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Staff at EMBL

Dr. Orsolya Barabas

Group Leader
Barabas Group

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Tel: +49 6221 387-8626
E-mail: orsolya.barabas@embl.de

Biography

PhD 2005, Eötvös Loránd University, Budapest, Hungary.
Postdoctoral research at the National Institutes of Health, Bethesda, USA.
Group leader at EMBL since 2009.

Publications

Acquisition of an Archaea-like ribonuclease H domain by plant L1 retrotransposons supports modular evolution. Smyshlyaev, G., Voigt, F., Blinov, A., Barabas, O. & Novikova, O. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20140-5. doi:10.1073/pnas.1310958110. Epub 2013 Nov 25. Europe PMC

Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling. Barabas, O., Nemeth, V., Bodor, A., Perczel, A., Rosta, E., Kele, Z., Zagyva, I., Szabadka, Z., Grolmusz, V.I., Wilmanns, M. & Vertessy, B.G. Nucleic Acids Res. 2013 Dec;41(22):10542-55. doi: 10.1093/nar/gkt756. Epub 2013Aug 27. Europe PMC

Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc. Voigt, F., Reuter, M., Kasaruho, A., Schulz, E.C., Pillai, R.S. & Barabas, O. RNA. 2012 Dec;18(12):2128-34. doi: 10.1261/rna.034967.112. Epub 2012 Oct 19. Europe PMC

DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans. Hickman, A.B., James, J.A., Barabas, O., Pasternak, C., Ton-Hoang, B., Chandler, M., Sommer, S. & Dyda, F. EMBO J. 2010 Nov 17;29(22):3840-52. Epub 2010 Oct 1. Europe PMC

Resetting the site: redirecting integration of an insertion sequence in a predictable way. Guynet, C., Achard, A., Hoang, B.T., Barabas, O., Hickman, A.B., Dyda, F. & Chandler, M. Mol Cell. 2009 Jun 12;34(5):612-9. Europe PMC

Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers. Takacs, E., Barabas, O., Petoukhov, M.V., Svergun, D.I. & Vertessy, B.G. FEBS Lett. 2009 Mar 4;583(5):865-71. Epub 2009 Feb 11. Europe PMC

Helicobacter Pylori's plasticity zones are novel transposable elements. Kersulyte, D., Lee, W., Subramaniam, D., Anant, S., Herrera, P., Cabrera, L., Balqui, J., Barabas, O., Kalia, A., Gilman, R.H. & Berg, D.E. PLoS One. 2009 Sep 3;4(9):e6859. Europe PMC

Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor. Varga, B., Barabas, O., Takacs, E., Nagy, N., Nagy, P. & Vertessy, B.G. Biochem Biophys Res Commun. 2008 Aug 15;373(1):8-13. Epub 2008 Jun 2. Europe PMC

Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site. Kovari, J., Barabas, O., Varga, B., Bekesi, A., Tolgyesi, F., Fidy, J., Nagy, J. & Vertessy, B.G. Proteins. 2008 Apr;71(1):308-19. Europe PMC

Revisiting the mechanism of the autoactivation of the complement protease C1r in the C1 complex: structure of the active catalytic region of C1r. Kardos, J., Harmat, V., Pallo, A., Barabas, O., Szilagyi, K., Graf, L., Naray-Szabo, G., Goto, Y., Zavodszky, P. & Gal, P. Mol Immunol. 2008 Mar;45(6):1752-60. Epub 2007 Nov 9. Europe PMC

In vitro reconstitution of a single-stranded transposition mechanism of IS608. Guynet, C., Hickman, A.B., Barabas, O., Dyda, F., Chandler, M. & Ton-Hoang, B. Mol Cell. 2008 Feb 15;29(3):302-12. Europe PMC

Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection. Barabas, O., Ronning, D.R., Guynet, C., Hickman, A.B., Ton-Hoang, B., Chandler, M. & Dyda, F. Cell. 2008 Jan 25;132(2):208-20. Europe PMC

Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. Varga, B., Barabas, O., Kovari, J., Toth, J., Hunyadi-Gulyas, E., Klement, E., Medzihradszky, K.F., Tolgyesi, F., Fidy, J. & Vertessy, B.G. FEBS Lett. 2007 Oct 2;581(24):4783-8. Epub 2007 Sep 12. Europe PMC

Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins. Nemeth-Pongracz, V., Barabas, O., Fuxreiter, M., Simon, I., Pichova, I., Rumlova, M., Zabranska, H., Svergun, D., Petoukhov, M., Harmat, V., Klement, E., Hunyadi-Gulyas, E., Medzihradszky, K.F., Konya, E. & Vertessy, B.G. Nucleic Acids Res. 2007;35(2):495-505. Epub 2006 Dec 14. Europe PMC

Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus. Barabas, O., Nemeth, V. & Vertessy, B.G. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt4):399-401. Epub 2006 Mar 25. Europe PMC

Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase. Barabas, O., Pongracz, V., Kovari, J., Wilmanns, M. & Vertessy, B.G. J Biol Chem 2004 Oct 8;279(41):42907-15. Epub 2004 Jun 17. Europe PMC

Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme. Kovari, J., Barabas, O., Takacs, E., Bekesi, A., Dubrovay, Z., Pongracz, V., Zagyva, I., Imre, T., Szabo, P. & Vertessy, B.G. J Biol Chem. 2004 Apr 23;279(17):17932-44. Epub 2004 Jan 14. Europe PMC

dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency. Barabas, O., Rumlova, M., Erdei, A., Pongracz, V., Pichova, I. & Vertessy, B.G. J Biol Chem. 2003 Oct 3;278(40):38803-12. Epub 2003 Jul 16. Europe PMC

Synthesis and stereochemistry of dispiro substituted pyridazines: application of ellipticity-absorbance ratio spectra for proving enantiomeric relationship by HPLC-CD/UV detection. Beke, G., Gergely, A., Szasz, G., Szentesi, A., Nyitray, J., Barabas, O., Harmath, V. & Matyus, P. Chirality. 2002 May 15;14(5):365-71. Europe PMC

Theoretical and experimental studies on ring closure reactions of 4(5)-chloro-5(4)-hydroxyalkylamino-6-nitro-3-(2H)-pyridazinones. Éliás, O., Károlyházy, L., Stájer, G., Fülöp, F., Czakó, K., Harmat, V., Barabás, O., Keserű, K., Mátyus, P. J. Mol. Structure – Theochem. 2001;545:75-96

Novel ring transformations of condensed [1,2,4]triazolo[4,3-b]pyridazine-6(5H)-one-3(2H)-thiones effected by dialkyl-acetylenedicarboxylates. Simó, M., Csámpai, A., Harmat, V., Barabás, O., Magyarfalvy, G. Tetrahedron. 2009;57:7191-7198

Study on ferrocenes, part 9 [1] substrate selective transformations of some ferrocenylhydrazones. Abrán, Á., Csámpai, A., Kotschy, A., Barabás, O., Sohár, P. J. Mol. Structure. 2001;569:185-194

Structural basis of solid solution formation during chiral resolution. Barabás, O., Menyhárd, D.K., Böcskei, Zs., Simon, K., Kiss-Ajzert, I., Takács K. and Hermecz I. Tetrahedron: Asymmetry. 2000;11:4061-4070