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Microinjection into adherent cells- theory and practical exercises

EMBL Heidelberg Wednesday 14 April - Thursday 15 April 2010
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Programme

Preliminary Programme

Wednesday, 14 April 2010
Introduction to microinjection and fundamentals of light- and fluorescence microscopy.

Practical 1: Correct usage of microscope and contrast techniques

Practical 2: Sample preparation and injection of fluorescent dyes

Thursday, 15 April 2010
Microinjection and accurate Microscopy analysis.

Practical 3: Injection of different GFP vectors

Practical 4: Results analysis

Troubleshooting