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Staff at EMBL


Dr. Rainer Pepperkok

Head of Core Facilities, Head of Advanced Light Microscopy Core Facility, Team Leader and Senior Scientist
Core Facilities Pepperkok Team Advanced Light Microscopy Facility

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

E-mail: send a mail


PhD 1992, University Kaiserslautern.
Postdoctoral research at University of Geneva.
Lab head at the Imperial Cancer Research Fund, London.
At EMBL since 1998.
Senior scientist since 2012.
Head of Core Facilities and Scientific Services since 2014.


Total: 167 publication(s)


Ronchi P, Tischer C, Acehan D, Pepperkok R. (2014)
Positive feedback between golgi membranes, microtubules and ER-exit sites directs golgi de novo biogenesis.
J. Cell. Sci. doi:10.1242/jcs.150474
Europe PMC | doi

Eskova A, Knapp B, Matelska D, Reusing S, Arjonen A, Lisauskas T, Pepperkok R, Russell R, Eils R, Ivaska J, Kaderali L, Erfle H, Starkuviene V. (2014)
An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin.
J. Cell. Sci. 127(Pt 11):2433-2447. doi:10.1242/jcs.137281
Europe PMC | doi

Sauvageau E, Rochdi MD, Oueslati M, Hamdan FF, Percherancier Y, Simpson JC, Pepperkok R, Bouvier M. (2014)
CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum
Traffic 15(4):383-400. doi:10.1111/tra.12148
Europe PMC | doi

Michel G, Matthes HW, Hachet-Haas M, El Baghdadi K, de Mey J, Pepperkok R, Simpson JC, Galzi JL, Lecat S. (2014)
Plasma membrane translocation of REDD1 governed by GPCRs contributes to mTORC1 activation
J. Cell. Sci. 127(Pt 4):773-787. doi:10.1242/jcs.136432
Europe PMC | doi

Laketa V, Zarbakhsh S, Traynor-Kaplan A, Macnamara A, Subramanian D, Putyrski M, Mueller R, Nadler A, Mentel M, Saez-Rodriguez J, Pepperkok R, Schultz C. (2014)
PIP₃ induces the recycling of receptor tyrosine kinases
Sci Signal 7(308):ra5. doi:10.1126/scisignal.2004532
Europe PMC | doi

Tischer C, Hilsenstein V, Hanson K, Pepperkok R. (2014)
Adaptive fluorescence microscopy by online feedback image analysis.
Methods Cell Biol. 123:489-503. doi:10.1016/b978-0-12-420138-5.00026-4
Europe PMC | doi


Verissimo F, Pepperkok R. (2013)
Imaging ER-to-Golgi transport: towards a systems view
J. Cell. Sci. 126(Pt 22):5091-5100. doi:10.1242/jcs.121061
Europe PMC | doi

Botelho HM, Dahimene S, Uliyakina I, Neumann B, Tischer C, Pepperkok R, Amaral MD. (2013)

He S, Ni D, Ma B, Lee JH, Zhang T, Ghozalli I, Pirooz SD, Zhao Z, Bharatham N, Li B, Oh S, Lee WH, Takahashi Y, Wang HG, Minassian A, Feng P, Deretic V, Pepperkok R, Tagaya M, Yoon HS, Liang C. (2013)
PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin 1 complex
Nat. Cell Biol. 15(10):1206-1219. doi:10.1038/ncb2848
Europe PMC | doi

Almaça J, Faria D, Sousa M, Uliyakina I, Conrad C, Sirianant L, Clarke LA, Martins JP, Santos M, Heriché JK, Huber W, Schreiber R, Pepperkok R, Kunzelmann K, Amaral MD. (2013)
High-content siRNA screen reveals global ENaC regulators and potential cystic fibrosis therapy targets
Cell 154(6):1390-1400. doi:10.1016/j.cell.2013.08.045
Europe PMC | doi

