Top image

Staff at EMBL

Alexis Perez Gonzalez

Manager Flow Cytometry Core Facility
Flow Cytometry

EMBL Heidelberg, Meyerhofstra├če 1, 69117 Heidelberg, Germany

E-mail: send a mail

Biography

PhD 2003, Center of Molecular Immunology & University of Havana, Cuba.
Postdoctoral research and cytometry lab manager at Gulbenkian Institute of Science, Oeiras, Portugal.
At EMBL since 2006. Facility Manager since 2012.

Publications

Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. Mahen, R., Koch, B., Wachsmuth, M., Politi, A.Z., Perez-Gonzalez, A., Mergenthaler, J., Cai, Y. & Ellenberg, J. Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-06-1091. Europe PMC

Sequencing of a patient with balanced chromosome abnormalities and neurodevelopmental disease identifies disruption of multiple high risk Loci by structural variation. Blake, J., Riddell, A., Theiss, S., Gonzalez, A.P., Haase, B., Jauch, A., Janssen, J.W., Ibberson, D., Pavlinic, D., Moog, U., Benes, V. & Runz, H. PLoS One. 2014 Mar 13;9(3):e90894. doi: 10.1371/journal.pone.0090894. eCollection2014. Europe PMC

Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP. Bonn, S., Zinzen, R.P., Perez-Gonzalez, A., Riddell, A., Gavin, A.C. & Furlong, E.E. Nat Protoc. 2012 Apr 26;7(5):978-94. doi: 10.1038/nprot.2012.049. Europe PMC

Tissue-specific analysis of chromatin state identifies temporal signatures of enhancer activity during embryonic development. Bonn, S., Zinzen, R.P., Girardot, C., Gustafson, E.H., Perez-Gonzalez, A., Delhomme, N., Ghavi-Helm, Y., Wilczynski, B., Riddell, A. & Furlong, E.E. Nat Genet. 2012 Jan 8;44(2):148-56. doi: 10.1038/ng.1064. Europe PMC

Regulatory T cells contribute to diabetes protection in lipopolysaccharide-treated non-obese diabetic mice. Caramalho, I., Rodrigues-Duarte, L., Perez, A., Zelenay, S., Penha-Goncalves, C. & Demengeot, J. Scand J Immunol. 2011 Dec;74(6):585-95. doi: 10.1111/j.1365-3083.2011.02627.x. Europe PMC

Sphingosine kinase-1 (SphK-1) regulates Mycobacterium smegmatis infection in macrophages. Prakash, H., Luth, A., Grinkina, N., Holzer, D., Wadgaonkar, R., Gonzalez, A.P., Anes, E. & Kleuser, B. PLoS One. 2010 May 17;5(5):e10657. doi: 10.1371/journal.pone.0010657. Europe PMC

Role of lipids in killing mycobacteria by macrophages: evidence for NF-kappaB-dependent and -independent killing induced by different lipids. Gutierrez, M.G., Gonzalez, A.P., Anes, E. & Griffiths, G. Cell Microbiol. 2009 Mar;11(3):406-20. Epub 2008 Nov 7. Europe PMC

Generation and characterization of an anti-idiotype monoclonal antibody related to GM3(NeuGc) ganglioside. Rodriguez, M., Llanes, L., Perez, A., Perez, R. & Vazquez, A.M. Hybrid Hybridomics. 2003 Oct;22(5):307-14. Europe PMC

Chimeric anti-N-glycolyl-ganglioside and its anti-idiotypic MAbs: immunodominance of their variable regions. Lopez-Requena, A., Mateo de Acosta, C., Perez, A., Valle, A., Lombardero, J., Sosa, K., Perez, R. & Vazquez, A.M. Hybrid Hybridomics. 2003 Aug;22(4):235-43. Europe PMC

A monoclonal antibody against NeuGc-containing gangliosides contains a regulatory idiotope involved in the interaction with B and T cells. Perez, A., Mier, E.S., Vispo, N.S., Vazquez, A.M. & Perez Rodriguez, R. Mol Immunol. 2002 Sep;39(1-2):103-12. Europe PMC

An anti-idiotype vaccine elicits a specific response to N-glycolyl sialic acid residues of glycoconjugates in melanoma patients. Alfonso, M., Diaz, A., Hernandez, A.M., Perez, A., Rodriguez, E., Bitton, R., Perez, R. & Vazquez, A.M. J Immunol. 2002 Mar 1;168(5):2523-9. Europe PMC

Immunogenetic analysis of variable regions encoding AB1 and gamma-type AB2 antibodies from the NeuGc-containing ganglioside family. Perez, A., Lombardero, J., Mateo, C., Mustelier, G., Alfonso, M., Vazquez, A.M. & Perez, R. Hybridoma. 2001 Aug;20(4):211-21. Europe PMC

Syngeneic anti-idiotypic monoclonal antibodies to an anti-NeuGc-containing ganglioside monoclonal antibody. Vazquez, A.M., Perez, A., Hernandez, A.M., Macias, A., Alfonso, M., Bombino, G. & Perez, R. Hybridoma. 1998 Dec;17(6):527-34. Europe PMC