Lemke GroupPublications
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
Lukinavicius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Correa IR, J.r, Luo, Z.G., Schultz, C., Lemke, E.A., Heppenstall, P., Eggeling, C., Manley, S. & Johnsson, K.
Nat Chem. 2013 Feb;5(2):132-9. doi: 10.1038/nchem.1546. Epub 2013 Jan 6. PubMed
What precision-protein-tuning and nano-resolved single molecule sciences can do for each other.
Milles, S. & Lemke, E.A.
Bioessays. 2013 Jan;35(1):65-74. doi: 10.1002/bies.201200094. Epub 2012 Nov 13. PubMed
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Ori, A., Banterle, N., Iskar, M., Andres-Pons, A., Escher, C., Khanh Bui, H., Sparks, L., Solis-Mezarino, V., Rinner, O., Bork, P., Lemke, E.A. & Beck, M.
Mol Syst Biol. 2013 Mar 19;9:648. doi: 10.1038/msb.2013.4. PubMed
Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins.
Tyagi, S. & Lemke, E.A.
Methods Cell Biol. 2013;113:169-87. doi: 10.1016/B978-0-12-407239-8.00009-4. PubMed
Facilitated aggregation of FG nucleoporins under molecular crowding conditions.
Milles, S., Huy Bui, K., Koehler, C., Eltsov, M., Beck, M. & Lemke, E.A.
EMBO Rep. 2012 Dec 14. doi: 10.1038/embor.2012.204. PubMed
Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation.
Borrmann, A., Milles, S., Plass, T., Dommerholt, J., Verkade, J.M., Wiessler, M., Schultz, C., van Hest, J.C., van Delft, F.L. & Lemke, E.A.
Chembiochem. 2012 Sep 24;13(14):2094-9. doi: 10.1002/cbic.201200407. Epub 2012Sep 3. PubMed
Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.
Milles, S., Koehler, C., Gambin, Y., Deniz, A.A. & Lemke, E.A.
Mol Biosyst. 2012 Jun 27. PubMed
Conserved features of intermediates in amyloid assembly determine their benign or toxic states.
Krishnan, R., Goodman, J.L., Mukhopadhyay, S., Pacheco, C.D., Lemke, E.A., Deniz, A.A. & Lindquist, S.
Proc Natl Acad Sci U S A. 2012 Jun 27. PubMed
Amino acids for diels-alder reactions in living cells.
Plass, T., Milles, S., Koehler, C., Szymanski, J., Mueller, R., Wiessler, M., Schultz, C. & Lemke, E.A.
Angew Chem Int Ed Engl. 2012 Apr 23;51(17):4166-70. doi: 10.1002/anie.201108231.Epub 2012 Mar 30. PubMed
Click strategies for single-molecule protein fluorescence.
Milles, S., Tyagi, S., Banterle, N., Koehler, C., Vandelinder, V., Plass, T., Neal, A.P. & Lemke, E.A.
J Am Chem Soc. 2012 Mar 21;134(11):5187-95. Epub 2012 Mar 5. PubMed
Single Molecule Study of the Intrinsically Disordered FG-Repeat Nucleoporin 153.
Milles, S. & Lemke, E.A.
Biophys J. 2011 Oct 5;101(7):1710-9. PubMed
Principles for designing fluorescent sensors and reporters.
Lemke, E.A. & Schultz, C.
Nat Chem Biol. 2011 Jul 18;7(8):480-3. doi: 10.1038/nchembio.620. PubMed
Genetically encoded copper-free click chemistry.
Plass, T., Milles, S., Koehler, C., Schultz, C. & Lemke, E.A.
Angew Chem Int Ed Engl. 2011 Apr 18;50(17):3878-81. doi:10.1002/anie.201008178. Epub 2011 Mar 23. PubMed
Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing.
Gambin, Y., Vandelinder, V., Ferreon, A.C., Lemke, E.A., Groisman, A. & Deniz, A.A.