Gerdes HH, Pepperkok R. (2013)
Cell-to-cell communication: current views and future perspectives
Cell Tissue Res. 352(1):1-3. doi:10.1007/s00441-013-1590-1
Europe PMC | doi

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E. (2013)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Nat. Methods 10(4):315-323. doi:10.1038/nmeth.2377
Europe PMC | doi

Iskar M, Zeller G, Blattmann P, Campillos M, Kuhn M, Kaminska KH, Runz H, Gavin AC, Pepperkok R, van Noort V, Bork P. (2013)
Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding
Mol. Syst. Biol. 9:662. doi:10.1038/msb.2013.20
Europe PMC | doi

Ronchi P, Pepperkok R. (2013)
Golgi depletion from living cells with laser nanosurgery
Methods Cell Biol. 118:311-324. doi:10.1016/b978-0-12-417164-0.00019-7
Europe PMC | doi

Blattmann P, Schuberth C, Pepperkok R, Runz H. (2013)
RNAi-based functional profiling of loci from blood lipid genome-wide association studies identifies genes with cholesterol-regulatory function
PLoS Genet. 9(2):e1003338. doi:10.1371/journal.pgen.1003338
Europe PMC | doi


Simpson JC, Joggerst B, Laketa V, Verissimo F, Cetin C, Erfle H, Bexiga MG, Singan VR, Hériché JK, Neumann B, Mateos A, Blake J, Bechtel S, Benes V, Wiemann S, Ellenberg J, Pepperkok R. (2012)
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway
Nat. Cell Biol. 14(7):764-774. doi:10.1038/ncb2510
Europe PMC | doi

Moudry P, Lukas C, Macurek L, Neumann B, Heriche JK, Pepperkok R, Ellenberg J, Hodny Z, Lukas J, Bartek J. (2012)
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1
Cell Death Differ. 19(5):798-807. doi:10.1038/cdd.2011.150
Europe PMC | doi

Ronchi P, Terjung S, Pepperkok R. (2012)
At the cutting edge: applications and perspectives of laser nanosurgery in cell biology
Biol. Chem. 393(4):235-248. doi:10.1515/hsz-2011-0237
Europe PMC | doi

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E. (2012)
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
J Proteomics 75(7):2236-2251. doi:10.1016/j.jprot.2012.01.030
Europe PMC | doi

Taverna E, Haffner C, Pepperkok R, Huttner WB. (2012)
A new approach to manipulate the fate of single neural stem cells in tissue
Nat. Neurosci. 15(2):329-337. doi:10.1038/nn.3008
Europe PMC | doi

Roth SD, Schüttrumpf J, Milanov P, Abriss D, Ungerer C, Quade-Lyssy P, Simpson JC, Pepperkok R, Seifried E, Tonn T. (2012)
Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia A
PLoS ONE 7(9):e44505. doi:10.1371/journal.pone.0044505
Europe PMC | doi

Contreras FX, Ernst AM, Haberkant P, Björkholm P, Lindahl E, Gönen B, Tischer C, Elofsson A, von Heijne G, Thiele C, Pepperkok R, Wieland F, Brügger B. (2012)
Molecular recognition of a single sphingolipid species by a protein's transmembrane domain
Nature 481(7382):525-529. doi:10.1038/nature10742
Europe PMC | doi

Seitz A, Terjung S, Zimmermann T, Pepperkok R. (2012)
Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements
J Biomed Opt 17(1):011010. doi:10.1117/1.jbo.17.1.011010
Europe PMC | doi


Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R, Nickel W. (2011)
Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases
Genome Res. 21(11):1955-1968. doi:10.1101/gr.116087.110
Europe PMC | doi

Moore HM, Bai B, Boisvert FM, Latonen L, Rantanen V, Simpson JC, Pepperkok R, Lamond AI, Laiho M. (2011)
Quantitative proteomics and dynamic imaging of the nucleolus reveal distinct responses to UV and ionizing radiation
Mol. Cell Proteomics 10(10):M111.009241. doi:10.1074/mcp.m111.009241
Europe PMC | doi

