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Staff at EMBL

Dr. John Briggs

Group Leader and Senior Scientist
Briggs Group Joint appointment: Cell Biology and Biophysics Unit

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

E-mail: send a mail


PhD 2004, Oxford University.
Postdoctoral research at the University of Munich.
Group Leader at EMBL since 2006.
Senior Scientist since 2013.
Group leader in the Molecular Medicine Partnership Unit.


The nucleocapsid domain of Gag is dispensable for actin incorporation into HIV-1 and for association of viral budding sites with cortical F-actin. Stauffer, S., Rahman, S.A., de Marco, A., Carlson, L.A., Glass, B., Oberwinkler, H., Herold, N., Briggs, J.A., Muller, B., Grunewald, K. & Krausslich, H.G. J Virol. 2014 Jul;88(14):7893-903. doi: 10.1128/JVI.00428-14. Epub 2014 Apr 30. Europe PMC

Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly. Bharat, T.A., Castillo Menendez, L.R., Hagen, W.J., Lux, V., Igonet, S., Schorb, M., Schur, F.K., Krausslich, H.G. & Briggs, J.A. Proc Natl Acad Sci U S A. 2014 May 19. pii: 201401455. Europe PMC

SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion. Bharat, T.A., Malsam, J., Hagen, W.J., Scheutzow, A., Sollner, T.H. & Briggs, J.A. EMBO Rep. 2014 Mar;15(3):308-14. doi: 10.1002/embr.201337807. Epub 2014 Feb 3. Europe PMC

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy. Nikic, I., Plass, T., Schraidt, O., Szymanski, J., Briggs, J.A., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2014;53(8):2245–2249. doi: 10.1002/anie.201309847. Europe PMC

Insights from reconstitution reactions of COPII vesicle formation using pure components and low mechanical perturbation. Daum, S., Kruger, D., Meister, A., Auerswald, J., Prinz, S., Briggs, J.A. & Bacia, K. Biol Chem. 2014 Jul 1;395(7-8):801-12. doi: 10.1515/hsz-2014-0117. Europe PMC

Determination of protein structure at 8.5A resolution using cryo-electron tomography and sub-tomogram averaging. Schur, F.K., Hagen, W.J., de Marco, A. & Briggs, J.A. J Struct Biol. 2013 Dec;184(3):394-400. doi: 10.1016/j.jsb.2013.10.015. Epub 2013Oct 30. Europe PMC

Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity. Schorb, M. & Briggs, J.A. Ultramicroscopy. 2013 Nov 11. pii: S0304-3991(13)00291-X. doi:10.1016/j.ultramic.2013.10.015. Europe PMC

The structure of the COPII transport-vesicle coat assembled on membranes. Zanetti, G., Prinz, S., Daum, S., Meister, A., Schekman, R., Bacia, K. & Briggs, J.A. Elife. 2013 Sep 17;2:e00951. doi: 10.7554/eLife.00951. Europe PMC

Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging. Szymborska, A., de Marco, A., Daigle, N., Cordes, V.C., Briggs, J.A. & Ellenberg, J. Science. 2013 Aug 9;341(6146):655-8. doi: 10.1126/science.1240672. Epub 2013 Jul11. Europe PMC

Variable Internal Flexibility Characterizes the Helical Capsid Formed by Agrobacterium VirE2 Protein on Single-Stranded DNA. Bharat, T.A., Zbaida, D., Eisenstein, M., Frankenstein, Z., Mehlman, T., Weiner, L., Sorzano, C.O., Barak, Y., Albeck, S., Briggs, J.A., Wolf, S.G. & Elbaum, M. Structure. 2013 Jul 2;21(7):1158-67. doi: 10.1016/j.str.2013.04.027. Epub 2013Jun 13. Europe PMC

Vesicle coats: structure, function, and general principles of assembly. Faini, M., Beck, R., Wieland, F.T. & Briggs, J.A. Trends Cell Biol. 2013 Jun;23(6):279-88. doi: 10.1016/j.tcb.2013.01.005. Epub2013 Feb 13. Europe PMC

Structural biology in situ--the potential of subtomogram averaging. Briggs, J.A. Curr Opin Struct Biol. 2013 Apr;23(2):261-7. doi: 10.1016/ Epub2013 Mar 4. Europe PMC