Nat Methods. 2011 Feb 6. PubMed
Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities.
Lemke, E.A.
Methods Mol Biol. 2011;751:3-15. PubMed
Precision control of cellular pathways with light.
Lemke, E.A.
Chembiochem. 2010 Sep 3;11(13):1825-7. PubMed
Forster Resonance Energy Transfer.
Lemke, E.A., Deniz, A.A.,
Encyclopedia of Comprehensive Nanoscience and Technology (2010, 2.04, 127-151)
Microfluidic device for single-molecule experiments with enhanced photostability.
Lemke, E.A., Gambin, Y., Vandelinder, V., Brustad, E.M., Liu, H.W., Schultz, P.G., Groisman, A. & Deniz, A.A.
J Am Chem Soc. 2009 Sep 30;131(38):13610-2. PubMed
Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae.
Lee, H.S., Guo, J., Lemke, E.A., Dimla, R.D. & Schultz, P.G.
J Am Chem Soc. 2009 Sep 16;131(36):12921-3. PubMed
Direct single-molecule observation of a protein living in two opposed native structures.
Gambin, Y., Schug, A., Lemke, E.A., Lavinder, J.J., Ferreon, A.C., Magliery, T.J., Onuchic, J.N. & Deniz, A.A.
Proc Natl Acad Sci U S A. 2009 Jun 8. PubMed
Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence.
Ferreon, A.C., Gambin, Y., Lemke, E.A. & Deniz, A.A.
Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5645-50. Epub 2009 Mar 17. PubMed
A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer.
Brustad, E.M., Lemke, E.A., Schultz, P.G. & Deniz, A.A.
J Am Chem Soc. 2008 Dec 31;130(52):17664-5. PubMed
Single-molecule biophysics: at the interface of biology, physics and chemistry.
Deniz, A.A., Mukhopadhyay, S. & Lemke, E.A.
J R Soc Interface. 2008 Jan 6;5(18):15-45. PubMed
Control of protein phosphorylation with a genetically encoded photocaged amino acid.
Lemke, E.A., Summerer, D., Geierstanger, B.H., Brittain, S.M. & Schultz, P.G.
Nat Chem Biol. 2007 Dec;3(12):769-72. Epub 2007 Oct 28. PubMed
Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients.
Henkel, A.W., Dittrich, P.S., Groemer, T.W., Lemke, E.A., Klingauf, J., Klafki, H.W., Lewczuk, P., Esselmann, H., Schwille, P., Kornhuber, J. & Wiltfang, J.
Mol Psychiatry. 2007 Jun;12(6):601-10. Epub 2007 Feb 6. PubMed
A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures.
Mukhopadhyay, S., Krishnan, R., Lemke, E.A., Lindquist, S. & Deniz, A.A.
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2649-54. Epub 2007 Feb 13. PubMed
Molecular anatomy of a trafficking organelle.
Takamori, S., Holt, M., Stenius, K., Lemke, E.A., Gronborg, M., Riedel, D., Urlaub, H., Schenck, S., Brugger, B., Ringler, P., Muller, S.A., Rammner, B., Grater, F., Hub, J.S., De Groot, B.L., Mieskes, G., Moriyama, Y., Klingauf, J., Grubmuller, H., Heuser, J., Wieland, F. & Jahn, R.
Cell. 2006 Nov 17;127(4):831-46. PubMed
Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.
Lemke, E.A. & Klingauf, J.
J Neurosci. 2005 Nov 23;25(47):11034-44. PubMed
Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy.
Jordan, R., Lemke, E.A. & Klingauf, J.
Biophys J. 2005 Sep;89(3):2091-102. Epub 2005 Jun 24. PubMed
Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli.
Chakraborty, R., Lemke, E.A., Cao, Z., Klebba, P.E. & van der Helm, D.
Biometals. 2003 Dec;16(4):507-18. PubMed
What precision-protein-tuning and nano-resolved single molecule sciences can do for each other.
Milles S, Lemke EA.
Bioessays