Top image

Proteomics Core Facility

The Proteomics Core Facility provides a full proteomics infrastructure for the identification and characterisation of proteins.

Infrastructure in the Proteomics Core Facility is centered around state-of-the-art mass spectrometry for MS and LC-MSMS experiments.  This is complemented by chromatographic and electrophoretic systems for protein and peptide separation.

Major projects and developments

  • Molecular weight determination of intact proteins.
  • Routine identification of proteins from coomassie and silver-stained gels.
  • Identification of post-translational modifications.
  • Nano flow liquid chromatography coupled to high resolution mass spectrometry: (LC-MSMS) for the identification of proteins in complex mixtures.
  • Protein quantification by stable-isotope labelling (e.g. SILAC).

Services provided

Proteomics:

  • Protein identification from gel or in solution.
  • High resolution and high mass-accuracy MS, MSMS, and LC-MSMS (Thermo Orbitrap Velos Pro and Q-Exactive) for identification and quantification of proteins in complex mixtures.
  • Ion trap (Bruker HCT) LC-MSMS for routine identification of proteins from coomassie and silver-stained gels.
  • Triple-quad mass spectrometry (Thermo Vantage) for targeted protein analysis.
  • Protein quantification by stable-isotope labeling (SILAC, TMT and dimethyl labelling).
  • Identification of post-translational modifications.
  • Enrichment of phosphopeptides (TiO2 and IMAC).
  • Multi-dimensional peptide separation (isoelectric focusing and liquid chromatography).

Analysis of intact proteins:

  • Molecular weight determination of intact proteins by ESI mass spectrometry.
  • Determination of N- and C-termini of proteins and products of limited proteolysis.
  • Verification of incorporation of non-natural amino acids.

Technology partner

  • BIO-RAD