Staff at EMBL

Dr. John Briggs

Group Leader
Briggs Group Joint appointment: Cell Biology and Biophysics Unit

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Biography

PhD 2004, Oxford University.
Postdoctoral research at the University of Munich.
Group Leader at EMBL since 2006.

Publications

Role of the SP2 Domain and Its Proteolytic Cleavage in HIV-1 Structural Maturation and Infectivity. de Marco, A., Heuser, A.M., Glass, B., Krausslich, H.G., Muller, B. & Briggs, J.A. J Virol. 2012 Dec;86(24):13708-16. doi: 10.1128/JVI.01704-12. Epub 2012 Oct 10. PubMed

Plasma Membrane Reshaping during Endocytosis Is Revealed by Time-Resolved Electron Tomography. Kukulski, W., Schorb, M., Kaksonen, M. & Briggs, J.A. Cell. 2012 Aug 3;150(3):508-20. PubMed

Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2)-dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. Steringer, J.P., Bleicken, S., Andreas, H., Zacherl, S., Laussmann, M., Temmerman, K., Contreras, F.X., Bharat, T.A., Lechner, J., Muller, H.M., Briggs, J.A., Garcia-Saez, A.J. & Nickel, W. J Biol Chem. 2012 Aug 10;287(33):27659-69. Epub 2012 Jun 23. PubMed

Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy. Bharat, T.A., Davey, N.E., Ulbrich, P., Riches, J.D., de Marco, A., Rumlova, M., Sachse, C., Ruml, T. & Briggs, J.A. Nature. 2012 Jul 19;487(7407):385-9. PubMed

Complexin arrests a pool of docked vesicles for fast Ca(2+)-dependent release. Malsam, J., Parisotto, D., Bharat, T.A., Scheutzow, A., Krause, J.M., Briggs, J.A. & Sollner, T.H. EMBO J. 2012 Jun 15. doi: 10.1038/emboj.2012.164. PubMed

The structures of COPI-coated vesicles reveal alternate coatomer conformations and interactions. Faini, M., Prinz, S., Beck, R., Schorb, M., Riches, J.D., Bacia, K., Brugger, B., Wieland, F.T. & Briggs, J.A. Science. 2012 Jun 15;336(6087):1451-4. Epub 2012 May 24.
Link PubMed

Imaging cellular structure across scales with correlated light, superresolution, and electron microscopy. Briggs, J.; Lakadamyali Melike Molecular Biology of the cell, Volume: 23, Issue: 6, Pages: 979-980, DOI: 10.1091/mbc.E11-12-0971, Published: MAR 15 2012

Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. Bharat, T.A., Noda, T., Riches, J.D., Kraehling, V., Kolesnikova, L., Becker, S., Kawaoka, Y. & Briggs, J.A. Proc Natl Acad Sci U S A. 2012 Feb 27. PubMed

In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV. Hadravova, R., de Marco, A., Ulbrich, P., Stokrova, J., Dolezal, M., Pichova, I., Ruml, T., Briggs, J.A. & Rumlova, M. J Virol. 2012 Feb;86(3):1297-306. Epub 2011 Nov 16. PubMed

Precise, correlated fluorescence microscopy and electron tomography of lowicryl sections using fluorescent fiducial markers. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A. Methods Cell Biol. 2012;111:235-57. PubMed

Computational Identification of Novel Amino-Acid Interactions in HIV Gag via Correlated Evolution. Kalinina, O.V., Oberwinkler, H., Glass, B., Krausslich, H.G., Russell, R.B. & Briggs, J.A. PLoS One. 2012;7(8):e42468. Epub 2012 Aug 3. PubMed

Cryo-Electron Tomography of Marburg Virus Particles and Their Morphogenesis within Infected Cells. Bharat, T.A., Riches, J.D., Kolesnikova, L., Welsch, S., Krahling, V., Davey, N., Parsy, M.L., Becker, S. & Briggs, J.A. PLoS Biol. 2011 Nov;9(11):e1001196. Epub 2011 Nov 15. PubMed

Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. Beck, R., Prinz, S., Diestelkotter-Bachert, P., Rohling, S., Adolf, F., Hoehner, K., Welsch, S., Ronchi, P., Brugger, B., Briggs, J.A. & Wieland, F. J Cell Biol. 2011 Sep 5;194(5):765-77. PubMed

The Molecular Architecture of HIV. Briggs, J.A. & Krausslich, H.G. J Mol Biol. 2011 Jul 22;410(4):491-500. PubMed

Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A. J Cell Biol. 2011 Jan 10;192(1):111-9. doi: 10.1083/jcb.201009037. Epub 2011 Jan3. PubMed

Multibudded tubules formed by COPII on artificial liposomes. Bacia, K., Futai, E., Prinz, S., Meister, A., Daum, S., Glatte, D., Briggs, J.A. & Schekman, R. Sci Rep. 2011;1:17. Epub 2011 Jun 17. PubMed

Structural Analysis of HIV-1 Maturation Using Cryo-Electron Tomography. de Marco, A., Muller, B., Glass, B., Riches, J.D., Krausslich, H.G. & Briggs, J.A. PLoS Pathog. 2010 Nov 24;6(11):e1001215. PubMed

Cryo Electron Tomography of Native HIV-1 Budding Sites. Carlson, L.A., de Marco, A., Oberwinkler, H., Habermann, A., Briggs, J.A., Krausslich, H.G. & Grunewald, K. PLoS Pathog. 2010 Nov 24;6(11):e1001173. PubMed

Conserved and variable features of Gag structure and arrangement in immature retrovirus particles. de Marco, A., Davey, N.E., Ulbrich, P., Phillips, J.M., Lux, V., Riches, J.D., Fuzik, T., Ruml, T., Krausslich, H.G., Vogt, V.M. & Briggs, J.A. J Virol. 2010 Sep 1. PubMed

Electron tomography reveals the steps in filovirus budding. Welsch, S., Kolesnikova, L., Krahling, V., Riches, J.D., Becker, S. & Briggs, J.A. PLoS Pathog. 2010 Apr 29;6(4):e1000875. PubMed

Virological synapse-mediated spread of Human Immunodeficiency Virus type-1 between T cells is sensitive to entry inhibition. Martin, N., Welsch, S., Jolly, C., Briggs, J.A., Vaux, D. & Sattentau, Q.J. J Virol. 2010 Jan 20. PubMed

Computational model of membrane fission catalyzed by ESCRT-III. Fabrikant, G., Lata, S., Riches, J.D., Briggs, J.A., Weissenhorn, W. & Kozlov, M.M. PLoS Comput Biol. 2009 Nov;5(11):e1000575. Epub 2009 Nov 20. PubMed

Contrast transfer function correction applied to cryo-electron tomography and sub-tomogram averaging. Zanetti, G., Riches, J.D., Fuller, S.D. & Briggs, J.A. J Struct Biol. 2009 Aug 7. PubMed

Structure and assembly of immature HIV. Briggs, J.A., Riches, J.D., Glass, B., Bartonova, V., Zanetti, G. & Krausslich, H.G. Proc Natl Acad Sci U S A. 2009 Jun 22. PubMed

Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis. Carlson, L.A., Briggs, J.A., Glass, B., Riches, J.D., Simon, M.N., Johnson, M.C., Muller, B., Grunewald, K. & Krausslich, H.G. Cell Host Microbe. 2008 Dec 11;4(6):592-9. PubMed

HIV-1-cellular interactions analyzed by single virus tracing. Endress, T., Lampe, M., Briggs, J.A., Krausslich, H.G., Brauchle, C., Muller, B. & Lamb, D.C. Eur Biophys J. 2008 Oct;37(8):1291-301. Epub 2008 Apr 10. PubMed

Structure of a hexameric RNA packaging motor in a viral polymerase complex. Huiskonen, J.T., Jaalinoja, H.T., Briggs, J.A., Fuller, S.D. & Butcher, S.J. J Struct Biol. 2007 May;158(2):156-64. Epub 2006 Oct 7. PubMed

