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Staff at EMBL

CP-60003254

Dr. Hüseyin Besir

Head of Protein Expression and Purification Core Facility
Protein Expression and Purification Core Facility

EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

E-mail: send a mail

Biography

PhD 2001, Max Planck Institute of Biochemistry, Munich.
Postdoctoral research at Roche Diagnostics, Penzberg, and the Max Planck Institute of Biochemistry, Munich.
Facility head at EMBL since 2006.

Publications

Total: 15 publication(s)

2013

Costa SJ, Almeida A, Castro A, Domingues L, Besir H. (2013)
The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology
Appl. Microbiol. Biotechnol. 97(15):6779-6791. doi:10.1007/s00253-012-4559-1
Europe PMC | doi

Scholz J, Besir H, Strasser C, Suppmann S. (2013)
A new method to customize protein expression vectors for fast, efficient and background free parallel cloning
BMC Biotechnol. 13:12. doi:10.1186/1472-6750-13-12
Europe PMC | doi

Ventura E, Riondato M, Sambuceti G, Salis A, Damonte G, Cordazzo C, Besir H, Pistoia V, Zardi L. (2013)
Use of the uteroglobin platform for the expression of a bivalent antibody against oncofetal fibronectin in Escherichia coli
PLoS ONE 8(12):e82878. doi:10.1371/journal.pone.0082878
Europe PMC | doi


2012

Gjerstorff MF, Rösner HI, Pedersen CB, Greve KB, Schmidt S, Wilson KL, Mollenhauer J, Besir H, Poulsen FM, Møllegaard NE, Ditzel HJ. (2012)
GAGE cancer-germline antigens are recruited to the nuclear envelope by germ cell-less (GCL)
PLoS ONE 7(9):e45819. doi:10.1371/journal.pone.0045819
Europe PMC | doi

Schlesner M, Miller A, Besir H, Aivaliotis M, Streif J, Scheffer B, Siedler F, Oesterhelt D. (2012)
The protein interaction network of a taxis signal transduction system in a halophilic archaeon
BMC Microbiol. 12:272. doi:10.1186/1471-2180-12-272
Europe PMC | doi


2011

Bayer-Giraldi M, Weikusat I, Besir H, Dieckmann G. (2011)
Characterization of an antifreeze protein from the polar diatom Fragilariopsis cylindrus and its relevance in sea ice
Cryobiology 63(3):210-219. doi:10.1016/j.cryobiol.2011.08.006
Europe PMC | doi


2010

Gjerstorff MF, Besir H, Larsen MR, Ditzel HJ. (2010)
Expression, purification and characterization of the cancer-germline antigen GAGE12I: a candidate for cancer immunotherapy
Protein Expr. Purif. 73(2):217-222. doi:10.1016/j.pep.2010.05.010
Europe PMC | doi


2009

Lawrence AM, Besir HU. (2009)
Staining of proteins in gels with Coomassie G-250 without organic solvent and acetic acid
J Vis Exp (30):351-356. doi:10.3791/1350
Europe PMC | doi


2008

Hanke S, Besir H, Oesterhelt D, Mann M. (2008)
Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level
J. Proteome Res. 7(3):1118-1130. doi:10.1021/pr7007175
Europe PMC | doi

Tarasov VY, Besir H, Schwaiger R, Klee K, Furtwängler K, Pfeiffer F, Oesterhelt D. (2008)
A small protein from the bop-brp intergenic region of Halobacterium salinarum contains a zinc finger motif and regulates bop and crtB1 transcription
Mol. Microbiol. 67(4):772-780. doi:10.1111/j.1365-2958.2007.06081.x
Europe PMC | doi


2007

Klein C, Aivaliotis M, Olsen JV, Falb M, Besir H, Scheffer B, Bisle B, Tebbe A, Konstantinidis K, Siedler F, Pfeiffer F, Mann M, Oesterhelt D. (2007)
The low molecular weight proteome of Halobacterium salinarum
J. Proteome Res. 6(4):1510-1518. doi:10.1021/pr060634q
Europe PMC | doi


2006

Sapra KT, Besir H, Oesterhelt D, Muller DJ. (2006)
Characterizing molecular interactions in different bacteriorhodopsin assemblies by single-molecule force spectroscopy
J. Mol. Biol. 355(4):640-650. doi:10.1016/j.jmb.2005.10.080
Europe PMC | doi


2005

Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D. (2005)
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum
FEBS Lett. 579(29):6595-6600. doi:10.1016/j.febslet.2005.10.052
Europe PMC | doi

Deverall MA, Gindl E, Sinner EK, Besir H, Ruehe J, Saxton MJ, Naumann CA. (2005)
Membrane lateral mobility obstructed by polymer-tethered lipids studied at the single molecule level
Biophys. J. 88(3):1875-1886. doi:10.1529/biophysj.104.050559
Europe PMC | doi


2000

Kolbe M, Besir H, Essen LO, Oesterhelt D. (2000)
Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution
Science 288(5470):1390-1396. doi:10.1126/science.288.5470.1390
Europe PMC | doi