Staff at EMBL

Alexis Perez Gonzalez

Manager Flow Cytometry Core Facility
Flow Cytometry

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Biography

PhD 2003, Center of Molecular Immunology & University of Havana, Cuba.
Postdoctoral research and cytometry lab manager at Gulbenkian Institute of Science, Oeiras, Portugal.
At EMBL since 2006. Facility Manager since 2012.

Publications

Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP. Bonn, S., Zinzen, R.P., Perez-Gonzalez, A., Riddell, A., Gavin, A.C. & Furlong, E.E. Nat Protoc. 2012 Apr 26;7(5):978-94. doi: 10.1038/nprot.2012.049. PubMed

Tissue-specific analysis of chromatin state identifies temporal signatures of enhancer activity during embryonic development. Bonn, S., Zinzen, R.P., Girardot, C., Gustafson, E.H., Perez-Gonzalez, A., Delhomme, N., Ghavi-Helm, Y., Wilczynski, B., Riddell, A. & Furlong, E.E. Nat Genet. 2012 Jan 8;44(2):148-56. doi: 10.1038/ng.1064. PubMed

Regulatory T cells contribute to diabetes protection in lipopolysaccharide-treated non-obese diabetic mice. Caramalho, I., Rodrigues-Duarte, L., Perez, A., Zelenay, S., Penha-Goncalves, C. & Demengeot, J. Scand J Immunol. 2011 Dec;74(6):585-95. doi: 10.1111/j.1365-3083.2011.02627.x. PubMed

Sphingosine kinase-1 (SphK-1) regulates Mycobacterium smegmatis infection in macrophages. Prakash, H., Luth, A., Grinkina, N., Holzer, D., Wadgaonkar, R., Gonzalez, A.P., Anes, E. & Kleuser, B. PLoS One. 2010 May 17;5(5):e10657. doi: 10.1371/journal.pone.0010657. PubMed

Role of lipids in killing mycobacteria by macrophages: evidence for NF-kappaB-dependent and -independent killing induced by different lipids. Gutierrez, M.G., Gonzalez, A.P., Anes, E. & Griffiths, G. Cell Microbiol. 2009 Mar;11(3):406-20. Epub 2008 Nov 7. PubMed

Generation and characterization of an anti-idiotype monoclonal antibody related to GM3(NeuGc) ganglioside. Rodriguez, M., Llanes, L., Perez, A., Perez, R. & Vazquez, A.M. Hybrid Hybridomics. 2003 Oct;22(5):307-14. PubMed

Chimeric anti-N-glycolyl-ganglioside and its anti-idiotypic MAbs: immunodominance of their variable regions. Lopez-Requena, A., Mateo de Acosta, C., Perez, A., Valle, A., Lombardero, J., Sosa, K., Perez, R. & Vazquez, A.M. Hybrid Hybridomics. 2003 Aug;22(4):235-43. PubMed

A monoclonal antibody against NeuGc-containing gangliosides contains a regulatory idiotope involved in the interaction with B and T cells. Perez, A., Mier, E.S., Vispo, N.S., Vazquez, A.M. & Perez Rodriguez, R. Mol Immunol. 2002 Sep;39(1-2):103-12. PubMed

An anti-idiotype vaccine elicits a specific response to N-glycolyl sialic acid residues of glycoconjugates in melanoma patients. Alfonso, M., Diaz, A., Hernandez, A.M., Perez, A., Rodriguez, E., Bitton, R., Perez, R. & Vazquez, A.M. J Immunol. 2002 Mar 1;168(5):2523-9. PubMed

Immunogenetic analysis of variable regions encoding AB1 and gamma-type AB2 antibodies from the NeuGc-containing ganglioside family. Perez, A., Lombardero, J., Mateo, C., Mustelier, G., Alfonso, M., Vazquez, A.M. & Perez, R. Hybridoma. 2001 Aug;20(4):211-21. PubMed

Syngeneic anti-idiotypic monoclonal antibodies to an anti-NeuGc-containing ganglioside monoclonal antibody. Vazquez, A.M., Perez, A., Hernandez, A.M., Macias, A., Alfonso, M., Bombino, G. & Perez, R. Hybridoma. 1998 Dec;17(6):527-34. PubMed