Proteomics Core Facility
The Proteomics Core Facility provides a full proteomics infrastructure for the identification and characterisation of proteins.
Infrastructure in the facility is centered on state-of-the-art mass spectrometry for MS and LC-MSMS experiments. It includes chromatographic and electrophoretic systems for protein and peptide separation.
Major projects and developments
- Molecular weight determination of intact proteins.
- Routine identification of proteins from coomassie and silver-stained gels.
- Identification of post-translational modifications.
- Nano-flow liquid chromatography coupled to high-resolution mass spectrometry: (LC-MSMS) for identification of proteins in complex mixtures.
- Protein quantification by stable-isotope labelling (e.g. SILAC).
Services provided
Analysis of intact proteins:
- Molecular weight determination of intact proteins by ESI mass spectrometry (Waters Q-tof).
- Determination of N- and C-termini of proteins and products of limited proteolysis.
- Verify incorporation of non-natural amino acids.
Proteomics:
- Protein identification from gel or in solution.
- High-resolution and high mass accuracy MS, MSMS, and LC-MSMS (Thermo Orbitrap Velos Pro) for identification and quantification of proteins in complex mixtures.
- Ion trap (Bruker HCT) LC-MSMS for routine identification of proteins from coomassie and silver-stained gels.
- Protein quantification by stable-isotope labelling (SILAC and dimethyl labelling).
- Identification of post-translational modifications.
- Enrichment of phosphopeptides (TiO2 and IMAC).
- Multi-dimensional peptide separation (isoelectric focusing and liquid chromatography)
Technology partner
- BIO-RAD