Top image

Electron Microscopy Core FacilityEquipment

Electron Microscopes

Morgagni FEI - equipped with a side-mounted 1K CCD Camera (SIS)
Location: Room 318
(Morgagni)

Zeiss EM10 - equipped with a side-mounted 1K CCD Camera (Gatan)
Location: Room 317
(Zeiss EM10)

Biotwin CM120 Philips - equipped with a bottom-mounted 1K CCD Camera (Keen View, SIS)
Location: Room 305C
(Biotwin)

Biotwin Ice CM120 Philips (cryomicroscope) - equipped with a bottom-mounted 1K CCD camera (GATAN)
Location: Room 316
(Biotwin ice)

Philips CM200 (cryomicroscope and plastic microscope) - FEG microscope equipped with a bottom-mounted 2K CCD Camera (Tietz) and a with a tomography software (Tietz).
Location: Room 319
(CM200)

POLARA, 300kV FEG, FEI, tomography-cryomicroscope (exclusively) for high resolution molecular structure analysis. Equipped with Energy Filter and 2K CCD camera.
Location: Room 320
(Polara)

TECNAI F30, 300kV FEG, FEI, tomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography applications (plastic samples)
Location: Room 322
(F30)

Titan Krios, 300kV FEG, FEI. Cryo-microscope for high-resolution structural analysis and tomography. Equipped with 4K CCD camera, Energy Filter and 2K CCD camera, automatic loading and refilling systems.
Location: Room 312

Ultramicrotomes (R 325)

For plastic sections

Conventional Ultramicrotomes (LEICA)

  • Ultracut UCT
    (plastic-1)

  • Ultracut S
    (plastic-2)

For cryosections

Cryo-ultra-microtomes for Tokuyasu cryosections (Leica)

  • Ultracut UCT
    (cryo-2)

  • UC6
    (cryo-3)

Cryo-ultra-microtome for frozen hydrated sections (Leica)

  • UC6
    (cryo-1)
    located in a box with temperature and humidity control

Accessory equipment

1 Knife-maker, used to prepare glass knives for ultramicrotomy (Leica)
Location: Room 325

Carbon coaters
BALTEC BAE250
BALTEC MED 020
Location: Room 324

Glow discharge device
Location: Room 324

Cryogenic equipment

High Pressure Freezer

Freeze Substitution Devices (FS), 4 items:

  • AFS-1, process samples for manual freeze-substitution and plastic-embedding
    (AFS-1)
  • AFS-2a, process samples for automated freeze-substitution and plastic embedding
    (AFS-2a)
  • AFS-2b, process samples for automated freeze-substitution and plastic embedding
    (AFS-2b)
  • AFS-2c, process samples for automated freeze-substitution and plastic embedding
    (AFS-2c): acquired with the support of the HMLS