Tängemo C, Ronchi P, Colombelli J, Haselmann U, Simpson JC, Antony C, Stelzer EH, Pepperkok R, Reynaud EG. (2011)
A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells
J. Cell. Sci. 124(Pt 6):978-987. doi:10.1242/jcs.079640
Europe PMC | doi

Conrad C, Wünsche A, Tan TH, Bulkescher J, Sieckmann F, Verissimo F, Edelstein A, Walter T, Liebel U, Pepperkok R, Ellenberg J. (2011)
Micropilot: automation of fluorescence microscopy-based imaging for systems biology
Nat. Methods 8(3):246-249. doi:10.1038/nmeth.1558
Europe PMC | doi

Almaça J, Dahimène S, Appel N, Conrad C, Kunzelmann K, Pepperkok R, Amaral MD. (2011)
Functional genomics assays to study CFTR traffic and ENaC function
Methods Mol. Biol. 742:249-264. doi:10.1007/978-1-61779-120-8_15
Europe PMC | doi

Reiss S, Rebhan I, Backes P, Romero-Brey I, Erfle H, Matula P, Kaderali L, Poenisch M, Blankenburg H, Hiet MS, Longerich T, Diehl S, Ramirez F, Balla T, Rohr K, Kaul A, Bühler S, Pepperkok R, Lengauer T, Albrecht M, Eils R, Schirmacher P, Lohmann V, Bartenschlager R. (2011)
Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment
Cell Host Microbe 9(1):32-45. doi:10.1016/j.chom.2010.12.002
Europe PMC | doi


Terjung S, Walter T, Seitz A, Neumann B, Pepperkok R, Ellenberg J. (2010)
High-throughput microscopy using live mammalian cells
Cold Spring Harb Protoc 2010(7):pdb.top84. doi:10.1101/pdb.top84
Europe PMC | doi

Grecco HE, Roda-Navarro P, Girod A, Hou J, Frahm T, Truxius DC, Pepperkok R, Squire A, Bastiaens PI. (2010)
In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays
Nat. Methods 7(6):467-472. doi:10.1038/nmeth.1458
Europe PMC | doi

Ebert AD, Laussmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R, Nickel W. (2010)
Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion
Traffic 11(6):813-826. doi:10.1111/j.1600-0854.2010.01059.x
Europe PMC | doi

Subramanian D, Laketa V, Müller R, Tischer C, Zarbakhsh S, Pepperkok R, Schultz C. (2010)
Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
Nat. Chem. Biol. 6(5):324-326. doi:10.1038/nchembio.348
Europe PMC | doi

Walter T, Held M, Neumann B, Hériché JK, Conrad C, Pepperkok R, Ellenberg J. (2010)
Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging
J. Struct. Biol. 170(1):1-9. doi:10.1016/j.jsb.2009.10.004
Europe PMC | doi

Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J. (2010)
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Nature 464(7289):721-727. doi:10.1038/nature08869
Europe PMC | doi

Börner K, Hermle J, Sommer C, Brown NP, Knapp B, Glass B, Kunkel J, Torralba G, Reymann J, Beil N, Beneke J, Pepperkok R, Schneider R, Ludwig T, Hausmann M, Hamprecht F, Erfle H, Kaderali L, Kräusslich HG, Lehmann MJ. (2010)
From experimental setup to bioinformatics: an RNAi screening platform to identify host factors involved in HIV-1 replication
Biotechnol J 5(1):39-49. doi:10.1002/biot.200900226
Europe PMC | doi


Heinz S, Schüttrumpf J, Simpson JC, Pepperkok R, Nicolaes GA, Abriss D, Milanov P, Roth S, Seifried E, Tonn T. (2009)
Factor VIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of factor VIII
Thromb. Haemost. 102(5):925-935. doi:10.1160/th08-12-0807
Europe PMC | doi

