brose, N., Kleijmeer, M. & van der Sluijs, P. Mol Biol Cell. 2005 Feb;16(2):731-41. Epub 2004 Nov 17. PubMed
Mass spectrometry-based quantitative proteomics. Heck, A.J. & Krijgsveld, J. Expert Rev Proteomics. 2004 Oct;1(3):317-26. PubMed
Quantitative cancer proteomics: stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research. Everley, P.A., Krijgsveld, J., Zetter, B.R. & Gygi, S.P. Mol Cell Proteomics. 2004 Jul;3(7):729-35. Epub 2004 Apr 21. PubMed
Differential targeting of two distinct SWI/SNF-related Drosophila chromatin-remodeling complexes. Mohrmann, L., Langenberg, K., Krijgsveld, J., Kal, A.J., Heck, A.J. & Verrijzer, C.P. Mol Cell Biol. 2004 Apr;24(8):3077-88. PubMed
Lung proteome alterations in a mouse model for nonallergic asthma. Houtman, R., Krijgsveld, J., Kool, M., Romijn, E.P., Redegeld, F.A., Nijkamp, F.P., Heck, A.J. & Humphery-Smith, I. Proteomics. 2003 Oct;3(10):2008-18. PubMed
Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics. Krijgsveld, J., Ketting, R.F., Mahmoudi, T., Johansen, J., Artal-Sanz, M., Verrijzer, C.P., Plasterk, R.H. & Heck, A.J. Nat Biotechnol. 2003 Aug;21(8):927-31. Epub 2003 Jul 13. PubMed
Recent liquid chromatographic-(tandem) mass spectrometric applications in proteomics. Romijn, E.P., Krijgsveld, J. & Heck, A.J. J Chromatogr A. 2003 Jun 6;1000(1-2):589-608. PubMed
Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. de Boer, E., Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., Grosveld, F. & Strouboulis, J. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5. Epub 2003 Jun 11. PubMed
Comprehensive analysis of the secreted proteins of the parasite Haemonchus contortus reveals extensive sequence variation and differential immune recognition. Yatsuda, A.P., Krijgsveld, J., Cornelissen, A.W., Heck, A.J. & de Vries, E. J Biol Chem. 2003 May 9;278(19):16941-51. Epub 2003 Feb 7. PubMed