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Advanced Light Microscopy FacilityBooking and Equipment

Widefield Microscopes

Microscope Features Room
API Deltavision RT
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motorized high-precision xyz-stage, filter wheels, deconvolution, environment box 13-515A
Eppendorf Microinjection
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Widefield microscope with Eppendorf microinjection devices 13-515C
Lambert LIFA FLIM
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FLIM, FRET, environment box, motorized xy-stage
13-514A
Leica AF7000 TIRF
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Fast filterwheels, multi-color TIRF (405, 488, 561, 640 nm laser), FRET, environment box, adaptive feedback microscopy, EMCCD, motorized xy-stage 13-509A
Olympus Cut^R
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Laser nanosurgery (355 nm with <1 ps pulse width controlled via Rapp Optoelctronic scanner), environment box, microinjection, motorized xy-stage 13-509E
Olympus Scan^R
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Motorized xy-stage, environment box, Cell^R (experiment manager) and Scan^R (high-throughput) software 13-514C
Olympus Cell^R TIRF
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EMCCD, multi-color TIRF (405, 488, 561, 640 nm laser), Environment box (cellVivo), filterwheels, motorized xy-stage, hardware autofocus (ZDC3) 13-515E
Olympus LV200
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bioluminescence and fluorescence detection, light shielding environment box, 13-515B
Olympus MVX
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Macro zoom 13-515B
Zeiss Axiovert 200
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Standard widefield fluorescence microscope, motorized xy-stage 13-515C
Zeiss Cellobserver
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Environment box, Lumen Dynamics 120LED light source, motorized xy-stage 13-515D
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Confocal Microscopes

Microscope Features Room

Leica SP2
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Confocal laser scanning microscope, FCS, FRAP, FRET, lasers: 405, 458, 476, 488, 496, 514, 561, 633 nm, environment box, motorized xy-stage 13-509C
Leica SP8 STED 3X
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Confocal laser scanning microscope with white-light laser (470-670 nm) and gated detection,
stimulated emission depletion (STED) 3D super-resolution, environment box, motorized xy-stage
13-516B
Leica SP8 CSU
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Confocal laser scanning microscope, FRAP, FRET, filter-based confocal laser scanning microscope, available laser wavelengths: 405, 488, 552, 640 nm, motorized xy-stage 13-514B
Olympus FV1200
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Confocal laser scanning microscope with two scanners, second scanner with pulsed lasers (355, 375, 532 nm) for nano-surgery and photo-uncagingFRAP, FRET, photo-activation, environment box, hardware autofocus (ZDC3) 13-516E
PE Ultraview ERS
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Spinning disk confocal microscope, FRET, lasers: 405, 488, 514, 561, 640 nm, environment box, motorized xy-stage
13-508D
PE Ultraview VoX
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Spinning disk confocal microscope, photokinesis unit (FRAP, photo-activation), FRET, environment box, lasers: 405, 440, 488, 514, 568, 640 nm, motorized xy-stage 13-514D
Zeiss LSM 510 META
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Confocal laser scanning microscope with spectral detector, environment box, microinjection, motorized xy-stage 13-509B
Zeiss LSM 780
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Confocal laser scanning microscope with GaAsP spectral detector, FCS, FRAP, FRET lasers: 405, 458, 488, 514, 561, 633 nm, environment box, hardware autofocus (definite focus), motorized xy-stage 13-516C
Zeiss LSM 780 NLO
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2-photon (can be used for nano-surgery and photo-uncaging) and confocal laser scanning microscope, FCS, FRAP, FRET,  GaAsP external detectors, spectral GaAsP detection, lasers: 405, 458, 488, 514, 561, 594, 633 nm, 690-1064 nm(2-photon laser), environment box, hardware autofocus (definite focus), motorized xy-stage 13-516D
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Super-Resolution Microscopes

Microscope Features Room
Leica GSDIM
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Localization based super-resolution in 3D by ground state depletion by individual return microscopy (GSDIM, also called dSTORM) 13-509D
Leica SP8 STED 3X
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3D stimulated emission depletion (STED) microscopy with gated detection, environment box, motorized xy-stage, lasers: 405 nm, Ar-laser (458, 476, 488, 496, 514 nm), white-light laser (470-670 nm), STED laser (592 + 660 nm) 13-516B
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High-throughput microscopy

High-throughput equipment can be booked via our project management system (internal access only).

Microscope Features Room

Leica SP5 MSA (A)
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 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508F

Leica SP5 MSA (B)
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 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508E
Molecular Devices IXM
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Widefield epifluorescence and transmission microscope, sCMOS camera, Environment box, automated pipetting 13-514E
Olympus ScanR (cube2)
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Widefield epifluorescence and transmission microscope, environment box 13-508B
Olympus ScanR (cube3)
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Widefield epifluorescence and transmission microscope, environment box, frequency domain FLIM 13-514C
Olympus ScanR (cube4)
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Widefield epifluorescence and transmission microscope, environment box 13-508B
Olympus ScanR (cube5)
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Widefield epifluorescence and transmission microscope, environment box, hardware autofocus 13-508B
Olympus ScanR (cube6)
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Widefield epifluorescence and transmission microscope, environment box 13-508B
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Special Objectives

Company Magnification and NA Immersion type
Leica
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63x 1.2 (for SP2) water
Leica
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63x 1.2 (for SP5) water
Olympus
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30x 1.05 silicon
Olympus
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40x 1.15 water
Olympus
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60x 1.2 water
Zeiss
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5x 0.16 air
Zeiss
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40x 1.1 water
Zeiss
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40x 1.2 water
Zeiss
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63x 1.2 water
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Workstations

Equipment Installed Software Room
Workstation 1
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Volocity, Amira, Zen, Cell Profiler, Fiji 13-501
Workstation 2 
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Imaris, Cell Profiler, Fiji 13-501
Workstation 3 
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Leica LASAF, Cell Profiler, Fiji 13-501
Workstation 4 
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Matlab, Cell Profiler, Fiji 13-501

Additional Equipment

Equipment Features Room
Cell culture hood
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For splitting cells and general cell culture work 13-505
Cell seeding device
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Automated cell seeding - 96 and 384 well plates 13-505
Liquidator (cell culture)
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Manual 96 channel pipetting system - for cell staining / media change 13-505
Liquidator (lab)
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Manual 96 channel pipetting system - for siRNA coating only 13-507
Speed Vac (MiVac A)
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Vacuum concentrator for 96 and 384 well plates 13-507
Speed Vac (MiVac B)
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Vacuum concentrator for 96 and 384 well plates 13-507
small heating chamber
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small stage top environment chamber suitable for Maerzhaeuser stages. 13-502
Sutter needle puller Device to prepare capillaries for microinjection 13-515C

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Room Label Phone number
13-509 cherry 8608
13-514 orange 8646
13-515 banana 8647
13-516 plum 8649
13-501 visitor office 8629
     
overview building 13 floor 5
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