Laketa V, Zarbakhsh S, Morbier E, Subramanian D, Dinkel C, Brumbaugh J, Zimmermann P, Pepperkok R, Schultz C. (2009)
Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth
Chem. Biol. 16(11):1190-1196. doi:10.1016/j.chembiol.2009.10.005
Europe PMC | doi

Gilbert DF, Meinhof T, Pepperkok R, Runz H. (2009)
DetecTiff: a novel image analysis routine for high-content screening microscopy
J Biomol Screen 14(8):944-955. doi:10.1177/1087057109339523
Europe PMC | doi

Schwarz K, Iolascon A, Verissimo F, Trede NS, Horsley W, Chen W, Paw BH, Hopfner KP, Holzmann K, Russo R, Esposito MR, Spano D, De Falco L, Heinrich K, Joggerst B, Rojewski MT, Perrotta S, Denecke J, Pannicke U, Delaunay J, Pepperkok R, Heimpel H. (2009)
Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II
Nat. Genet. 41(8):936-940. doi:10.1038/ng.405
Europe PMC | doi

Tournaviti S, Pietro ES, Terjung S, Schafmeier T, Wegehingel S, Ritzerfeld J, Schulz J, Smith DF, Pepperkok R, Nickel W. (2009)
Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins
Traffic 10(8):1047-1060. doi:10.1111/j.1600-0854.2009.00921.x
Europe PMC | doi

Bartz F, Kern L, Erz D, Zhu M, Gilbert D, Meinhof T, Wirkner U, Erfle H, Muckenthaler M, Pepperkok R, Runz H. (2009)
Identification of cholesterol-regulating genes by targeted RNAi screening
Cell Metab. 10(1):63-75. doi:10.1016/j.cmet.2009.05.009
Europe PMC | doi

Martinez-Morales JR, Rembold M, Greger K, Simpson JC, Brown KE, Quiring R, Pepperkok R, Martin-Bermudo MD, Himmelbauer H, Wittbrodt J. (2009)
ojoplano-mediated basal constriction is essential for optic cup morphogenesis
Development 136(13):2165-2175. doi:10.1242/dev.033563
Europe PMC | doi

Doil C, Mailand N, Bekker-Jensen S, Menard P, Larsen DH, Pepperkok R, Ellenberg J, Panier S, Durocher D, Bartek J, Lukas J, Lukas C. (2009)
RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins
Cell 136(3):435-446. doi:10.1016/j.cell.2008.12.041
Europe PMC | doi


Dejgaard SY, Murshid A, Erman A, Kizilay O, Verbich D, Lodge R, Dejgaard K, Ly-Hartig TB, Pepperkok R, Simpson JC, Presley JF. (2008)
Rab18 and Rab43 have key roles in ER-Golgi trafficking
J. Cell. Sci. 121(Pt 16):2768-2781. doi:10.1242/jcs.021808
Europe PMC | doi

Erfle H, Neumann B, Rogers P, Bulkescher J, Ellenberg J, Pepperkok R. (2008)
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
J Biomol Screen 13(7):575-580. doi:10.1177/1087057108320133
Europe PMC | doi

Pouthas F, Girard P, Lecaudey V, Ly TB, Gilmour D, Boulin C, Pepperkok R, Reynaud EG. (2008)
In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum
J. Cell. Sci. 121(Pt 14):2406-2414. doi:10.1242/jcs.026849
Europe PMC | doi