Double-labelled HIV-1 particles for study of virus-cell interaction. Lampe, M., Briggs, J.A., Endress, T., Glass, B., Riegelsberger, S., Krausslich, H.G., Lamb, D.C., Brauchle, C. & Muller, B. Virology. 2007 Mar 30;360(1):92-104. Epub 2006 Nov 9. PubMed

Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ. Zanetti, G., Briggs, J.A., Grunewald, K., Sattentau, Q.J. & Fuller, S.D. PLoS Pathog. 2006 Aug;2(8):e83. PubMed

Cryo-electron microscopy reveals conserved and divergent features of gag packing in immature particles of Rous sarcoma virus and human immunodeficiency virus. Briggs, J.A., Johnson, M.C., Simon, M.N., Fuller, S.D. & Vogt, V.M. J Mol Biol. 2006 Jan 6;355(1):157-68. Epub 2005 Nov 2. PubMed

The mechanism of HIV-1 core assembly: insights from three-dimensional reconstructions of authentic virions. Briggs, J.A., Grunewald, K., Glass, B., Forster, F., Krausslich, H.G. & Fuller, S.D. Structure. 2006 Jan;14(1):15-20. PubMed

Classification and three-dimensional reconstruction of unevenly distributed or symmetry mismatched features of icosahedral particles. Briggs, J.A., Huiskonen, J.T., Fernando, K.V., Gilbert, R.J., Scotti, P., Butcher, S.J. & Fuller, S.D. J Struct Biol. 2005 Jun;150(3):332-9. Epub 2005 Apr 19. PubMed

The stoichiometry of Gag protein in HIV-1. Briggs, J.A., Simon, M.N., Gross, I., Krausslich, H.G., Fuller, S.D., Vogt, V.M. & Johnson, M.C. Nat Struct Mol Biol. 2004 Jul;11(7):672-5. Epub 2004 Jun 20. PubMed

Cryoelectron microscopy of mouse mammary tumor virus. Briggs, J.A., Watson, B.E., Gowen, B.E. & Fuller, S.D. J Virol. 2004 Mar;78(5):2606-8. PubMed

Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper. Schwarz-Linek, U., Werner, J.M., Pickford, A.R., Gurusiddappa, S., Kim, J.H., Pilka, E.S., Briggs, J.A., Gough, T.S., Hook, M., Campbell, I.D. & Potts, J.R. Nature. 2003 May 8;423(6936):177-81. PubMed

Structural organization of authentic, mature HIV-1 virions and cores. Briggs, J.A., Wilk, T., Welker, R., Krausslich, H.G. & Fuller, S.D. EMBO J. 2003 Apr 1;22(7):1707-15. PubMed

Do lipid rafts mediate virus assembly and pseudotyping? Briggs, J.A., Wilk, T. & Fuller, S.D. J Gen Virol. 2003 Apr;84(Pt 4):757-68. PubMed

Contribution of energy values to the analysis of global searching molecular dynamics simulations of transmembrane helical bundles. Torres, J., Briggs, J.A. & Arkin, I.T. Biophys J. 2002 Jun;82(6):3063-71. PubMed

Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes. Rous, B.A., Reaves, B.J., Ihrke, G., Briggs, J.A., Gray, S.R., Stephens, D.J., Banting, G. & Luzio, J.P. Mol Biol Cell. 2002 Mar;13(3):1071-82. PubMed

Multiple site-specific infrared dichroism of CD3-zeta, a transmembrane helix bundle. Torres, J., Briggs, J.A. & Arkin, I.T. J Mol Biol. 2002 Feb 15;316(2):365-74. PubMed

Convergence of experimental, computational and evolutionary approaches predicts the presence of a tetrameric form for CD3-zeta. Torres, J., Briggs, J.A. & Arkin, I.T. J Mol Biol. 2002 Feb 15;316(2):375-84. PubMed

A new method to model membrane protein structure based on silent amino acid substitutions. Briggs, J.A., Torres, J. & Arkin, I.T. Proteins. 2001 Aug 15;44(3):370-5. PubMed