Proteomics Core Facility
To address the needs of the post genomic era, the Proteomic Core Facility was set up as a cooperation between EMBL and industry. We provide a complete functional proteomic line, including medium-throughput protein and peptide identification, for both in-house service and external visitors.
Major projects and developments
- Completed differential proteomics of wild type and mutant E. coli (in cooperation with EMBL Hamburg).
- Identified marker proteins for the rare cerebrovascular disorder Moya Moya, which affects
the carotid circulation of the brain (in cooperation with the University Hospital
Mannheim). - Developed new MALDI target coating for direct on-target sample preparation.
Technology partners
BIO-RAD – places advanced equipment at the facility's disposal, including the complete Proteome Works System, 2D-gel equipment, spot cutting robot, HPLC, Photometer, Fluorescence Imager and Densitometer.
Services provided
- Preparative HPLC protein purification.
- IEF with different gradient ranges.
- 2D-gel electrophoresis including colloidal Coomassie, silver or fluorescence staining.
- Laser fluorescence imaging and quantification.
- High-resolution and high-sensitivity imaging densitometer for silver and Coomassie staining.
- PDQuest analysis and evaluation.
- Automatic gel spot excision (fluorescence, silver and Coomassie).
- Full automatic in gel digestion and MALDI target spotting.
- MALDI peptide mass fingerprinting and online database protein identification.
- MS and MS/MS nano electrospray of proteins and peptides.
- Online nano-LC-MS/MS protein identification and CID/ETD analysis of post-translational modifications.