Mathivanan S, Ahmed M, Ahn NG, Alexandre H, Amanchy R, Andrews PC, Bader JS, Balgley BM, Bantscheff M, Bennett KL, Björling E, Blagoev B, Bose R, Brahmachari SK, Burlingame AS, Bustelo XR, Cagney G, Cantin GT, Cardasis HL, Celis JE, Chaerkady R, Chu F, Cole PA, Costello CE, Cotter RJ, Crockett D, DeLany JP, De Marzo AM, DeSouza LV, Deutsch EW, Dransfield E, Drewes G, Droit A, Dunn MJ, Elenitoba-Johnson K, Ewing RM, Van Eyk J, Faca V, Falkner J, Fang X, Fenselau C, Figeys D, Gagné P, Gelfi C, Gevaert K, Gimble JM, Gnad F, Goel R, Gromov P, Hanash SM, Hancock WS, Harsha HC, Hart G, Hays F, He F, Hebbar P, Helsens K, Hermeking H, Hide W, Hjernø K, Hochstrasser DF, Hofmann O, Horn DM, Hruban RH, Ibarrola N, James P, Jensen ON, Jensen PH, Jung P, Kandasamy K, Kheterpal I, Kikuno RF, Korf U, Körner R, Kuster B, Kwon MS, Lee HJ, Lee YJ, Lefevre M, Lehvaslaiho M, Lescuyer P, Levander F, Lim MS, Löbke C, Loo JA, Mann M, Martens L, Martinez-Heredia J, McComb M, McRedmond J, Mehrle A, Menon R, Miller CA, Mischak H, Mohan SS, Mohmood R, Molina H, Moran MF, Morgan JD, Moritz R, Morzel M, Muddiman DC, Nalli A, Navarro JD, Neubert TA, Ohara O, Oliva R, Omenn GS, Oyama M, Paik YK, Pennington K, Pepperkok R, Periaswamy B, Petricoin EF, Poirier GG, Prasad TS, Purvine SO, Rahiman BA, Ramachandran P, Ramachandra YL, Rice RH, Rick J, Ronnholm RH, Salonen J, Sanchez JC, Sayd T, Seshi B, Shankari K, Sheng SJ, Shetty V, Shivakumar K, Simpson RJ, Sirdeshmukh R, Siu KW, Smith JC, Smith RD, States DJ, Sugano S, Sullivan M, Superti-Furga G, Takatalo M, Thongboonkerd V, Trinidad JC, Uhlen M, Vandekerckhove J, Vasilescu J, Veenstra TD, Vidal-Taboada JM, Vihinen M, Wait R, Wang X, Wiemann S, Wu B, Xu T, Yates JR, Zhong J, Zhou M, Zhu Y, Zurbig P, Pandey A. (2008)
Human Proteinpedia enables sharing of human protein data
Nat. Biotechnol. 26(2):164-167. doi:10.1038/nbt0208-164
Europe PMC | doi

Colombelli J, Tängemo C, Haselman U, Antony C, Stelzer EH, Pepperkok R, Reynaud EG. (2008)
A correlative light and electron microscopy method based on laser micropatterning and etching
Methods Mol. Biol. 457:203-213. doi:10.1007/978-1-59745-261-8_15
Europe PMC | doi

Walter T, Held M, Neumann B, Heriche JK, Conrad C, Pepperkok R, Ellenberg J. (2008)
A genome wide RNAI screen by time lapse microscopy in order to identify mitotic genes - Computational aspects and challenges

Verissimo F, Pepperkok R. (2008)
ER-to-Golgi transport
In: Mironov AA & Pavelka M (eds.), The Golgi Apparatus: State of the art 110 years after Camillo Golgi's discovery. Springer Vienna. pp 333-341.doi:10.1007/978-3-211-76310-0_20

Pepperkok R, Wiemann S. (2008)
Integrating systems biology with clinical research
Genome Biol. 9(7):314. doi:10.1186/gb-2008-9-7-314
Europe PMC | doi


Starkuviene V, Pepperkok R. (2007)
The potential of high-content high-throughput microscopy in drug discovery
Br. J. Pharmacol. 152(1):62-71. doi:10.1038/sj.bjp.0707346
Europe PMC | doi

Starkuviene V, Pepperkok R. (2007)
Differential requirements for ts-O45-G and procollagen biosynthetic transport
Traffic 8(8):1035-1051. doi:10.1111/j.1600-0854.2007.00582.x
Europe PMC | doi

