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Protein Expression and Purification Core FacilityPublications

Use of the Uteroglobin Platform for the Expression of a Bivalent Antibody against Oncofetal Fibronectin in Escherichia coli.
Ventura, E., Riondato, M., Sambuceti, G., Salis, A., Damonte, G., Cordazzo, C., Besir, H., Pistoia, V. & Zardi, L.
PLoS One. 2013 Dec 19;8(12):e82878. doi: 10.1371/journal.pone.0082878. Europe PMC

The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology.
Costa, S.J., Almeida, A., Castro, A., Domingues, L. & Besir, H.
Appl Microbiol Biotechnol. 2013 Aug;97(15):6779-91. doi:10.1007/s00253-012-4559-1. Epub 2012 Nov 20. Europe PMC

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.
Scholz, J., Besir, H., Strasser, C. & Suppmann, S.
BMC Biotechnol. 2013 Feb 14;13:12. doi: 10.1186/1472-6750-13-12. Europe PMC

The protein interaction network of a taxis signal transduction system in a Halophilic Archaeon.
Schlesner, M., Miller, A., Besir, H., Aivaliotis, M., Streif, J., Scheffer, B., Siedler, F. & Oesterhelt, D.
BMC Microbiol. 2012 Nov 21;12:272. doi: 10.1186/1471-2180-12-272. Europe PMC

GAGE Cancer-Germline Antigens Are Recruited to the Nuclear Envelope by Germ Cell-Less (GCL).
Gjerstorff, M.F., Rosner, H.I., Pedersen, C.B., Greve, K.B., Schmidt, S., Wilson, K.L., Mollenhauer, J., Besir, H., Poulsen, F.M., Mollegaard, N.E. & Ditzel, H.J.
PLoS One. 2012;7(9):e45819. doi: 10.1371/journal.pone.0045819. Epub 2012 Sep 20. Europe PMC

Characterization of an antifreeze protein from the polar diatom Fragilariopsis cylindrus and its relevance in sea ice.
Bayer-Giraldi, M., Weikusat, I., Besir, H. & Dieckmann, G.
Cryobiology. 2011 Dec;63(3):210-9. Epub 2011 Aug 26. Europe PMC

Expression, purification and characterization of the cancer-germline antigen GAGE12I: a candidate for cancer immunotherapy.
Gjerstorff, M.F., Besir, H., Larsen, M.R. & Ditzel, H.J.
Protein Expr Purif. 2010 Oct;73(2):217-22. Epub 2010 May 27. Europe PMC

Staining of proteins in gels with Coomassie G-250 without organic solvent and acetic acid.
Lawrence, A.M. & Besir, H.U.
J Vis Exp. 2009 Aug 14;(30). pii: 1350. doi: 10.3791/1350. Europe PMC

Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level.
Hanke, S., Besir, H., Oesterhelt, D. & Mann, M.
J Proteome Res. 2008 Mar;7(3):1118-30. Epub 2008 Feb 14. Europe PMC

A small protein from the bop-brp intergenic region of Halobacterium salinarum contains a zinc finger motif and regulates bop and crtB1 transcription.
Tarasov, V.Y., Besir, H., Schwaiger, R., Klee, K., Furtwangler, K., Pfeiffer, F. & Oesterhelt, D.
Mol Microbiol. 2008 Feb;67(4):772-80. Epub 2008 Jan 2. Europe PMC

The low molecular weight proteome of Halobacterium salinarum.
Klein, C., Aivaliotis, M., Olsen, J.V., Falb, M., Besir, H., Scheffer, B., Bisle, B., Tebbe, A., Konstantinidis, K., Siedler, F., Pfeiffer, F., Mann, M. & Oesterhelt, D.
J Proteome Res. 2007 Apr;6(4):1510-8. Epub 2007 Feb 28. Europe PMC

Characterizing molecular interactions in different bacteriorhodopsin assemblies by single-molecule force spectroscopy.
Sapra, K.T., Besir, H., Oesterhelt, D. & Muller, D.J.
J Mol Biol. 2006 Jan 27;355(4):640-50. Epub 2005 Nov 17. Europe PMC

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum.
Besir, H., Zeth, K., Bracher, A., Heider, U., Ishibashi, M., Tokunaga, M. & Oesterhelt, D.
FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. Europe PMC

Membrane lateral mobility obstructed by polymer-tethered lipids studied at the single molecule level.
Deverall, M.A., Gindl, E., Sinner, E.K., Besir, H., Ruehe, J., Saxton, M.J. & Naumann, C.A.
Biophys J. 2005 Mar;88(3):1875-86. Epub 2004 Dec 21. Europe PMC

Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution.
Kolbe, M., Besir, H., Essen, L.O. & Oesterhelt, D.
Science. 2000 May 26;288(5470):1390-6. Europe PMC