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Lemke GroupPublications

Total: 39 publication(s)

2014

Lemke EA. (2014)
Special Issue: Expanding the Genetic Code
CHEMBIOCHEM 15(12):1691-1694. doi:10.1002/cbic.201402362
Europe PMC | doi

Lemke EA. (2014)
The Exploding Genetic Code
Chembiochem 15(12):1691-1694. doi:10.1002/cbic.201402362
Europe PMC | doi

Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R. (2014)
The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor
PLoS Pathog. 10(7):e1004228. doi:10.1371/journal.ppat.1004228
Europe PMC | doi

Milles S, Lemke EA. (2014)
Mapping Multivalency and Differential Affinities within Large Intrinsically Disordered Protein Complexes with Segmental Motion Analysis.
Angew. Chem. Int. Ed. Engl. 53(28):7364-7367. doi:10.1002/anie.201403694
Europe PMC | doi

Herner A, Estrada Girona G, Nikic I, Kallay M, Lemke EA, Kele P. (2014)
New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large Stokes-shifts.
Bioconjug. Chem. doi:10.1021/bc500235p
Europe PMC | doi

Tyagi S, Vandelinder V, Banterle N, Fuertes G, Milles S, Agez M, Lemke EA. (2014)
Continuous throughput and long-term observation of single-molecule FRET without immobilization
Nat. Methods 11(3):297-300. doi:10.1038/nmeth.2809
Europe PMC | doi

Nikić I, Plass T, Schraidt O, Szymański J, Briggs JA, Schultz C, Lemke EA. (2014)
Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy
Angew. Chem. Int. Ed. Engl. 53(8):2245-2249. doi:10.1002/anie.201309847
Europe PMC | doi


2013

Banterle N, Bui KH, Lemke EA, Beck M. (2013)
Fourier ring correlation as a resolution criterion for super-resolution microscopy
J. Struct. Biol. 183(3):363-367. doi:10.1016/j.jsb.2013.05.004
Europe PMC | doi

Herner A, Nikić I, Kállay M, Lemke EA, Kele P. (2013)
A new family of bioorthogonally applicable fluorogenic labels
Org. Biomol. Chem. 11(20):3297-3306. doi:10.1039/c3ob40296g
Europe PMC | doi

Lukinavičius G, Umezawa K, Olivier N, Honigmann A, Yang G, Plass T, Mueller V, Reymond L, Corrêa IR Jr, Luo ZG, Schultz C, Lemke EA, Heppenstall P, Eggeling C, Manley S, Johnsson K. (2013)
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Nat Chem 5(2):132-139. doi:10.1038/nchem.1546
Europe PMC | doi

Milles S, Huy Bui K, Koehler C, Eltsov M, Beck M, Lemke EA. (2013)
Facilitated aggregation of FG nucleoporins under molecular crowding conditions
EMBO Rep. 14(2):178-183. doi:10.1038/embor.2012.204
Europe PMC | doi

Ori A, Banterle N, Iskar M, Andrés-Pons A, Escher C, Khanh Bui H, Sparks L, Solis-Mezarino V, Rinner O, Bork P, Lemke EA, Beck M. (2013)
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines
Mol. Syst. Biol. 9:648. doi:10.1038/msb.2013.4
Europe PMC | doi

Tyagi S, Lemke EA. (2013)
Genetically encoded click chemistry for single-molecule FRET of proteins
Methods Cell Biol. 113:169-187. doi:10.1016/b978-0-12-407239-8.00009-4
Europe PMC | doi

Milles S, Lemke EA. (2013)
What precision-protein-tuning and nano-resolved single molecule sciences can do for each other
Bioessays 35(1):65-74. doi:10.1002/bies.201200094
Europe PMC | doi


2012

Milles S, Koehler C, Gambin Y, Deniz AA, Lemke EA. (2012)
Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range
Mol Biosyst 8(10):2531-2534. doi:10.1039/c2mb25135c
Europe PMC | doi

Borrmann A, Milles S, Plass T, Dommerholt J, Verkade JM, Wiessler M, Schultz C, van Hest JC, van Delft FL, Lemke EA. (2012)
Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation
Chembiochem 13(14):2094-2099. doi:10.1002/cbic.201200407
Europe PMC | doi

Krishnan R, Goodman JL, Mukhopadhyay S, Pacheco CD, Lemke EA, Deniz AA, Lindquist S. (2012)
Conserved features of intermediates in amyloid assembly determine their benign or toxic states
Proc. Natl. Acad. Sci. U.S.A. 109(28):11172-11177. doi:10.1073/pnas.1209527109
Europe PMC | doi

Plass T, Milles S, Koehler C, Szymański J, Mueller R, Wiessler M, Schultz C, Lemke EA. (2012)
Amino acids for Diels-Alder reactions in living cells
Angew. Chem. Int. Ed. Engl. 51(17):4166-4170. doi:10.1002/anie.201108231
Europe PMC | doi

Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA. (2012)
Click strategies for single-molecule protein fluorescence
J. Am. Chem. Soc. 134(11):5187-5195. doi:10.1021/ja210587q
Europe PMC | doi

Plass T, Milles S, Koehler C, Schultz C, Lemke EA. (2012)
Labeling Freedom for the Single Molecule Microscopist
Biophys J 102(3 Suppl 1):217A. doi:10.1016/j.bpj.2011.11.1187
doi


2011

Milles S, Lemke EA. (2011)
Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153
Biophys. J. 101(7):1710-1719. doi:10.1016/j.bpj.2011.08.025
Europe PMC | doi

Lemke EA, Schultz C. (2011)
Principles for designing fluorescent sensors and reporters
Nat. Chem. Biol. 7(8):480-483. doi:10.1038/nchembio.620
Europe PMC | doi

Plass T, Milles S, Koehler C, Schultz C, Lemke EA. (2011)
Genetically encoded copper-free click chemistry
Angew. Chem. Int. Ed. Engl. 50(17):3878-3881. doi:10.1002/anie.201008178
Europe PMC | doi

Gambin Y, VanDelinder V, Ferreon AC, Lemke EA, Groisman A, Deniz AA. (2011)
Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing
Nat. Methods 8(3):239-241. doi:10.1038/nmeth.1568
Europe PMC | doi

Lemke EA. (2011)
Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities
Methods Mol. Biol. 751:3-15. doi:10.1007/978-1-61779-151-2_1
Europe PMC | doi


2010

Lemke EA. (2010)
Precision control of cellular pathways with light
Chembiochem 11(13):1825-1827. doi:10.1002/cbic.201000364
Europe PMC | doi


2009

Lee HS, Guo J, Lemke EA, Dimla RD, Schultz PG. (2009)
Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae
J. Am. Chem. Soc. 131(36):12921-12923. doi:10.1021/ja904896s
Europe PMC | doi

Lemke EA, Gambin Y, Vandelinder V, Brustad EM, Liu HW, Schultz PG, Groisman A, Deniz AA. (2009)
Microfluidic device for single-molecule experiments with enhanced photostability
J. Am. Chem. Soc. 131(38):13610-13612. doi:10.1021/ja9027023
Europe PMC | doi

Gambin Y, Schug A, Lemke EA, Lavinder JJ, Ferreon AC, Magliery TJ, Onuchic JN, Deniz AA. (2009)
Direct single-molecule observation of a protein living in two opposed native structures
Proc. Natl. Acad. Sci. U.S.A. 106(25):10153-10158. doi:10.1073/pnas.0904461106
Europe PMC | doi

Ferreon AC, Gambin Y, Lemke EA, Deniz AA. (2009)
Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence
Proc. Natl. Acad. Sci. U.S.A. 106(14):5645-5650. doi:10.1073/pnas.0809232106
Europe PMC | doi


2008

Brustad EM, Lemke EA, Schultz PG, Deniz AA. (2008)
A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer
J. Am. Chem. Soc. 130(52):17664-17665. doi:10.1021/ja807430h
Europe PMC | doi

Deniz AA, Mukhopadhyay S, Lemke EA. (2008)
Single-molecule biophysics: at the interface of biology, physics and chemistry
J R Soc Interface 5(18):15-45. doi:10.1098/rsif.2007.1021
Europe PMC | doi


2007

Lemke EA, Summerer D, Geierstanger BH, Brittain SM, Schultz PG. (2007)
Control of protein phosphorylation with a genetically encoded photocaged amino acid
Nat. Chem. Biol. 3(12):769-772. doi:10.1038/nchembio.2007.44
Europe PMC | doi

Henkel AW, Dittrich PS, Groemer TW, Lemke EA, Klingauf J, Klafki HW, Lewczuk P, Esselmann H, Schwille P, Kornhuber J, Wiltfang J. (2007)
Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients
Mol. Psychiatry 12(6):601-610. doi:10.1038/sj.mp.4001947
Europe PMC | doi

Mukhopadhyay S, Krishnan R, Lemke EA, Lindquist S, Deniz AA. (2007)
A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures
Proc. Natl. Acad. Sci. U.S.A. 104(8):2649-2654. doi:10.1073/pnas.0611503104
Europe PMC | doi


2006

Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. (2006)
Molecular anatomy of a trafficking organelle
Cell 127(4):831-846. doi:10.1016/j.cell.2006.10.030
Europe PMC | doi


2005

Lemke EA, Klingauf J. (2005)
Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity
J. Neurosci. 25(47):11034-11044. doi:10.1523/jneurosci.2971-05.2005
Europe PMC | doi

Jordan R, Lemke EA, Klingauf J. (2005)
Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy
Biophys. J. 89(3):2091-2102. doi:10.1529/biophysj.105.061663
Europe PMC | doi


2003

Chakraborty R, Lemke EA, Cao Z, Klebba PE, van der Helm D. (2003)
Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli
Biometals 16(4):507-518. doi:10.1023/a:1023485632520
Europe PMC | doi