Starkuviene V, Pepperkok R, Erfle H. (2007)
Transfected cell microarrays: an efficient tool for high-throughput functional analysis
Expert Rev Proteomics 4(4):479-489. doi:10.1586/14789450.4.4.479
Europe PMC | doi

Simpson JC, Cetin C, Erfle H, Joggerst B, Liebel U, Ellenberg J, Pepperkok R. (2007)
An RNAi screening platform to identify secretion machinery in mammalian cells
Paper presented at the International Workshop on Molecular Systems Biology, Bielefeld, GERMANY, JUN, 2006.J. Biotechnol. 129(2):352-365. doi:10.1016/j.jbiotec.2006.12.027
Europe PMC | doi

von Brunn A, Teepe C, Simpson JC, Pepperkok R, Friedel CC, Zimmer R, Roberts R, Baric R, Haas J. (2007)
Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome
PLoS ONE 2(5):e459. doi:10.1371/journal.pone.0000459
Europe PMC | doi

Grabher C, Cliffe A, Miura K, Hayflick J, Pepperkok R, Rørth P, Wittbrodt J. (2007)
Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka
Paper presented at the EMBO Workshop on Innate Danger Signals and HMGB1, Milan, ITALY, FEB 08-11, 2006.J. Leukoc. Biol. 81(1):263-271. doi:10.1189/jlb.0806526
Europe PMC | doi

Laketa V, Simpson JC, Bechtel S, Wiemann S, Pepperkok R. (2007)
High-content microscopy identifies new neurite outgrowth regulators
Mol. Biol. Cell 18(1):242-252. doi:10.1091/mbc.e06-08-0666
Europe PMC | doi

Simpson JC, Mateos A, Pepperkok R. (2007)
Maturation of the mammalian secretome
Genome Biol. 8(4):211. doi:10.1186/gb-2007-8-4-211
Europe PMC | doi

Robinson DG, Herranz MC, Bubeck J, Pepperkok R, Ritzenthaler C. (2007)
Membrane dynamics in the early secretory pathway
CRC Crit Rev Plant Sci 26(4):199-225.
Europe PMC

Erfle H, Pepperkok R. (2007)
Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells
Methods Mol. Biol. 360:155-161. doi:10.1385/1-59745-165-7:155
Europe PMC | doi

Erfle H, Neumann B, Liebel U, Rogers P, Held M, Walter T, Ellenberg J, Pepperkok R. (2007)
Reverse transfection on cell arrays for high content screening microscopy
Nat Protoc 2(2):392-399. doi:10.1038/nprot.2006.483
Europe PMC | doi


Stauber T, Simpson JC, Pepperkok R, Vernos I. (2006)
A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
Curr. Biol. 16(22):2245-2251. doi:10.1016/j.cub.2006.09.060
Europe PMC | doi

Brunet S, Zimmermann T, Reynaud EG, Vernos I, Karsenti E, Pepperkok R. (2006)
Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion
Traffic 7(9):1283-1289. doi:10.1111/j.1600-0854.2006.00463.x
Europe PMC | doi

Pepperkok R, Ellenberg J. (2006)
High-throughput fluorescence microscopy for systems biology
Nat. Rev. Mol. Cell Biol. 7(9):690-696. doi:10.1038/nrm1979
Europe PMC | doi

Veríssimo F, Silva E, Morris JD, Pepperkok R, Jordan P. (2006)
Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway
Oncogene 25(30):4172-4182. doi:10.1038/sj.onc.1209449
Europe PMC | doi

Runz H, Miura K, Weiss M, Pepperkok R. (2006)
Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G
EMBO J. 25(13):2953-2965. doi:10.1038/sj.emboj.7601205
Europe PMC | doi

Tabary O, Boncoeur E, de Martin R, Pepperkok R, Clément A, Schultz C, Jacquot J. (2006)
Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells
Cell. Signal. 18(5):652-660. doi:10.1016/j.cellsig.2005.06.004
Europe PMC | doi

