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Staff at EMBL

CP-60004626

Dr. Orsolya Barabas

Group Leader
Barabas Group

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Tel: +49 6221 387-8626
E-mail: orsolya.barabas@embl.de

Biography

PhD 2005, Eötvös Loránd University, Budapest, Hungary.
Postdoctoral research at the National Institutes of Health, Bethesda, USA.
Group leader at EMBL since 2009.

Publications

Total: 25 publication(s)

2014

Bock T, Chen WH, Ori A, Malik N, Silva-Martin N, Huerta-Cepas J, Powell ST, Kastritis PL, Smyshlyaev G, Vonkova I, Kirkpatrick J, Doerks T, Nesme L, Baßler J, Kos M, Hurt E, Carlomagno T, Gavin AC, Barabas O, Müller CW, van Noort V, Beck M, Bork P. (2014)
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum.
Nucleic Acids Res. doi:10.1093/nar/gku1147
Europe PMC | doi


2013

Smyshlyaev G, Voigt F, Blinov A, Barabas O, Novikova O. (2013)
Acquisition of an Archaea-like ribonuclease H domain by plant L1 retrotransposons supports modular evolution
Proc. Natl. Acad. Sci. U.S.A. 110(50):20140-20145. doi:10.1073/pnas.1310958110
Europe PMC | doi

Barabás O, Németh V, Bodor A, Perczel A, Rosta E, Kele Z, Zagyva I, Szabadka Z, Grolmusz VI, Wilmanns M, Vértessy BG. (2013)
Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling
Nucleic Acids Res. 41(22):10542-10555. doi:10.1093/nar/gkt756
Europe PMC | doi


2012

Voigt F, Reuter M, Kasaruho A, Schulz EC, Pillai RS, Barabas O. (2012)
Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc
RNA 18(12):2128-2134. doi:10.1261/rna.034967.112
Europe PMC | doi

Barabas O. (2012)
Mechanism of Single-Stranded DNA Transposition: A Structural Perspective.
In: Wiley Online Library, eLS. John Wiley & Sons Ltd:, Chichester.doi:10.1002/9780470015902.a0023178
doi


2010

Hickman AB, James JA, Barabas O, Pasternak C, Ton-Hoang B, Chandler M, Sommer S, Dyda F. (2010)
DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans
EMBO J. 29(22):3840-3852. doi:10.1038/emboj.2010.241
Europe PMC | doi


2009

Guynet C, Achard A, Hoang BT, Barabas O, Hickman AB, Dyda F, Chandler M. (2009)
Resetting the site: redirecting integration of an insertion sequence in a predictable way
Mol. Cell 34(5):612-619. doi:10.1016/j.molcel.2009.05.017
Europe PMC | doi

Takács E, Barabás O, Petoukhov MV, Svergun DI, Vértessy BG. (2009)
Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers
FEBS Lett. 583(5):865-871. doi:10.1016/j.febslet.2009.02.011
Europe PMC | doi

Kersulyte D, Lee W, Subramaniam D, Anant S, Herrera P, Cabrera L, Balqui J, Barabas O, Kalia A, Gilman RH, Berg DE. (2009)
Helicobacter Pylori's plasticity zones are novel transposable elements
PLoS ONE 4(9):e6859. doi:10.1371/journal.pone.0006859
Europe PMC | doi


2008

Varga B, Barabás O, Takács E, Nagy N, Nagy P, Vértessy BG. (2008)
Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor
Biochem. Biophys. Res. Commun. 373(1):8-13. doi:10.1016/j.bbrc.2008.05.130
Europe PMC | doi

Kovári J, Barabás O, Varga B, Békési A, Tölgyesi F, Fidy J, Nagy J, Vértessy BG. (2008)
Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site
Proteins 71(1):308-319. doi:10.1002/prot.21757
Europe PMC | doi

Kardos J, Harmat V, Palló A, Barabás O, Szilágyi K, Gráf L, Náray-Szabó G, Goto Y, Závodszky P, Gál P. (2008)
Revisiting the mechanism of the autoactivation of the complement protease C1r in the C1 complex: structure of the active catalytic region of C1r
Mol. Immunol. 45(6):1752-1760. doi:10.1016/j.molimm.2007.09.031
Europe PMC | doi

