Staff at EMBL

Dr. Beate Neumann

Senior Scientific Officer
Advanced Light Microscopy Facility

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Publications

TRIP12 and UBR5 Suppress Spreading of Chromatin Ubiquitylation at Damaged Chromosomes. Gudjonsson, T., Altmeyer, M., Savic, V., Toledo, L., Dinant, C., Grofte, M., Bartkova, J., Poulsen, M., Oka, Y., Bekker-Jensen, S., Mailand, N., Neumann, B., Heriche, J.K., Shearer, R., Saunders, D., Bartek, J., Lukas, J. & Lukas, C. Cell. 2012 Aug 7. PubMed

Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway. Simpson, J.C., Joggerst, B., Laketa, V., Verissimo, F., Cetin, C., Erfle, H., Bexiga, M.G., Singan, V.R., Heriche, J.K., Neumann, B., Mateos, A., Blake, J., Bechtel, S., Benes, V., Wiemann, S., Ellenberg, J. & Pepperkok, R. Nat Cell Biol. 2012 Jun 3;14(7):764-74. doi: 10.1038/ncb2510. PubMed

Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1. Moudry, P., Lukas, C., Macurek, L., Neumann, B., Heriche, J.K., Pepperkok, R., Ellenberg, J., Hodny, Z., Lukas, J. & Bartek, J. Cell Death Differ. 2012 May;19(5):798-807. doi: 10.1038/cdd.2011.150. Epub 2011Nov 11. PubMed

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. Stadler, C., Hjelmare, M., Neumann, B., Jonasson, K., Pepperkok, R., Uhlen, M. & Lundberg, E. J Proteomics. 2012 Feb 15. PubMed

Automated High-Throughput RNAi Screening in Human Cells Combined with Reporter mRNA Transfection to Identify Novel Regulators of Translation. Casanova, C.M., Sehr, P., Putzker, K., Hentze, M.W., Neumann, B., Duncan, K.E. & Thoma, C. PLoS One. 2012;7(9):e45943. doi: 10.1371/journal.pone.0045943. Epub 2012 Sep 27. PubMed

Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Ritzerfeld, J., Remmele, S., Wang, T., Temmerman, K., Brugger, B., Wegehingel, S., Tournaviti, S., Strating, J.R., Wieland, F.T., Neumann, B., Ellenberg, J., Lawerenz, C., Hesser, J., Erfle, H., Pepperkok, R. & Nickel, W. Genome Res. 2011 Nov;21(11):1955-68. doi: 10.1101/gr.116087.110. Epub 2011 Jul27. PubMed

53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress. Lukas, C., Savic, V., Bekker-Jensen, S., Doil, C., Neumann, B., Solvhoj Pedersen, R., Grofte, M., Chan, K.L., Hickson, I.D., Bartek, J. & Lukas, J. Nat Cell Biol. 2011 Mar;13(3):243-53. Epub 2011 Feb 13. PubMed

CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held, M., Schmitz, M.H., Fischer, B., Walter, T., Neumann, B., Olma, M.H., Peter, M., Ellenberg, J. & Gerlich, D.W. Nat Methods. 2010 Sep;7(9):747-54. doi: 10.1038/nmeth.1486. Epub 2010 Aug 8. PubMed

Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging. Walter, T., Held, M., Neumann, B., Heriche, J.K., Conrad, C., Pepperkok, R. & Ellenberg, J. J Struct Biol. 2010 Apr;170(1):1-9. doi: 10.1016/j.jsb.2009.10.004. Epub 2009 Oct23. PubMed

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J. Nature. 2010 Apr 1;464(7289):721-7. PubMed

High-Throughput Microscopy Using Live Mammalian Cells. Terjung, S., Walter, T., Seitz, A., Neumann, B., Pepperkok, R. & Ellenberg, J. In 'Live Cell Imaging: A Laboratory Manual', Second Edition, 2010 (eds. R.D. Goldman, J.R. Swedlow & D.L. Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor

Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. Erfle, H., Neumann, B., Rogers, P., Bulkescher, J., Ellenberg, J. & Pepperkok, R. J Biomol Screen. 2008 Aug;13(7):575-80. Epub 2008 Jul 3. PubMed

EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. Tegha-Dunghu, J., Neumann, B., Reber, S., Krause, R., Erfle, H., Walter, T., Held, M., Rogers, P., Hupfeld, K., Ruppert, T., Ellenberg, J. & Gruss, O.J. J Cell Sci. 2008 May 15;121(Pt 10):1718-26. Epub 2008 Apr 29. PubMed

Reverse transfection on cell arrays for high content screening microscopy. Erfle, H., Neumann, B., Liebel, U., Rogers, P., Held, M., Walter, T., Ellenberg, J. & Pepperkok, R. Nat Protoc. 2007;2(2):392-9. PubMed

High-throughput RNAi screening by time-lapse imaging of live human cells. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R. & Ellenberg, J. Nat Methods. 2006 May;3(5):385-90. PubMed

Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Sonnichsen, B., Koski, L.B., Walsh, A., Marschall, P., Neumann, B., Brehm, M., Alleaume, A.M., Artelt, J., Bettencourt, P., Cassin, E., Hewitson, M., Holz, C., Khan, M., Lazik, S., Martin, C., Nitzsche, B., Ruer, M., Stamford, J., Winzi, M., Heinkel, R., Roder, M., Finell, J., Hantsch, H., Jones, S.J., Jones, M., Piano, F., Gunsalus, K.C., Oegema, K., Gonczy, P., Coulson, A., Hyman, A.A. & Echeverri, C.J. Nature 2005 Mar 24;434(7032):462-9. PubMed

RGD-mutants of B-lymphotropic polyomavirus capsids specifically bind to alpha(v)beta3 integrin. Langner, J., Neumann, B., Goodman, S.L. & Pawlita, M. Arch Virol 2004 Oct;149(10):1877-96. PubMed