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Advanced Light Microscopy FacilityServices

  • Assisting all EMBL groups and visitors throughout the process of microscopic imaging:
    planning of a project, sample preparation, selection and usage of the microscope, image processing and visualisation, data transfer and storage.
  • In collaboration with our technology partners we offer and support advanced microscopy techniques
    • Optical sectioning: confocal and  two-photon laser scanning microscopes, spinning disk microscopy, total internal reflection (TIRF)
    • Molecular dynamics and interactions: FRAP, FRET, FLIM, FCS
    • Super-resolution: 3D single-molecule localisation (GSDIM / dSTORM) and 3D gated stimulated emission depletion (gSTED)
    • Laser nanosurgery: Pulsed lasers, which are focused diffraction limited on the sample can be used to cut fine intracellular structures (e.g. actin or microtubules). The laser is positioned on the sample by a scanner. It can be also used on larger structures like to ablate groups of cells.
    • Photo-activation, photo-uncaging
  • We assist our users in all aspects of high-throughput microscopy
  • We offer image and data analysis support as well as commercial and customised open source image and data analysis software.
  • Facilitating hands on evaluation of equipment in collaboration with technology partners.
  • Developing accessory software and microscopy equipment (co-developments with industrial partners, pre-evaluation of commercial equipment).
  • Organising courses (different EMBO courses per year).
  • Useful links and downloads concerning microscopy and image analysis.