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EMBO Practical Course

Genomic Approaches to Evolution and Development

EMBL Heidelberg, Germany

Sunday 7 February - Thursday 11 February 2010

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Programme

Day one: Chromatin preparation, initiate a chromatin immunoprecipitation (ChIP)

Day two: Second day of the ChIP protocol, initiate a Seq-Solexa library preparation

Day three: Third day of ChIP, size select the Solexa library and PCR enrich adapter modified DNA

Day four: Complete the ChIP, quality control using real-time PCR amplify quality control of Solexa library preparation (bioanalyzer), analyze qPCR results

Day five: Introduction to computational analysis of ChIP-seq results