We have developed a number of RNAi transfection protocols (Erfle et al.), which can be also used for plasmids.
These protocols are based on solid phase reverse transfection and can be used on multi-well plates or LabTek slides. The ALMF provides equipment for coating such plates or LabTeks.
We can offer support with:
- siRNA design
- Selection of available or customized siRNA libraries
- siRNA plate layout
- RNAi transfection
- Bioinformatic analysis of siRNA sequences
In addition, we run siRNA design courses (internal only) on a regular basis.