Neumann B, Held M, Liebel U, Erfle H, Rogers P, Pepperkok R, Ellenberg J. (2006)
High-throughput RNAi screening by time-lapse imaging of live human cells
Nat. Methods 3(5):385-390. doi:10.1038/nmeth876
Europe PMC | doi

Simpson JC, Nilsson T, Pepperkok R. (2006)
Biogenesis of tubular ER-to-Golgi transport intermediates
Mol. Biol. Cell 17(2):723-737. doi:10.1091/mbc.e05-06-0580
Europe PMC | doi

Colombelli J, Pepperkok R, Stelzer EH, Reynaud EG. (2006)
[Laser nanosurgery in cell biology]
Med Sci (Paris) 22(6-7):651-658. doi:10.1051/medsci/20062267651
Europe PMC | doi

Forster R, Weiss M, Zimmermann T, Reynaud EG, Verissimo F, Stephens DJ, Pepperkok R. (2006)
Secretory cargo regulates the turnover of COPII subunits at single ER exit sites
Curr. Biol. 16(2):173-179. doi:10.1016/j.cub.2005.11.076
Europe PMC | doi

Simpson JC, Pepperkok R. (2006)
Systematic Subcellular Localization of Novel Proteins
Third Edition edn. Academic Press.doi:10.1016/B978-012164730-8/50140-4

Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S. (2006)
The LIFEdb database in 2006
Nucleic Acids Res. 34(Database issue):D415-8. doi:10.1093/nar/gkj139
Europe PMC | doi

Simpson JC, Pepperkok R. (2006)
The subcellular localization of the mammalian proteome comes a fraction closer
Genome Biol. 7(6):222. doi:10.1186/gb-2006-7-6-222
Europe PMC | doi

Neumann B, Held M, Liebel U, Erfle H, Rogers P, Pepperkok R, Ellenberg J. (2006)
Time-lapse microscopy-based genome wide RNAI screening in live human cells


Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH. (2005)
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery
Traffic 6(12):1093-1102. doi:10.1111/j.1600-0854.2005.00334.x
Europe PMC | doi

Arlt D, Huber W, Liebel U, Schmidt C, Majety M, Sauermann M, Rosenfelder H, Bechtel S, Mehrle A, Bannasch D, Schupp I, Seiler M, Simpson JC, Hahne F, Moosmayer P, Ruschhaupt M, Guilleaume B, Wellenreuther R, Pepperkok R, Sültmann H, Poustka A, Wiemann S. (2005)
Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle
Cancer Res. 65(17):7733-7742. doi:10.1158/0008-5472.CAN-05-0642
Europe PMC | doi

Keese M, Magdeburg RJ, Herzog T, Hasenberg T, Offterdinger M, Pepperkok R, Sturm JW, Bastiaens PI. (2005)
Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues
J. Biol. Chem. 280(30):27826-27831. doi:10.1074/jbc.m504485200
Europe PMC | doi

Knauer SK, Moodt S, Berg T, Liebel U, Pepperkok R, Stauber RH. (2005)
Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions
Traffic 6(7):594-606. doi:10.1111/j.1600-0854.2005.00298.x
Europe PMC | doi

Yang YD, Elamawi R, Bubeck J, Pepperkok R, Ritzenthaler C, Robinson DG. (2005)
Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites
Plant Cell 17(5):1513-1531. doi:10.1105/tpc.104.026757
Europe PMC | doi

Neubrand VE, Will RD, Möbius W, Poustka A, Wiemann S, Schu P, Dotti CG, Pepperkok R, Simpson JC. (2005)
Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
EMBO J. 24(6):1122-1133. doi:10.1038/sj.emboj.7600600
Europe PMC | doi

Erfle H, Pepperkok R. (2005)
Arrays of transfected mammalian cells for high content screening microscopy
Meth. Enzymol. 404:1-8. doi:10.1016/s0076-6879(05)04001-2
Europe PMC | doi

