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Staff at EMBL

Dr. Edward Lemke

Group Leader
Lemke Group Joint appointment: Cell Biology and Biophysics Unit

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Tel: +49 6221 387-8536
E-mail: send a mail

Biography

PhD, Max Planck Institute for Biophysical Chemistry, Göttingen.
Research Associate, the Scripps Research Institute, USA.
Group leader at EMBL since 2009.
Joint appointment with Cell Biology and Biophysics Unit.
Emmy Noether group leader since 2010.

Publications

The Exploding Genetic Code. Lemke, E.A. Chembiochem. 2014 Jul 30. doi: 10.1002/cbic.201402362. Europe PMC

The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor. Dunne, M., Mertens, H.D., Garefalaki, V., Jeffries, C.M., Thompson, A., Lemke, E.A., Svergun, D.I., Mayer, M.J., Narbad, A. & Meijers, R. PLoS Pathog. 2014 Jul 24;10(7):e1004228. doi: 10.1371/journal.ppat.1004228.eCollection 2014 Jul. Europe PMC

Mapping Multivalency and Differential Affinities within Large Intrinsically Disordered Protein Complexes with Segmental Motion Analysis. Milles, S. & Lemke, E.A. Angew Chem Int Ed Engl. 2014 Jun 4. doi: 10.1002/anie.201403694. Europe PMC

Continuous throughput and long-term observation of single-molecule FRET without immobilization. Tyagi, S., VanDelinder, V., Banterle, N., Fuertes, G., Milles, S., Agez, M. & Lemke, E.A. Nat Methods. 2014 Mar;11(3):297-300. doi: 10.1038/nmeth.2809. Epub 2014 Jan 19. Europe PMC

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy. Nikic, I., Plass, T., Schraidt, O., Szymanski, J., Briggs, J.A., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2014;53(8):2245–2249. doi: 10.1002/anie.201309847. Europe PMC

Mapping multivalency and differential affinities within large intrinsically disordered protein complexes with segmental motion analysis. Milles, S. & Lemke, E.A. Angew Chem Int Ed Engl. 2014, in press. doi: 10.1002/anie.201403694.

Fourier ring correlation as a resolution criterion for super-resolution microscopy. Banterle, N., Bui, K.H., Lemke, E.A. & Beck, M. J Struct Biol. 2013 Sep;183(3):363-7. doi: 10.1016/j.jsb.2013.05.004. Epub 2013May 16. Europe PMC

A new family of bioorthogonally applicable fluorogenic labels. Herner, A., Nikic, I., Kallay, M., Lemke, E.A. & Kele, P. Org Biomol Chem. 2013 May 28;11(20):3297-306. doi: 10.1039/c3ob40296g. Europe PMC

Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines. Ori, A., Banterle, N., Iskar, M., Andres-Pons, A., Escher, C., Khanh Bui, H., Sparks, L., Solis-Mezarino, V., Rinner, O., Bork, P., Lemke, E.A. & Beck, M. Mol Syst Biol. 2013 Mar 19;9:648. doi: 10.1038/msb.2013.4. Europe PMC

Facilitated aggregation of FG nucleoporins under molecular crowding conditions. Milles, S., Huy Bui, K., Koehler, C., Eltsov, M., Beck, M. & Lemke, E.A. EMBO Rep. 2013 Feb;14(2):178-83. doi: 10.1038/embor.2012.204. Epub 2012 Dec 14. Europe PMC

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Lukinavicius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Correa IR, J.r, Luo, Z.G., Schultz, C., Lemke, E.A., Heppenstall, P., Eggeling, C., Manley, S. & Johnsson, K. Nat Chem. 2013 Feb;5(2):132-9. doi: 10.1038/nchem.1546. Epub 2013 Jan 6. Europe PMC

What precision-protein-tuning and nano-resolved single molecule sciences can do for each other. Milles, S. & Lemke, E.A. Bioessays. 2013 Jan;35(1):65-74. doi: 10.1002/bies.201200094. Epub 2012 Nov 13. Europe PMC

Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins. Tyagi, S. & Lemke, E.A. Methods Cell Biol. 2013;113:169-87. doi: 10.1016/B978-0-12-407239-8.00009-4. Europe PMC

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range. Milles, S., Koehler, C., Gambin, Y., Deniz, A.A. & Lemke, E.A. Mol Biosyst. 2012 Oct;8(10):2531-4. doi: 10.1039/c2mb25135c. Europe PMC

Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation. Borrmann, A., Milles, S., Plass, T., Dommerholt, J., Verkade, J.M., Wiessler, M., Schultz, C., van Hest, J.C., van Delft, F.L. & Lemke, E.A. Chembiochem. 2012 Sep 24;13(14):2094-9. doi: 10.1002/cbic.201200407. Epub 2012Sep 3. Europe PMC

Conserved features of intermediates in amyloid assembly determine their benign or toxic states. Krishnan, R., Goodman, J.L., Mukhopadhyay, S., Pacheco, C.D., Lemke, E.A., Deniz, A.A. & Lindquist, S. Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11172-7. doi:10.1073/pnas.1209527109. Epub 2012 Jun 27. Europe PMC

Amino acids for diels-alder reactions in living cells. Plass, T., Milles, S., Koehler, C., Szymanski, J., Mueller, R., Wiessler, M., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2012 Apr 23;51(17):4166-70. doi: 10.1002/anie.201108231.Epub 2012 Mar 30. Europe PMC