Tubular endocytosis drives remodelling of the apical surface during epithelial morphogenesis in Drosophila. Fabrowski, P., Necakov, A.S., Mumbauer, S., Loeser, E., Reversi, A., Streichan, S., Briggs, J.A. & De Renzis, S. Nat Commun. 2013;4:2244. doi: 10.1038/ncomms3244. Europe PMC

Role of the SP2 Domain and Its Proteolytic Cleavage in HIV-1 Structural Maturation and Infectivity. de Marco, A., Heuser, A.M., Glass, B., Krausslich, H.G., Muller, B. & Briggs, J.A. J Virol. 2012 Dec;86(24):13708-16. doi: 10.1128/JVI.01704-12. Epub 2012 Oct 10. Europe PMC

Plasma membrane reshaping during endocytosis is revealed by time-resolved electron tomography. Kukulski, W., Schorb, M., Kaksonen, M. & Briggs, J.A. Cell. 2012 Aug 3;150(3):508-20. doi: 10.1016/j.cell.2012.05.046. Europe PMC

Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2)-dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. Steringer, J.P., Bleicken, S., Andreas, H., Zacherl, S., Laussmann, M., Temmerman, K., Contreras, F.X., Bharat, T.A., Lechner, J., Muller, H.M., Briggs, J.A., Garcia-Saez, A.J. & Nickel, W. J Biol Chem. 2012 Aug 10;287(33):27659-69. Epub 2012 Jun 23. Europe PMC

Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy. Bharat, T.A., Davey, N.E., Ulbrich, P., Riches, J.D., de Marco, A., Rumlova, M., Sachse, C., Ruml, T. & Briggs, J.A. Nature. 2012 Jul 19;487(7407):385-9. Europe PMC

Complexin arrests a pool of docked vesicles for fast Ca(2+)-dependent release. Malsam, J., Parisotto, D., Bharat, T.A., Scheutzow, A., Krause, J.M., Briggs, J.A. & Sollner, T.H. EMBO J. 2012 Jun 15. doi: 10.1038/emboj.2012.164. Europe PMC

The structures of COPI-coated vesicles reveal alternate coatomer conformations and interactions. Faini, M., Prinz, S., Beck, R., Schorb, M., Riches, J.D., Bacia, K., Brugger, B., Wieland, F.T. & Briggs, J.A. Science. 2012 Jun 15;336(6087):1451-4. Epub 2012 May 24.
Link Europe PMC

Imaging cellular structure across scales with correlated light, superresolution, and electron microscopy. Briggs, J.; Lakadamyali Melike Molecular Biology of the cell, Volume: 23, Issue: 6, Pages: 979-980, DOI: 10.1091/mbc.E11-12-0971, Published: MAR 15 2012

Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. Bharat, T.A., Noda, T., Riches, J.D., Kraehling, V., Kolesnikova, L., Becker, S., Kawaoka, Y. & Briggs, J.A. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4275-80. doi:10.1073/pnas.1120453109. Epub 2012 Feb 27. Europe PMC

In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV. Hadravova, R., de Marco, A., Ulbrich, P., Stokrova, J., Dolezal, M., Pichova, I., Ruml, T., Briggs, J.A. & Rumlova, M. J Virol. 2012 Feb;86(3):1297-306. Epub 2011 Nov 16. Europe PMC

Precise, correlated fluorescence microscopy and electron tomography of lowicryl sections using fluorescent fiducial markers. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A. Methods Cell Biol. 2012;111:235-57. doi: 10.1016/B978-0-12-416026-2.00013-3. Europe PMC

Computational Identification of Novel Amino-Acid Interactions in HIV Gag via Correlated Evolution. Kalinina, O.V., Oberwinkler, H., Glass, B., Krausslich, H.G., Russell, R.B. & Briggs, J.A. PLoS One. 2012;7(8):e42468. Epub 2012 Aug 3. Europe PMC

Cryo-Electron Tomography of Marburg Virus Particles and Their Morphogenesis within Infected Cells. Bharat, T.A., Riches, J.D., Kolesnikova, L., Welsch, S., Krahling, V., Davey, N., Parsy, M.L., Becker, S. & Briggs, J.A. PLoS Biol. 2011 Nov;9(11):e1001196. Epub 2011 Nov 15. Europe PMC

Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. Beck, R., Prinz, S., Diestelkotter-Bachert, P., Rohling, S., Adolf, F., Hoehner, K., Welsch, S., Ronchi, P., Brugger, B., Briggs, J.A. & Wieland, F. J Cell Biol. 2011 Sep 5;194(5):765-77. Europe PMC

The molecular architecture of HIV. Briggs, J.A. & Krausslich, H.G. J Mol Biol. 2011 Jul 22;410(4):491-500. doi: 10.1016/j.jmb.2011.04.021. Europe PMC

Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A. J Cell Biol. 2011 Jan 10;192(1):111-9. doi: 10.1083/jcb.201009037. Epub 2011 Jan3. Europe PMC

Multibudded tubules formed by COPII on artificial liposomes. Bacia, K., Futai, E., Prinz, S., Meister, A., Daum, S., Glatte, D., Briggs, J.A. & Schekman, R. Sci Rep. 2011;1:17. doi: 10.1038/srep00017. Epub 2011 Jun 17. Europe PMC

Conserved and variable features of Gag structure and arrangement in immature retrovirus particles. de Marco, A., Davey, N.E., Ulbrich, P., Phillips, J.M., Lux, V., Riches, J.D., Fuzik, T., Ruml, T., Krausslich, H.G., Vogt, V.M. & Briggs, J.A. J Virol. 2010 Nov;84(22):11729-36. doi: 10.1128/JVI.01423-10. Epub 2010 Sep 1. Europe PMC

Structural Analysis of HIV-1 Maturation Using Cryo-Electron Tomography. de Marco, A., Muller, B., Glass, B., Riches, J.D., Krausslich, H.G. & Briggs, J.A. PLoS Pathog. 2010 Nov 24;6(11):e1001215. Europe PMC

Cryo Electron Tomography of Native HIV-1 Budding Sites. Carlson, L.A., de Marco, A., Oberwinkler, H., Habermann, A., Briggs, J.A., Krausslich, H.G. & Grunewald, K. PLoS Pathog. 2010 Nov 24;6(11):e1001173. Europe PMC

Electron tomography reveals the steps in filovirus budding. Welsch, S., Kolesnikova, L., Krahling, V., Riches, J.D., Becker, S. & Briggs, J.A. PLoS Pathog. 2010 Apr 29;6(4):e1000875. Europe PMC

Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. Martin, N., Welsch, S., Jolly, C., Briggs, J.A., Vaux, D. & Sattentau, Q.J. J Virol. 2010 Apr;84(7):3516-27. doi: 10.1128/JVI.02651-09. Epub 2010 Jan 20. Europe PMC

Contrast transfer function correction applied to cryo-electron tomography and sub-tomogram averaging. Zanetti, G., Riches, J.D., Fuller, S.D. & Briggs, J.A. J Struct Biol. 2009 Nov;168(2):305-12. doi: 10.1016/j.jsb.2009.08.002. Epub 2009Aug 8. Europe PMC

Computational model of membrane fission catalyzed by ESCRT-III. Fabrikant, G., Lata, S., Riches, J.D., Briggs, J.A., Weissenhorn, W. & Kozlov, M.M. PLoS Comput Biol. 2009 Nov;5(11):e1000575. Epub 2009 Nov 20. Europe PMC

Structure and assembly of immature HIV. Briggs, J.A., Riches, J.D., Glass, B., Bartonova, V., Zanetti, G. & Krausslich, H.G. Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11090-5. doi:10.1073/pnas.0903535106. Epub 2009 Jun 22. Europe PMC

Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis. Carlson, L.A., Briggs, J.A., Glass, B., Riches, J.D., Simon, M.N., Johnson, M.C., Muller, B., Grunewald, K. & Krausslich, H.G. Cell Host Microbe. 2008 Dec 11;4(6):592-9. Europe PMC

HIV-1-cellular interactions analyzed by single virus tracing. Endress, T., Lampe, M., Briggs, J.A., Krausslich, H.G., Brauchle, C., Muller, B. & Lamb, D.C. Eur Biophys J. 2008 Oct;37(8):1291-301. Epub 2008 Apr 10. Europe PMC