Guynet C, Hickman AB, Barabas O, Dyda F, Chandler M, Ton-Hoang B. (2008)
In vitro reconstitution of a single-stranded transposition mechanism of IS608
Mol. Cell 29(3):302-312. doi:10.1016/j.molcel.2007.12.008
Europe PMC | doi

Barabas O, Ronning DR, Guynet C, Hickman AB, Ton-Hoang B, Chandler M, Dyda F. (2008)
Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection
Cell 132(2):208-220. doi:10.1016/j.cell.2007.12.029
Europe PMC | doi


2007

Varga B, Barabás O, Kovári J, Tóth J, Hunyadi-Gulyás E, Klement E, Medzihradszky KF, Tölgyesi F, Fidy J, Vértessy BG. (2007)
Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase
FEBS Lett. 581(24):4783-4788. doi:10.1016/j.febslet.2007.09.005
Europe PMC | doi

Németh-Pongrácz V, Barabás O, Fuxreiter M, Simon I, Pichová I, Rumlová M, Zábranská H, Svergun D, Petoukhov M, Harmat V, Klement E, Hunyadi-Gulyás E, Medzihradszky KF, Kónya E, Vértessy BG. (2007)
Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins
Nucleic Acids Res. 35(2):495-505. doi:10.1093/nar/gkl1074
Europe PMC | doi


2006

Barabás O, Németh V, Vértessy BG. (2006)
Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62(Pt 4):399-401.
Europe PMC


2004

Barabás O, Pongrácz V, Kovári J, Wilmanns M, Vértessy BG. (2004)
Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase
J. Biol. Chem. 279(41):42907-42915. doi:10.1074/jbc.m406135200
Europe PMC | doi

Kovári J, Barabás O, Takács E, Békési A, Dubrovay Z, Pongrácz V, Zagyva I, Imre T, Szabó P, Vértessy BG. (2004)
Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme
J. Biol. Chem. 279(17):17932-17944. doi:10.1074/jbc.m313643200
Europe PMC | doi


2003

Barabás O, Rumlová M, Erdei A, Pongrácz V, Pichová I, Vértessy BG. (2003)
dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency
J. Biol. Chem. 278(40):38803-38812. doi:10.1074/jbc.m306967200
Europe PMC | doi


2002

Beke G, Gergely A, Szász G, Szentesi A, Nyitray J, Barabás O, Harmath V, Mátyus P. (2002)
Synthesis and stereochemistry of dispiro substituted pyridazines: application of ellipticity-absorbance ratio spectra for proving enantiomeric relationship by HPLC-CD/UV detection
Chirality 14(5):365-371. doi:10.1002/chir.10096
Europe PMC | doi


2001

Simo M, Csampai A, Harmat V, Barabas O, Magyarfalvy G. (2001)
Novel ring transformations of condensed 1,2,4 triazolo- 4,3-b pyridazine-6(5H)-one-3(2H)-thiones effected by dialkyl-acetylenedicarboxylates
Tetrahedron 57(33):7191-7198.
Europe PMC

Abran A, Csampai A, Kotschy A, Barabas O, Sohar P. (2001)
Study on ferrocenes, part 9 1 substrate selective transformations of some ferrocenylhydrazones
J Mol Struct 569(1-3):185-194.
Europe PMC

Elias O, Karolyhazy L, Stajer G, Fulop F, Czako K, Harmath V, Barabas O, Keseru K, Matyus P. (2001)
Theoretical and experimental studies on ring closure reactions of 4(5)-chloro-5(4)-hydroxyalkylamino-6-nitro-3 (2H)-pyridazinones
Theochem 545:75-96.
Europe PMC


2000

Barabas O, Menyhard DK, Bocskei Z, Simon K, Kiss-Ajzert I, Takacs K, Hermecz I. (2000)
Structural basis of solid solution formation during chiral resolution
Tetrahedron Asymmetry 11(20):4061-4070.
Europe PMC