Click strategies for single-molecule protein fluorescence. Milles, S., Tyagi, S., Banterle, N., Koehler, C., Vandelinder, V., Plass, T., Neal, A.P. & Lemke, E.A. J Am Chem Soc. 2012 Mar 21;134(11):5187-95. Epub 2012 Mar 5. Europe PMC

Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153. Milles, S. & Lemke, E.A. Biophys J. 2011 Oct 5;101(7):1710-9. doi: 10.1016/j.bpj.2011.08.025. Europe PMC

Principles for designing fluorescent sensors and reporters. Lemke, E.A. & Schultz, C. Nat Chem Biol. 2011 Jul 18;7(8):480-3. doi: 10.1038/nchembio.620. Europe PMC

Genetically encoded copper-free click chemistry. Plass, T., Milles, S., Koehler, C., Schultz, C. & Lemke, E.A. Angew Chem Int Ed Engl. 2011 Apr 18;50(17):3878-81. doi:10.1002/anie.201008178. Epub 2011 Mar 23. Europe PMC

Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing. Gambin, Y., VanDelinder, V., Ferreon, A.C., Lemke, E.A., Groisman, A. & Deniz, A.A. Nat Methods. 2011 Mar;8(3):239-41. doi: 10.1038/nmeth.1568. Epub 2011 Feb 6. Europe PMC

2.05 - Förster Resonance Energy Transfer. Lemke, E.A. & Deniz, A.A. In Comprehensive Nanoscience and Technology, edited by David L. Andrews, Gregory D. Scholes and Gary P. Wiederrecht, Academic Press, Amsterdam, 2011, Pages 127-151

Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities. Lemke, E.A. Methods Mol Biol. 2011;751:3-15. doi: 10.1007/978-1-61779-151-2_1. Europe PMC

Precision control of cellular pathways with light. Lemke, E.A. Chembiochem. 2010 Sep 3;11(13):1825-7. Europe PMC

Microfluidic device for single-molecule experiments with enhanced photostability. Lemke, E.A., Gambin, Y., Vandelinder, V., Brustad, E.M., Liu, H.W., Schultz, P.G., Groisman, A. & Deniz, A.A. J Am Chem Soc. 2009 Sep 30;131(38):13610-2. Europe PMC

Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae. Lee, H.S., Guo, J., Lemke, E.A., Dimla, R.D. & Schultz, P.G. J Am Chem Soc. 2009 Sep 16;131(36):12921-3. Europe PMC

Direct single-molecule observation of a protein living in two opposed native structures. Gambin, Y., Schug, A., Lemke, E.A., Lavinder, J.J., Ferreon, A.C., Magliery, T.J., Onuchic, J.N. & Deniz, A.A. Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10153-8. doi:10.1073/pnas.0904461106. Epub 2009 Jun 8. Europe PMC

Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence. Ferreon, A.C., Gambin, Y., Lemke, E.A. & Deniz, A.A. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5645-50. Epub 2009 Mar 17. Europe PMC

A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer. Brustad, E.M., Lemke, E.A., Schultz, P.G. & Deniz, A.A. J Am Chem Soc. 2008 Dec 31;130(52):17664-5. Europe PMC

Single-molecule biophysics: at the interface of biology, physics and chemistry. Deniz, A.A., Mukhopadhyay, S. & Lemke, E.A. J R Soc Interface. 2008 Jan 6;5(18):15-45. Europe PMC

Control of protein phosphorylation with a genetically encoded photocaged amino acid. Lemke, E.A., Summerer, D., Geierstanger, B.H., Brittain, S.M. & Schultz, P.G. Nat Chem Biol. 2007 Dec;3(12):769-72. Epub 2007 Oct 28. Europe PMC

Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients. Henkel, A.W., Dittrich, P.S., Groemer, T.W., Lemke, E.A., Klingauf, J., Klafki, H.W., Lewczuk, P., Esselmann, H., Schwille, P., Kornhuber, J. & Wiltfang, J. Mol Psychiatry. 2007 Jun;12(6):601-10. Epub 2007 Feb 6. Europe PMC

A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures. Mukhopadhyay, S., Krishnan, R., Lemke, E.A., Lindquist, S. & Deniz, A.A. Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2649-54. Epub 2007 Feb 13. Europe PMC

Molecular anatomy of a trafficking organelle. Takamori, S., Holt, M., Stenius, K., Lemke, E.A., Gronborg, M., Riedel, D., Urlaub, H., Schenck, S., Brugger, B., Ringler, P., Muller, S.A., Rammner, B., Grater, F., Hub, J.S., De Groot, B.L., Mieskes, G., Moriyama, Y., Klingauf, J., Grubmuller, H., Heuser, J., Wieland, F. & Jahn, R. Cell. 2006 Nov 17;127(4):831-46. Europe PMC

Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity. Lemke, E.A. & Klingauf, J. J Neurosci. 2005 Nov 23;25(47):11034-44. Europe PMC

Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy. Jordan, R., Lemke, E.A. & Klingauf, J. Biophys J. 2005 Sep;89(3):2091-102. Epub 2005 Jun 24. Europe PMC

Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli. Chakraborty, R., Lemke, E.A., Cao, Z., Klebba, P.E. & van der Helm, D. Biometals. 2003 Dec;16(4):507-18. Europe PMC