Liebel U, Kindler B, Pepperkok R. (2005)
Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources
Meth. Enzymol. 404:19-26. doi:10.1016/s0076-6879(05)04003-6
Europe PMC | doi

Watson P, Forster R, Palmer KJ, Pepperkok R, Stephens DJ. (2005)
Coupling of ER exit to microtubules through direct interaction of COPII with dynactin
Nat. Cell Biol. 7(1):48-55. doi:10.1038/ncb1206
Europe PMC | doi

Terjung S, Pepperkok R, Ankerhold R, Caiolfa VR, Castel S, Majer P, Geiger B, Luini A, Salamero J, Shorte S, Small JV. (2005)
EAMNET - a resource and teaching framework for advanced light microscopy
Eur. J. Cell Biol. 84:108.
Europe PMC

Reynaud EG, Andrade MA, Bonneau F, Ly TB, Knop M, Scheffzek K, Pepperkok R. (2005)
Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization
BMC Biol. 3:21. doi:10.1186/1741-7007-3-21
Europe PMC | doi

Pepperkok R, Simpson JC, Rietdorf J, Cetin C, Liebel U, Terjung S, Zimmermann T. (2005)
Imaging platforms for measurement of membrane trafficking
Meth. Enzymol. 404:8-18. doi:10.1016/s0076-6879(05)04002-4
Europe PMC | doi

Moss DA, Keese M, Pepperkok R. (2005)
IR micro spectroscopy of live cells
Vib Spectrosc 38(1-2):185-191.
Europe PMC

Ben-Tekaya H, Miura K, Pepperkok R, Hauri HP. (2005)
Live imaging of bidirectional traffic from the ERGIC
J. Cell. Sci. 118(Pt 2):357-367. doi:10.1242/jcs.01615
Europe PMC | doi

Arlt D, Huber W, Sauermann M, Majety M, Hahne F, Pepperkok R, Poustka A, Wiemann S. (2005)
Novel cancer relevant cell cycle modulators identified in automated cell-based assays
Paper presented at the 30 Jahre Deutsche Gesellschaft für Zellbiologie - Jahrestagung, Heidelberg, 16 - 19 March 2005.Eur. J. Cell Biol. 84:30.
Europe PMC

Young J, Stauber T, del Nery E, Vernos I, Pepperkok R, Nilsson T. (2005)
Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'
Mol. Biol. Cell 16(1):162-177. doi:10.1091/mbc.e04-03-0260
Europe PMC | doi


Brunet S, Sardon T, Zimmerman T, Wittmann T, Pepperkok R, Karsenti E, Vernos I. (2004)
Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts
Mol. Biol. Cell 15(12):5318-5328. doi:10.1091/mbc.e04-05-0385
Europe PMC | doi

Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A. (2004)
From ORFeome to biology: a functional genomics pipeline
Genome Res. 14(10B):2136-2144. doi:10.1101/gr.2576704
Europe PMC | doi

Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok R. (2004)
High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
Genome Res. 14(10A):1948-1956. doi:10.1101/gr.2658304
Europe PMC | doi

Erfle H, Simpson JC, Bastiaens PI, Pepperkok R. (2004)
siRNA cell arrays for high-content screening microscopy
BioTechniques 37(3):454-8, 460, 462.
Europe PMC

Liebel U, Kindler B, Pepperkok R. (2004)
'Harvester': a fast meta search engine of human protein resources
Bioinformatics 20(12):1962-1963. doi:10.1093/bioinformatics/bth146
Europe PMC | doi

Becker S, Simpson JC, Pepperkok R, Heinz S, Herder C, Grez M, Seifried E, Tonn T. (2004)
Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells
Thromb. Haemost. 92(1):23-35. doi:10.1267/thro04070023
Europe PMC | doi

Stephens DJ, Pepperkok R. (2004)
Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum
J. Cell. Sci. 117(Pt 16):3635-3644. doi:10.1242/jcs.01269
Europe PMC | doi