Structure of a hexameric RNA packaging motor in a viral polymerase complex. Huiskonen, J.T., Jaalinoja, H.T., Briggs, J.A., Fuller, S.D. & Butcher, S.J. J Struct Biol. 2007 May;158(2):156-64. Epub 2006 Oct 7. Europe PMC

Double-labelled HIV-1 particles for study of virus-cell interaction. Lampe, M., Briggs, J.A., Endress, T., Glass, B., Riegelsberger, S., Krausslich, H.G., Lamb, D.C., Brauchle, C. & Muller, B. Virology. 2007 Mar 30;360(1):92-104. Epub 2006 Nov 9. Europe PMC

Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ. Zanetti, G., Briggs, J.A., Grunewald, K., Sattentau, Q.J. & Fuller, S.D. PLoS Pathog. 2006 Aug;2(8):e83. Europe PMC

Cryo-electron microscopy reveals conserved and divergent features of gag packing in immature particles of Rous sarcoma virus and human immunodeficiency virus. Briggs, J.A., Johnson, M.C., Simon, M.N., Fuller, S.D. & Vogt, V.M. J Mol Biol. 2006 Jan 6;355(1):157-68. Epub 2005 Nov 2. Europe PMC

The mechanism of HIV-1 core assembly: insights from three-dimensional reconstructions of authentic virions. Briggs, J.A., Grunewald, K., Glass, B., Forster, F., Krausslich, H.G. & Fuller, S.D. Structure. 2006 Jan;14(1):15-20. Europe PMC

Classification and three-dimensional reconstruction of unevenly distributed or symmetry mismatched features of icosahedral particles. Briggs, J.A., Huiskonen, J.T., Fernando, K.V., Gilbert, R.J., Scotti, P., Butcher, S.J. & Fuller, S.D. J Struct Biol. 2005 Jun;150(3):332-9. Epub 2005 Apr 19. Europe PMC

The stoichiometry of Gag protein in HIV-1. Briggs, J.A., Simon, M.N., Gross, I., Krausslich, H.G., Fuller, S.D., Vogt, V.M. & Johnson, M.C. Nat Struct Mol Biol. 2004 Jul;11(7):672-5. Epub 2004 Jun 20. Europe PMC

Cryoelectron microscopy of mouse mammary tumor virus. Briggs, J.A., Watson, B.E., Gowen, B.E. & Fuller, S.D. J Virol. 2004 Mar;78(5):2606-8. Europe PMC

Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper. Schwarz-Linek, U., Werner, J.M., Pickford, A.R., Gurusiddappa, S., Kim, J.H., Pilka, E.S., Briggs, J.A., Gough, T.S., Hook, M., Campbell, I.D. & Potts, J.R. Nature. 2003 May 8;423(6936):177-81. Europe PMC

Structural organization of authentic, mature HIV-1 virions and cores. Briggs, J.A., Wilk, T., Welker, R., Krausslich, H.G. & Fuller, S.D. EMBO J. 2003 Apr 1;22(7):1707-15. Europe PMC

Do lipid rafts mediate virus assembly and pseudotyping? Briggs, J.A., Wilk, T. & Fuller, S.D. J Gen Virol. 2003 Apr;84(Pt 4):757-68. Europe PMC

Contribution of energy values to the analysis of global searching molecular dynamics simulations of transmembrane helical bundles. Torres, J., Briggs, J.A. & Arkin, I.T. Biophys J. 2002 Jun;82(6):3063-71. Europe PMC

Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes. Rous, B.A., Reaves, B.J., Ihrke, G., Briggs, J.A., Gray, S.R., Stephens, D.J., Banting, G. & Luzio, J.P. Mol Biol Cell. 2002 Mar;13(3):1071-82. Europe PMC

Convergence of experimental, computational and evolutionary approaches predicts the presence of a tetrameric form for CD3-zeta. Torres, J., Briggs, J.A. & Arkin, I.T. J Mol Biol. 2002 Feb 15;316(2):375-84. Europe PMC

Multiple site-specific infrared dichroism of CD3-zeta, a transmembrane helix bundle. Torres, J., Briggs, J.A. & Arkin, I.T. J Mol Biol. 2002 Feb 15;316(2):365-74. Europe PMC

A new method to model membrane protein structure based on silent amino acid substitutions. Briggs, J.A., Torres, J. & Arkin, I.T. Proteins. 2001 Aug 15;44(3):370-5. Europe PMC